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Open data
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Basic information
Entry | Database: PDB / ID: 6hfg | ||||||
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Title | Structure of the REC114 PH domain | ||||||
![]() | Meiotic recombination protein REC114 | ||||||
![]() | RECOMBINATION / Pleckstrin Homology domain Meiotic recombination | ||||||
Function / homology | Meiotic recombination protein REC114-like / Meiotic recombination protein REC114-like / meiotic DNA double-strand break formation / oogenesis / chromosome / spermatogenesis / DNA recombination / Meiotic recombination protein REC114![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Juarez-Martinez, A.B. / de Massy, B. / Kadlec, J. | ||||||
![]() | ![]() Title: Mouse REC114 is essential for meiotic DNA double-strand break formation and forms a complex with MEI4. Authors: Kumar, R. / Oliver, C. / Brun, C. / Juarez-Martinez, A.B. / Tarabay, Y. / Kadlec, J. / de Massy, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 36.7 KB | Display | ![]() |
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PDB format | ![]() | 24 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 428.3 KB | Display | ![]() |
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Full document | ![]() | 428.6 KB | Display | |
Data in XML | ![]() | 6.3 KB | Display | |
Data in CIF | ![]() | 7.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16239.387 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.25 Å3/Da / Density % sol: 71.07 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 0.25 M ammonium sulphate, 0.1 M MES (pH 6.5), 28% PEG 5000 MME |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 2M / Detector: PIXEL / Date: Oct 29, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.966 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→93 Å / Num. obs: 10262 / % possible obs: 99.6 % / Observed criterion σ(I): 1.1 / Redundancy: 11.2 % / Rmerge(I) obs: 0.057 / Net I/σ(I): 24.2 |
Reflection shell | Resolution: 2.5→2.57 Å / Redundancy: 11.1 % / Mean I/σ(I) obs: 1.1 / Num. unique obs: 749 / CC1/2: 0.548 / % possible all: 97.5 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 77.071 Å2
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Refinement step | Cycle: 1 / Resolution: 2.5→46.59 Å
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Refine LS restraints |
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