Mass: 11784.570 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli), (gene. exp.) Sulfolobus solfataricus (archaea) Strain: K12, ATCC 35092 / DSM 1617 / JCM 11322 / P2 / Gene: trxA, fipA, tsnC, b3781, JW5856, trxA-1, SSO0368 / Production host: Escherichia coli (E. coli) / References: UniProt: P0AA25, UniProt: Q980E5
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 4.41 Å3/Da / Density % sol: 72.13 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop Details: Crystals were obtained with a protein concentration of 20-25 mg/ml. The composition of the reservoir solution was 17% (w/v) PEG10000, 0.1 M ammonium acetate and 0.1 M BIS-TRIS buffer, pH 5.5.
Resolution: 2.99→82.57 Å / Cor.coef. Fo:Fc: 0.933 / Cor.coef. Fo:Fc free: 0.893 / SU B: 18.35 / SU ML: 0.323 / Cross valid method: THROUGHOUT / ESU R: 3.049 / ESU R Free: 0.412 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.25945
963
12.6 %
RANDOM
Rwork
0.19761
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obs
0.20583
6695
93.6 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å