+Open data
-Basic information
Entry | Database: PDB / ID: 6i0q | ||||||
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Title | Crystal structure of BTB domain of KCTD16 hexamer | ||||||
Components | BTB/POZ domain-containing protein KCTD16 | ||||||
Keywords | STRUCTURAL PROTEIN / KCTD / GABA(B) receptor / Receptor associated protein | ||||||
Function / homology | Function and homology information regulation of G protein-coupled receptor signaling pathway / cell projection / protein homooligomerization / presynaptic membrane / postsynaptic membrane / receptor complex Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å | ||||||
Authors | Kasaragod, V.B. / Sereikaite, V. / Stromgaard, K. / Schindelin, H. | ||||||
Funding support | Germany, 1items
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Citation | Journal: J.Med.Chem. / Year: 2019 Title: Targeting the gamma-Aminobutyric Acid Type B (GABAB) Receptor Complex: Development of Inhibitors Targeting the K+Channel Tetramerization Domain (KCTD) Containing Proteins/GABABReceptor Protein-Protein Interaction. Authors: Sereikaite, V. / Fritzius, T. / Kasaragod, V.B. / Bader, N. / Maric, H.M. / Schindelin, H. / Bettler, B. / Stromgaard, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6i0q.cif.gz | 52.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6i0q.ent.gz | 37.7 KB | Display | PDB format |
PDBx/mmJSON format | 6i0q.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/i0/6i0q ftp://data.pdbj.org/pub/pdb/validation_reports/i0/6i0q | HTTPS FTP |
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-Related structure data
Related structure data | 5a15S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 12176.007 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: KCTD16, KIAA1317 / Production host: Escherichia coli (E. coli) / References: UniProt: Q68DU8 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.93 Å3/Da / Density % sol: 36.36 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.2 M ammonium sulfate and 20% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-2 / Wavelength: 0.8 Å |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Jun 3, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→41.86 Å / Num. obs: 4228 / % possible obs: 100 % / Redundancy: 9.1 % / CC1/2: 0.994 / Rmerge(I) obs: 0.2 / Rpim(I) all: 0.104 / Rrim(I) all: 0.229 / Net I/σ(I): 8.1 |
Reflection shell | Resolution: 2.3→2.38 Å / Redundancy: 9.4 % / Rmerge(I) obs: 1.372 / Mean I/σ(I) obs: 1.8 / Num. unique obs: 403 / CC1/2: 0.54 / Rpim(I) all: 0.69 / Rrim(I) all: 1.54 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5A15 Resolution: 2.3→33.095 Å / SU ML: 0.32 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 15.71
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→33.095 Å
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Refine LS restraints |
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LS refinement shell |
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