+Open data
-Basic information
Entry | Database: PDB / ID: 6eyq | ||||||
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Title | Crystal structure of a mutated OpuBC in complex with choline | ||||||
Components | Choline-binding protein | ||||||
Keywords | TRANSPORT PROTEIN / substrate binding protein | ||||||
Function / homology | Function and homology information amino acid transport / transmembrane transporter activity / ATP-binding cassette (ABC) transporter complex Similarity search - Function | ||||||
Biological species | Bacillus subtilis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.5 Å | ||||||
Authors | Peherstorfer, S. / Teichmann, L. / Smits, S.H. / Schmitt, L. / Bremer, E. | ||||||
Citation | Journal: To Be Published Title: Crystal structure of a mutated OpuBC in complex with choline Authors: Peherstorfer, S. / Teichmann, L. / Smits, S.H. / Schmitt, L. / Bremer, E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6eyq.cif.gz | 233.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6eyq.ent.gz | 188.9 KB | Display | PDB format |
PDBx/mmJSON format | 6eyq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ey/6eyq ftp://data.pdbj.org/pub/pdb/validation_reports/ey/6eyq | HTTPS FTP |
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-Related structure data
Related structure data | 3r6uS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 34430.543 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: WE mutated D74 to Threonine Source: (gene. exp.) Bacillus subtilis (strain 168) (bacteria) Strain: 168 / Gene: opuBC, proX, BSU33710 / Production host: Escherichia coli (E. coli) / References: UniProt: Q45462 #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal grow | Temperature: 273 K / Method: batch mode / pH: 5 / Details: PEG3350 phosphatte buffer / PH range: 4-6 |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-1 / Wavelength: 0.973 Å |
Detector | Type: MAR CCD 130 mm / Detector: CCD / Date: Nov 18, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.973 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→42.1 Å / Num. obs: 77957 / % possible obs: 99.3 % / Redundancy: 6.5 % / Rsym value: 0.048 / Net I/σ(I): 25.6 |
Reflection shell | Resolution: 1.5→1.55 Å / Redundancy: 5.4 % / Mean I/σ(I) obs: 12 / Num. unique obs: 7783 / Rsym value: 0.14 / % possible all: 99.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3R6U Resolution: 1.5→42.1 Å / SU ML: 0.09 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 17.43
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.5→42.1 Å
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Refine LS restraints |
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LS refinement shell |
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