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Open data
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Basic information
Entry | Database: PDB / ID: 6exx | ||||||
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Title | Crystal Structure of Pes4 RRM4 | ||||||
![]() | Protein PES4 | ||||||
![]() | RNA BINDING PROTEIN / RNA Recognition Motif | ||||||
Function / homology | ![]() mRNA localization resulting in post-transcriptional regulation of gene expression / mRNA metabolic process / prospore membrane / poly(A) binding / poly(U) RNA binding / Translation initiation complex formation / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / L13a-mediated translational silencing of Ceruloplasmin expression / mRNA 3'-UTR binding ...mRNA localization resulting in post-transcriptional regulation of gene expression / mRNA metabolic process / prospore membrane / poly(A) binding / poly(U) RNA binding / Translation initiation complex formation / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / L13a-mediated translational silencing of Ceruloplasmin expression / mRNA 3'-UTR binding / cytoplasmic stress granule / regulation of translation / ribonucleoprotein complex / RNA binding / nucleus / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Mohamad, N. / Bravo, J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal Structure of Pes4 RRM4 at 1.1 Angstroms resolution Authors: Mohamad, N. / Bravo, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 60.2 KB | Display | ![]() |
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PDB format | ![]() | 43.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 394.2 KB | Display | ![]() |
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Full document | ![]() | 394.2 KB | Display | |
Data in XML | ![]() | 6.2 KB | Display | |
Data in CIF | ![]() | 8.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 9243.413 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 204508 / S288c / Gene: PES4, YFR023W / Production host: ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.98 Å3/Da / Density % sol: 37.85 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop Details: 0.2 M Ammonium Nitrate 20% PEG 3350 0.1 M Betaine Monohydrate (as an additive) |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 15, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97921 Å / Relative weight: 1 |
Reflection | Resolution: 1.1→25.79 Å / Num. obs: 30587 / % possible obs: 99.8 % / Redundancy: 12.3 % / Rmerge(I) obs: 0.05842 / Rrim(I) all: 0.06103 / Net I/σ(I): 21.51 |
Reflection shell | Resolution: 1.1→1.12 Å / Rmerge(I) obs: 0.6737 / % possible all: 100 |
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Processing
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Refinement | Resolution: 1.1→25.79 Å / SU ML: 0.08 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 14.79
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.1→25.79 Å
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Refine LS restraints |
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LS refinement shell |
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