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Yorodumi- PDB-6gw8: Zn(II) form of shortened metallothionein from Pseudomonas fluores... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6gw8 | |||||||||
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Title | Zn(II) form of shortened metallothionein from Pseudomonas fluorescens Q2-87 (residues 1-52) | |||||||||
Components | metallothionein | |||||||||
Keywords | METAL BINDING PROTEIN / metallothionein / zinc(II) ion | |||||||||
Function / homology | Metallothionein, family 14, prokaryote / Prokaryotic metallothionein / Metallothionein domain superfamily / cadmium ion binding / zinc ion binding / Metallothionein Function and homology information | |||||||||
Biological species | Pseudomonas fluorescens Q2-87 (bacteria) | |||||||||
Method | SOLUTION NMR / simulated annealing | |||||||||
Authors | Habjanic, J. / Zerbe, O. / Freisinger, E. | |||||||||
Funding support | Switzerland, 2items
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Citation | Journal: Metallomics / Year: 2018 Title: A histidine-rich Pseudomonas metallothionein with a disordered tail displays higher binding capacity for cadmium than zinc. Authors: Habjanic, J. / Zerbe, O. / Freisinger, E. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6gw8.cif.gz | 291.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6gw8.ent.gz | 243.1 KB | Display | PDB format |
PDBx/mmJSON format | 6gw8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6gw8_validation.pdf.gz | 535 KB | Display | wwPDB validaton report |
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Full document | 6gw8_full_validation.pdf.gz | 692.1 KB | Display | |
Data in XML | 6gw8_validation.xml.gz | 32.4 KB | Display | |
Data in CIF | 6gw8_validation.cif.gz | 39.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gw/6gw8 ftp://data.pdbj.org/pub/pdb/validation_reports/gw/6gw8 | HTTPS FTP |
-Related structure data
Related structure data | 6grvC 6gv6C 6gv7C C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 5767.326 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas fluorescens Q2-87 (bacteria) Gene: PflQ2_2045 / Plasmid: pGEX-4T-1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: J2EKT7 |
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#2: Chemical |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution Contents: 0.5 mM [U-13C; U-15N] metallothionein, 1.5 mM ZINC ION, 50 mM [U-2H] TRIS, 50 mM sodium chloride, 90% H2O/10% D2O Label: 13C_15N_sample / Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 50 mM / Label: conditions_1 / pH: 7.4 / PH err: 0.2 / Pressure: 1 atm / Temperature: 300 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 6 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 20 / Conformers submitted total number: 20 |