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- PDB-6ghl: cyanobacterial GAPDH with full-length CP12 -

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Basic information

Entry
Database: PDB / ID: 6ghl
Titlecyanobacterial GAPDH with full-length CP12
Components
  • CP12 polypeptide
  • Glyceraldehyde-3-phosphate dehydrogenaseGlyceraldehyde 3-phosphate dehydrogenase
KeywordsPHOTOSYNTHESIS / Calvin Cycle / Regulation
Function / homology
Function and homology information


Oxidoreductases; Acting on the aldehyde or oxo group of donors; With NAD+ or NADP+ as acceptor / oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor / glucose metabolic process / NAD binding / NADP binding / nucleotide binding
Similarity search - Function
Calvin cycle protein CP12-like / CP12 domain / CP12 domain / CP12 / Glyceraldehyde-3-phosphate dehydrogenase, type I / Glyceraldehyde 3-phosphate dehydrogenase, active site / Glyceraldehyde 3-phosphate dehydrogenase active site. / Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain / Glyceraldehyde 3-phosphate dehydrogenase, NAD(P) binding domain / Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain ...Calvin cycle protein CP12-like / CP12 domain / CP12 domain / CP12 / Glyceraldehyde-3-phosphate dehydrogenase, type I / Glyceraldehyde 3-phosphate dehydrogenase, active site / Glyceraldehyde 3-phosphate dehydrogenase active site. / Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain / Glyceraldehyde 3-phosphate dehydrogenase, NAD(P) binding domain / Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain / Glyceraldehyde/Erythrose phosphate dehydrogenase family / Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain / Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain / Dihydrodipicolinate Reductase; domain 2 / Dihydrodipicolinate Reductase; domain 2 / NAD(P)-binding domain superfamily / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
MALONATE ION / NICOTINAMIDE-ADENINE-DINUCLEOTIDE / CP12 polypeptide / Glyceraldehyde-3-phosphate dehydrogenase
Similarity search - Component
Biological speciesThermosynechococcus elongatus (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.378 Å
AuthorsMcFarlane, C.R. / Murray, J.W.
Funding support United Kingdom, 1items
OrganizationGrant numberCountry
Biotechnology and Biological Sciences Research CouncilBB/J014575/1 United Kingdom
CitationJournal: Proc Natl Acad Sci U S A / Year: 2019
Title: Structural basis of light-induced redox regulation in the Calvin-Benson cycle in cyanobacteria.
Authors: Ciaran R McFarlane / Nita R Shah / Burak V Kabasakal / Blanca Echeverria / Charles A R Cotton / Doryen Bubeck / James W Murray /
Abstract: Plants, algae, and cyanobacteria fix carbon dioxide to organic carbon with the Calvin-Benson (CB) cycle. Phosphoribulokinase (PRK) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) are essential ...Plants, algae, and cyanobacteria fix carbon dioxide to organic carbon with the Calvin-Benson (CB) cycle. Phosphoribulokinase (PRK) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) are essential CB-cycle enzymes that control substrate availability for the carboxylation enzyme Rubisco. PRK consumes ATP to produce the Rubisco substrate ribulose bisphosphate (RuBP). GAPDH catalyzes the reduction step of the CB cycle with NADPH to produce the sugar glyceraldehyde 3-phosphate (GAP), which is used for regeneration of RuBP and is the main exit point of the cycle. GAPDH and PRK are coregulated by the redox state of a conditionally disordered protein CP12, which forms a ternary complex with both enzymes. However, the structural basis of CB-cycle regulation by CP12 is unknown. Here, we show how CP12 modulates the activity of both GAPDH and PRK. Using thermophilic cyanobacterial homologs, we solve crystal structures of GAPDH with different cofactors and CP12 bound, and the ternary GAPDH-CP12-PRK complex by electron cryo-microscopy, we reveal that formation of the N-terminal disulfide preorders CP12 prior to binding the PRK active site, which is resolved in complex with CP12. We find that CP12 binding to GAPDH influences substrate accessibility of all GAPDH active sites in the binary and ternary inhibited complexes. Our structural and biochemical data explain how CP12 integrates responses from both redox state and nicotinamide dinucleotide availability to regulate carbon fixation.
History
DepositionMay 8, 2018Deposition site: PDBE / Processing site: PDBE
Revision 1.0May 8, 2019Provider: repository / Type: Initial release
Revision 1.1Nov 20, 2019Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Jan 17, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
F: CP12 polypeptide
E: CP12 polypeptide
A: Glyceraldehyde-3-phosphate dehydrogenase
B: Glyceraldehyde-3-phosphate dehydrogenase
C: Glyceraldehyde-3-phosphate dehydrogenase
D: Glyceraldehyde-3-phosphate dehydrogenase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)167,14911
Polymers164,3936
Non-polymers2,7565
Water2,324129
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area24640 Å2
ΔGint-150 kcal/mol
Surface area46560 Å2
MethodPISA
Unit cell
Length a, b, c (Å)80.790, 138.801, 139.772
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein CP12 polypeptide


