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- PDB-6ffu: Solution NMR structure of CBM64 from S.thermophila using 20% 13C,... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6ffu | |||||||||
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Title | Solution NMR structure of CBM64 from S.thermophila using 20% 13C, 100% 15N | |||||||||
![]() | Glycosyl hydrolase family 5 cellulase CBM64 | |||||||||
![]() | SUGAR BINDING PROTEIN / CBM64 | |||||||||
Function / homology | ![]() cellulase / metabolic process / hydrolase activity, hydrolyzing O-glycosyl compounds Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | SOLUTION NMR / simulated annealing | |||||||||
![]() | Heikkinen, H.A. / Iwai, H. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: NMR Structure Determinations of Small Proteins Using only One Fractionally 20% 13 C- and Uniformly 100% 15 N-Labeled Sample. Authors: Heikkinen, H.A. / Backlund, S.M. / Iwai, H. | |||||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 514.9 KB | Display | ![]() |
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PDB format | ![]() | 440 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 472.3 KB | Display | ![]() |
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Full document | ![]() | 618.4 KB | Display | |
Data in XML | ![]() | 32.4 KB | Display | |
Data in CIF | ![]() | 53.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6ffqC ![]() 6sowC C: citing same article ( |
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Similar structure data | |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 10025.756 Da / Num. of mol.: 1 Fragment: C-terminal 86-residue module of CBM64 from S.thermophila, UNP residues 456-541 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution Contents: 9.85 mg/mL 20% 13C, 100% 15N CBM64_20C13, 95% H2O/5% D2O Details: NMR sample / Label: 13C_20%_15N_100% / Solvent system: 95% H2O/5% D2O |
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Sample | Conc.: 9.85 mg/mL / Component: CBM64_20C13 / Isotopic labeling: 20% 13C, 100% 15N |
Sample conditions | Ionic strength: 20 mM / Label: conditions_20 / pH: 6.0 / Pressure: 1 bar / Temperature: 303 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 850 MHz / Details: HD |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 4 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 20 / Conformers submitted total number: 20 |