+Open data
-Basic information
Entry | Database: PDB / ID: 6i19 | ||||||
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Title | Crystal structure of Chlamydomonas reinhardtii thioredoxin h1 | ||||||
Components | Thioredoxin H-type | ||||||
Keywords | ELECTRON TRANSPORT / h-type cytosolic thioredoxin oxidized | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Chlamydomonas reinhardtii (plant) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.378 Å | ||||||
Authors | Lemaire, S.D. / Tedesco, D. / Crozet, P. / Michelet, L. / Fermani, S. / Zaffagnini, M. / Henri, J. | ||||||
Citation | Journal: Antioxidants (Basel) / Year: 2018 Title: Crystal Structure of Chloroplastic Thioredoxin f2 fromChlamydomonas reinhardtiiReveals Distinct Surface Properties. Authors: Lemaire, S.D. / Tedesco, D. / Crozet, P. / Michelet, L. / Fermani, S. / Zaffagnini, M. / Henri, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6i19.cif.gz | 62.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6i19.ent.gz | 45.2 KB | Display | PDB format |
PDBx/mmJSON format | 6i19.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/i1/6i19 ftp://data.pdbj.org/pub/pdb/validation_reports/i1/6i19 | HTTPS FTP |
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-Related structure data
Related structure data | 6i1cC 1ep7S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 11858.730 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chlamydomonas reinhardtii (plant) / Gene: TRXH / Production host: Escherichia coli (E. coli) / References: UniProt: P80028 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.08 Å3/Da / Density % sol: 40.95 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 200 mM ammonium sulfate, 100 mM sodium cacodylate, pH 6.5, 30% PEG 8000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 1 / Wavelength: 0.9677 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Sep 7, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9677 Å / Relative weight: 1 |
Reflection | Resolution: 1.378→36.42 Å / Num. obs: 41955 / % possible obs: 99.8 % / Redundancy: 2 % / Net I/σ(I): 25.45 |
Reflection shell | Resolution: 1.378→1.427 Å |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1ep7 Resolution: 1.378→36.42 Å / SU ML: 0.13 / Cross valid method: FREE R-VALUE / σ(F): 1.39 / Phase error: 21.83
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.378→36.42 Å
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Refine LS restraints |
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LS refinement shell |
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