Mass: 8611.127 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Thermosynechococcus elongatus (strain BP-1) (bacteria)
Strain: BP-1 / Gene: cp12 / Plasmid: pRSETA
Details (production host): modified with thrombin cleavable his-tag
Production host: Escherichia coli (E. coli) / Strain (production host): KRX / References: UniProt: Q8DHX3
#2: Protein
Glyceraldehyde-3-phosphate dehydrogenase / Glyceraldehyde 3-phosphate dehydrogenase


Mass: 36792.734 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Thermosynechococcus elongatus (strain BP-1) (bacteria)
Strain: BP-1 / Gene: tll1466 / Plasmid: pRSETA
Details (production host): modified with thrombin-cleavable his-tag
Production host: Escherichia coli (E. coli) / Strain (production host): KRX
References: UniProt: Q8DIW5, Oxidoreductases; Acting on the aldehyde or oxo group of donors; With NAD+ or NADP+ as acceptor
#3: Chemical
ChemComp-NAD / NICOTINAMIDE-ADENINE-DINUCLEOTIDE / Nicotinamide adenine dinucleotide


Mass: 663.425 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C21H27N7O14P2 / Comment: NAD*YM
#4: Chemical ChemComp-MLI / MALONATE ION / Malonic acid


Mass: 102.046 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C3H2O4
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 129 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.44 Å3/Da / Density % sol: 49.57 %
Crystal growTemperature: 290 K / Method: vapor diffusion / pH: 7 / Details: 100 mM Hepes pH7.0 16% PEG3350 4% Tacsimate

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.97625 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: May 3, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97625 Å / Relative weight: 1
ReflectionResolution: 2.378→40.4 Å / Num. obs: 63755 / % possible obs: 99.76 % / Redundancy: 6.4 % / Biso Wilson estimate: 40.54 Å2 / CC1/2: 0.994 / Rmerge(I) obs: 0.1687 / Rrim(I) all: 0.1838 / Net I/σ(I): 6.82
Reflection shellResolution: 2.378→2.463 Å / Rmerge(I) obs: 0.8387 / Num. unique obs: 6186 / CC1/2: 0.467 / Rrim(I) all: 0.9138 / % possible all: 98.45

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Processing

Software
NameVersionClassification
PHENIX(1.13_2998: ???)refinement
xia2data reduction
xia2data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4BOY

4boy


Resolution: 2.378→40.4 Å / SU ML: 0.32 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 25.89
RfactorNum. reflection% reflection
Rfree0.2468 3148 4.94 %
Rwork0.1969 --
obs0.1995 63724 99.78 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 2.378→40.4 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11186 0 7 129 11322
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00411448
X-RAY DIFFRACTIONf_angle_d0.69715595
X-RAY DIFFRACTIONf_dihedral_angle_d15.3476823
X-RAY DIFFRACTIONf_chiral_restr0.0481781
X-RAY DIFFRACTIONf_plane_restr0.0042005
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.3782-2.41540.33751450.31012596X-RAY DIFFRACTION97
2.4154-2.4550.33821450.30992751X-RAY DIFFRACTION100
2.455-2.49730.38131360.28652712X-RAY DIFFRACTION100
2.4973-2.54270.33271630.28752696X-RAY DIFFRACTION100
2.5427-2.59160.33291110.27772768X-RAY DIFFRACTION100
2.5916-2.64450.33271250.25992731X-RAY DIFFRACTION100
2.6445-2.7020.30471540.24842728X-RAY DIFFRACTION100
2.702-2.76490.28931310.24922730X-RAY DIFFRACTION100
2.7649-2.8340.29361320.25612739X-RAY DIFFRACTION100
2.834-2.91060.33881600.26432714X-RAY DIFFRACTION100
2.9106-2.99620.32081510.2572741X-RAY DIFFRACTION100
2.9962-3.09290.2511200.22012755X-RAY DIFFRACTION100
3.0929-3.20340.26691070.22882758X-RAY DIFFRACTION100
3.2034-3.33170.28241620.21832743X-RAY DIFFRACTION100
3.3317-3.48320.25721700.20262738X-RAY DIFFRACTION100
3.4832-3.66680.20551450.1852768X-RAY DIFFRACTION100
3.6668-3.89640.22911750.17162710X-RAY DIFFRACTION100
3.8964-4.1970.22041370.14752785X-RAY DIFFRACTION100
4.197-4.6190.16651320.13282814X-RAY DIFFRACTION100
4.619-5.28640.20831320.14352810X-RAY DIFFRACTION100
5.2864-6.65650.23711100.17512884X-RAY DIFFRACTION100
6.6565-43.93050.20282050.16362905X-RAY DIFFRACTION100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
17.01542.97-1.94549.14126.99358.3202-0.5953-0.13550.2658-0.0547-0.34612.31450.47650.34980.88041.3460.0904-0.2141.01790.08730.9561-18.025435.7858-9.6602
23.59163.284-1.18765.5685-1.54133.6133-0.10640.50931.48120.49590.32031.6455-0.11940.0029-0.47230.9898-0.16810.1491.44260.27671.41-23.83129.2597-13.4019
34.3471-4.3433.39394.7894-4.7846.92740.62650.90730.0409-1.6119-0.077-0.03870.21950.9604-0.50170.88940.02240.07750.8002-0.00710.6147-8.07938.24026.1834
49.8478-5.0011-4.15682.71583.1417.7828-0.37552.26962.7648-0.5491-0.33223.02-1.013-2.10580.6681.11620.1144-0.25970.97180.01541.5896-7.7244-31.626246.2076
54.515-4.9163.50525.9447-4.93185.6685-0.1633-1.0262-0.85460.91071.9198-0.49171.8623-0.0661-1.64481.00780.0736-0.2350.8205-0.10011.3594-15.1005-25.773440.2339
60.6884-0.61370.10534.4120.45842.092-0.1072-0.2321-0.00740.3710.0452-0.20940.18890.20940.07260.19790.0183-0.02460.42370.00250.23763.9491-14.614255.4684
70.8297-0.11540.54021.34640.21031.81250.0054-0.08310.06380.32180.0127-0.0807-0.08240.1649-0.01670.3163-0.02840.01180.33-0.01250.3003-2.21365.017450.4451
82.35690.0612-1.34322.77061.10662.98020.01620.38940.2492-0.5803-0.0341-0.6042-0.16830.4211-0.05240.34880.00540.15450.53320.10570.467813.89254.498712.9015
90.9409-0.46580.00732.23870.48641.15420.17640.24180.0307-0.3671-0.0809-0.2350.29620.228-0.09650.37430.04270.05410.34410.020.25241.3655-15.840313.9452
101.2345-0.78940.66146.7393-1.37344.178-0.06920.1052-0.007-0.41370.02380.3447-0.0523-0.38670.04970.21960.0084-0.0370.3054-0.02730.2911-26.744515.334713.9476
111.0569-1.2745-1.53132.2533-0.13858.7326-0.10650.02510.4544-0.0570.0293-0.1264-0.7590.0120.06340.3812-0.0578-0.05470.2215-0.04210.4484-20.450229.85924.7029
121.9568-2.423-1.66263.55752.10293.2930.0710.10810.304-0.1311-0.0017-0.1779-0.43620.1175-0.07930.2889-0.05-0.01170.2166-0.00660.2633-11.6516.48737.4425
133.73562.44573.40523.19373.98388.3430.0215-0.71640.49770.7057-0.22940.5421-0.2718-0.55930.20810.51650.04090.11230.241-0.04190.3543-24.036317.809252.8473
140.1956-0.19820.36881.0268-0.54485.8324-0.0409-0.04960.07260.18050.08380.1966-0.3191-0.3354-0.0450.2329-0.01260.05560.3174-0.04490.3115-26.130114.570639.2322
151.82450.6299-0.682.92580.35782.6146-0.0923-0.122-0.29120.2024-0.03720.32780.4521-0.5310.10240.3373-0.15580.0820.45440.02720.5418-33.6238-21.839439.0112
160.9863-0.19520.17971.34460.41681.38250.1454-0.0196-0.1954-0.2178-0.17210.34370.4118-0.17910.03170.3973-0.0434-0.05380.25470.00940.3599-21.155-21.687919.0595
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'F' and (resid 2 through 27 )
2X-RAY DIFFRACTION2chain 'F' and (resid 28 through 52 )
3X-RAY DIFFRACTION3chain 'F' and (resid 53 through 75 )
4X-RAY DIFFRACTION4chain 'E' and (resid 55 through 61 )
5X-RAY DIFFRACTION5chain 'E' and (resid 62 through 75 )
6X-RAY DIFFRACTION6chain 'A' and (resid 0 through 170 )
7X-RAY DIFFRACTION7chain 'A' and (resid 171 through 337 )
8X-RAY DIFFRACTION8chain 'B' and (resid 0 through 153 )
9X-RAY DIFFRACTION9chain 'B' and (resid 154 through 337 )
10X-RAY DIFFRACTION10chain 'C' and (resid 0 through 98 )
11X-RAY DIFFRACTION11chain 'C' and (resid 99 through 153 )
12X-RAY DIFFRACTION12chain 'C' and (resid 154 through 241 )
13X-RAY DIFFRACTION13chain 'C' and (resid 242 through 268 )
14X-RAY DIFFRACTION14chain 'C' and (resid 269 through 337 )
15X-RAY DIFFRACTION15chain 'D' and (resid -1 through 141 )
16X-RAY DIFFRACTION16chain 'D' and (resid 142 through 337 )

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