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- PDB-6fxn: Crystal structure of human BAFF in complex with Fab fragment of a... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6fxn | |||||||||
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Title | Crystal structure of human BAFF in complex with Fab fragment of anti-BAFF antibody belimumab | |||||||||
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![]() | IMMUNE SYSTEM / Immunology / B cell / cytokine / BAFF / antibody / Proteros | |||||||||
Function / homology | ![]() positive regulation of germinal center formation / B cell costimulation / TNFs bind their physiological receptors / TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway / transitional one stage B cell differentiation / germinal center formation / tumor necrosis factor receptor binding / skin development / B cell proliferation / B cell homeostasis ...positive regulation of germinal center formation / B cell costimulation / TNFs bind their physiological receptors / TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway / transitional one stage B cell differentiation / germinal center formation / tumor necrosis factor receptor binding / skin development / B cell proliferation / B cell homeostasis / T cell proliferation / positive regulation of T cell proliferation / positive regulation of B cell proliferation / tumor necrosis factor-mediated signaling pathway / T cell costimulation / cytokine activity / TNFR2 non-canonical NF-kB pathway / receptor ligand activity / intracellular membrane-bounded organelle / focal adhesion / signaling receptor binding / perinuclear region of cytoplasm / signal transduction / extracellular space / extracellular region / membrane / plasma membrane / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Lammens, A. / Maskos, K. / Willen, L. / Jiang, X. / Schneider, P. | |||||||||
![]() | ![]() Title: A loop region of BAFF controls B cell survival and regulates recognition by different inhibitors. Authors: Vigolo, M. / Chambers, M.G. / Willen, L. / Chevalley, D. / Maskos, K. / Lammens, A. / Tardivel, A. / Das, D. / Kowalczyk-Quintas, C. / Schuepbach-Mallepell, S. / Smulski, C.R. / Eslami, M. / ...Authors: Vigolo, M. / Chambers, M.G. / Willen, L. / Chevalley, D. / Maskos, K. / Lammens, A. / Tardivel, A. / Das, D. / Kowalczyk-Quintas, C. / Schuepbach-Mallepell, S. / Smulski, C.R. / Eslami, M. / Rolink, A. / Hummler, E. / Samy, E. / Fomekong Nanfack, Y. / Mackay, F. / Liao, M. / Hess, H. / Jiang, X. / Schneider, P. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.3 MB | Display | ![]() |
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PDB format | ![]() | 1.1 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 518.7 KB | Display | ![]() |
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Full document | ![]() | 524 KB | Display | |
Data in XML | ![]() | 108.2 KB | Display | |
Data in CIF | ![]() | 152.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18394.908 Da / Num. of mol.: 6 / Mutation: H218A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: TNFSF13B, BAFF, BLYS, TALL1, TNFSF20, ZTNF4, UNQ401/PRO738 Production host: ![]() ![]() #2: Antibody | Mass: 23704.645 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Antibody | Mass: 22764.932 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.26 Å3/Da / Density % sol: 62.29 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / Details: 9% (w/v) PEG4000, 0.1 M MgCl2, 0.1 M HEPES pH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Apr 21, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→138.11 Å / Num. obs: 109365 / % possible obs: 98.7 % / Redundancy: 3.36 % / Net I/σ(I): 11.24 |
Reflection shell | Resolution: 2.9→3.15 Å / Mean I/σ(I) obs: 3.05 / Num. unique obs: 23126 / % possible all: 95.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1KD7 , 7FAB Resolution: 2.9→138.11 Å / Cor.coef. Fo:Fc: 0.934 / Cor.coef. Fo:Fc free: 0.907 / SU B: 31.652 / SU ML: 0.269 / Cross valid method: THROUGHOUT / ESU R: 1.124 / ESU R Free: 0.328 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 59.251 Å2
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Refinement step | Cycle: 1 / Resolution: 2.9→138.11 Å
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Refine LS restraints |
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