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- PDB-6fbz: Crystal structure of the eIF4E-eIF4G complex from Chaetomium ther... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6fbz | ||||||
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Title | Crystal structure of the eIF4E-eIF4G complex from Chaetomium thermophilum in the cap-bound state | ||||||
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![]() | TRANSLATION / Translation Translational control Translation initiation Gene expression | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gruener, S. / Valkov, E. | ||||||
![]() | ![]() Title: Structural motifs in eIF4G and 4E-BPs modulate their binding to eIF4E to regulate translation initiation in yeast. Authors: Gruner, S. / Weber, R. / Peter, D. / Chung, M.Y. / Igreja, C. / Valkov, E. / Izaurralde, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 176.8 KB | Display | ![]() |
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PDB format | ![]() | 142.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6fc0C ![]() 6fc1C ![]() 6fc2C ![]() 6fc3C ![]() 4tpwS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 23081.289 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: CTHT_0058450 / Production host: ![]() ![]() |
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#2: Protein | Mass: 9048.340 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: CTHT_0071550 / Production host: ![]() ![]() |
#3: Chemical | ChemComp-MGP / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.82 Å3/Da / Density % sol: 32.28 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop Details: 10% w/v PEG 20000 20% PEG 500 MME 0.02 M 1,6-hexanediol 0.02 M 1-butanol 0.02 M (RS)-1,2-propanediol 0.02 M 2-propanol 0.02 M 1,4-butanediol 0.02 M 1,3-propanediol 0.1 M Bicine/Trizma pH 8.5 0.687 mM m7GpppG |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 20, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→75.35 Å / Num. obs: 38441 / % possible obs: 99.6 % / Redundancy: 12.7 % / Rsym value: 0.074 / Net I/σ(I): 16 |
Reflection shell | Resolution: 1.5→1.52 Å / Redundancy: 10.7 % / Mean I/σ(I) obs: 1.2 / Num. unique obs: 1742 / CC1/2: 0.416 / Rsym value: 1.913 / % possible all: 91.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4TPW Resolution: 1.496→48.763 Å / SU ML: 0.18 / Cross valid method: FREE R-VALUE / σ(F): 1.23 / Phase error: 20.94
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.496→48.763 Å
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Refine LS restraints |
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LS refinement shell |
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