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- PDB-6exp: Crystal structure of the SIRV3 AcrID1 (gp02) anti-CRISPR protein -

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Basic information

Entry
Database: PDB / ID: 6exp
TitleCrystal structure of the SIRV3 AcrID1 (gp02) anti-CRISPR protein
ComponentsSIRV3 AcrID1 (gp02) anti-CRISPR protein
KeywordsVIRAL PROTEIN / Anti-CRISPR / CRISPR-Cas / Sulfolobus islandicus rod shaped virus 2 / inhibitor / SIRV3
Function / homologySulfolobus islandicus filamentous virus, Orf14 / Protein of unknown function (DUF1374) / Uncharacterized protein
Function and homology information
Biological speciesSulfolobus islandicus rudivirus 3
MethodX-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 1.93 Å
AuthorsHe, F. / Bhoobalan-Chitty, Y. / Van, L.B. / Kjeldsen, A.L. / Dedola, M. / Makarova, K.S. / Koonin, E.V. / Brodersen, D.E. / Peng, X.
Funding support Denmark, 1items
OrganizationGrant numberCountry
Danish National Research FoundationDNRF120 Denmark
CitationJournal: Nat Microbiol / Year: 2018
Title: Anti-CRISPR proteins encoded by archaeal lytic viruses inhibit subtype I-D immunity.
Authors: He, F. / Bhoobalan-Chitty, Y. / Van, L.B. / Kjeldsen, A.L. / Dedola, M. / Makarova, K.S. / Koonin, E.V. / Brodersen, D.E. / Peng, X.
History
DepositionNov 8, 2017Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jan 31, 2018Provider: repository / Type: Initial release
Revision 1.1May 16, 2018Group: Data collection / Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2May 8, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: SIRV3 AcrID1 (gp02) anti-CRISPR protein
B: SIRV3 AcrID1 (gp02) anti-CRISPR protein
C: SIRV3 AcrID1 (gp02) anti-CRISPR protein
D: SIRV3 AcrID1 (gp02) anti-CRISPR protein
E: SIRV3 AcrID1 (gp02) anti-CRISPR protein
F: SIRV3 AcrID1 (gp02) anti-CRISPR protein


Theoretical massNumber of molelcules
Total (without water)78,5206
Polymers78,5206
Non-polymers00
Water1,02757
1
A: SIRV3 AcrID1 (gp02) anti-CRISPR protein
B: SIRV3 AcrID1 (gp02) anti-CRISPR protein


Theoretical massNumber of molelcules
Total (without water)26,1732
Polymers26,1732
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1900 Å2
ΔGint-13 kcal/mol
Surface area11290 Å2
MethodPISA
2
C: SIRV3 AcrID1 (gp02) anti-CRISPR protein
D: SIRV3 AcrID1 (gp02) anti-CRISPR protein


Theoretical massNumber of molelcules
Total (without water)26,1732
Polymers26,1732
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1930 Å2
ΔGint-14 kcal/mol
Surface area11370 Å2
MethodPISA
3
E: SIRV3 AcrID1 (gp02) anti-CRISPR protein
F: SIRV3 AcrID1 (gp02) anti-CRISPR protein


Theoretical massNumber of molelcules
Total (without water)26,1732
Polymers26,1732
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1900 Å2
ΔGint-13 kcal/mol
Surface area11350 Å2
MethodPISA
Unit cell
Length a, b, c (Å)54.750, 80.440, 106.320
Angle α, β, γ (deg.)90.000, 101.230, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21
31
41
51
61

NCS domain segments:

Ens-ID: 1 / Beg auth comp-ID: MET / Beg label comp-ID: MET / End auth comp-ID: LYS / End label comp-ID: LYS / Auth seq-ID: 1 - 96 / Label seq-ID: 1 - 96

Dom-IDComponent-IDSelection detailsAuth asym-IDLabel asym-ID
11chain AAA
22(chain B and resid 1 through 96)BB
33chain CCC
44(chain D and resid 1 through 96)DD
55chain EEE
66(chain F and resid 1 through 96)FF

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Components

#1: Protein
SIRV3 AcrID1 (gp02) anti-CRISPR protein


Mass: 13086.693 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfolobus islandicus rudivirus 3 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Rosetta2 / References: UniProt: A0A1B3SN05
#2: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 57 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.1 Å3/Da / Density % sol: 60.33 %
Crystal growTemperature: 292 K / Method: vapor diffusion, sitting drop / pH: 8.5
Details: 18-20% (w/v) PEG 8000, 0.2 M MgCl2, 0.1 M Tris-Cl, pH 8.5

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P13 (MX1) / