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- PDB-6fu2: ATP phosphoribosyltransferase (HisZG ATPPRT) from Psychrobacter a... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6fu2 | ||||||
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Title | ATP phosphoribosyltransferase (HisZG ATPPRT) from Psychrobacter arcticus in complex with PRPP and ATP | ||||||
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![]() | TRANSFERASE / ATP phosphoribosyltransferase / HisZG / cold-adapted | ||||||
Function / homology | ![]() ATP phosphoribosyltransferase / ATP phosphoribosyltransferase activity / histidine-tRNA ligase activity / histidyl-tRNA aminoacylation / L-histidine biosynthetic process / ATP binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Alphey, M.S. / Ge, Y. / Fisher, G. / Czekster, C.M. / Naismith, J.H. / da Silva, R.G. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Catalytic and Anticatalytic Snapshots of a Short-Form ATP Phosphoribosyltransferase Authors: Alphey, M.S. / Fisher, G. / Hirschi, J.S. / Stroek, R. / Ge, Y. / Gould, E.R. / Czekster, C.M. / Liu, H. / Florence, G.J. / Vetticatt, M.J. / Naismith, J.H. / da Silva, R.G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 241.3 KB | Display | ![]() |
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PDB format | ![]() | 190.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.6 MB | Display | ![]() |
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Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 39.6 KB | Display | |
Data in CIF | ![]() | 54.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6fcaC ![]() 6fccC ![]() 6fctC ![]() 6fcwC ![]() 6fcyC ![]() 6fd9C ![]() 6fttC ![]() 6fu7C ![]() 6fuaC ![]() 5m8hS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: _ / Refine code: _
NCS ensembles :
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Components
-Protein , 2 types, 4 molecules ABCD
#1: Protein | Mass: 43129.039 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Missing sections are due to flexible regions not visible in the electron density maps. N-terminal Gly remains after tag cleavage. Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 25240.965 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Missing sections are due to flexible regions not visible in the electron density maps. N-terminal Gly remains after tag cleavage. Source: (gene. exp.) ![]() ![]() ![]() |
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-Sugars , 1 types, 2 molecules ![](data/chem/img/PRP.gif)
#4: Sugar |
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-Non-polymers , 4 types, 16 molecules ![](data/chem/img/TRS.gif)
![](data/chem/img/ATP.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/ATP.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | #5: Chemical | #6: Chemical | #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.54 Å3/Da / Density % sol: 51.51 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 8mg mL-1 protein (in 20mM Tris pH7, 50mM KCl, 10mM MgCl2, 2mM DTT, 0.5mM histidine) mixed in 1:1 ratio with precipitant solution (11% PEG3350, 100mM bicine pH8.5, 150mM SrCl2, 150mM KBr, 2% 1,6-hexanediol) |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Apr 27, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9686 Å / Relative weight: 1 |
Reflection | Resolution: 2.71→73.11 Å / Num. obs: 36859 / % possible obs: 99.6 % / Redundancy: 4.1 % / Biso Wilson estimate: 64.8 Å2 / CC1/2: 0.996 / Rmerge(I) obs: 0.093 / Rpim(I) all: 0.052 / Rrim(I) all: 0.107 / Net I/σ(I): 10.8 |
Reflection shell | Resolution: 2.71→2.78 Å / Redundancy: 4.2 % / Rmerge(I) obs: 1.095 / Num. unique obs: 2720 / CC1/2: 0.581 / Rpim(I) all: 0.59 / Rrim(I) all: 1.248 / % possible all: 99.7 |
-Phasing
Phasing | Method: ![]() |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: 5M8H Resolution: 2.71→39.05 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.932 / SU B: 16.129 / SU ML: 0.302 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.344 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.7 Å / Shrinkage radii: 0.7 Å / VDW probe radii: 1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 171.71 Å2 / Biso mean: 80.589 Å2 / Biso min: 47.55 Å2
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Refinement step | Cycle: final / Resolution: 2.71→39.05 Å
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Refine LS restraints |
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Refine LS restraints NCS | Refine-ID: X-RAY DIFFRACTION / Type: interatomic distance / Rms dev position: 0.09 Å / Weight position: 0.05
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LS refinement shell | Resolution: 2.71→2.78 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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