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Yorodumi- PDB-6g49: Crystal structure of the periplasmic domain of TgpA from Pseudomo... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6g49 | ||||||
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Title | Crystal structure of the periplasmic domain of TgpA from Pseudomonas aeruginosa | ||||||
Components | Protein-glutamine gamma-glutamyltransferaseTransglutaminase | ||||||
Keywords | TRANSFERASE / essential bacterial protein | ||||||
Function / homology | Function and homology information protein-glutamine gamma-glutamyltransferase / protein-glutamine gamma-glutamyltransferase activity / plasma membrane Similarity search - Function | ||||||
Biological species | Pseudomonas aeruginosa PAO1 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.6 Å | ||||||
Authors | Milani, M. / Mastrangelo, E. / Uruburu, M. | ||||||
Citation | Journal: J.Struct.Biol. / Year: 2019 Title: Structural and functional characterization of TgpA, a critical protein for the viability of Pseudomonas aeruginosa. Authors: Uruburu, M. / Mastrangelo, E. / Bolognesi, M. / Ferrara, S. / Bertoni, G. / Milani, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6g49.cif.gz | 85.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6g49.ent.gz | 62.7 KB | Display | PDB format |
PDBx/mmJSON format | 6g49.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/g4/6g49 ftp://data.pdbj.org/pub/pdb/validation_reports/g4/6g49 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 38442.125 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: protein TAG: MGSDKIHHHHHHHHHHGV / Source: (gene. exp.) Pseudomonas aeruginosa PAO1 / Gene: tgpA, PA2873 / Production host: Escherichia coli (E. coli) References: UniProt: Q9HZX3, protein-glutamine gamma-glutamyltransferase | ||||
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#2: Chemical | ChemComp-PO4 / #3: Chemical | ChemComp-CL / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.74 Å3/Da / Density % sol: 55.13 % |
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Crystal grow | Temperature: 293 K / Method: batch mode / pH: 7.5 Details: 0.1 M HEPES sodium pH 7.5 0.8 M Sodium phosphate monobasic monohydrate 0.8 M Potassium phosphate monobasic |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: MASSIF-3 / Wavelength: 0.9677 Å |
Detector | Type: DECTRIS EIGER R 4M / Detector: PIXEL / Date: Apr 1, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9677 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→43.5 Å / Num. all: 429906 / Num. obs: 57370 / % possible obs: 99.9 % / Redundancy: 7.5 % / CC1/2: 0.999 / Rpim(I) all: 0.032 / Rrim(I) all: 0.064 / Net I/σ(I): 17.6 |
Reflection shell | Resolution: 1.6→1.63 Å / Mean I/σ(I) obs: 1.66 / Num. unique obs: 4156 / CC1/2: 0.739 / Rpim(I) all: 0.682 / Rrim(I) all: 1.468 |
-Processing
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.6→43.5 Å / Cor.coef. Fo:Fc: 0.974 / Cor.coef. Fo:Fc free: 0.958 / Cross valid method: THROUGHOUT / ESU R: 0.067 / ESU R Free: 0.072 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.278 Å2
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Refinement step | Cycle: 1 / Resolution: 1.6→43.5 Å
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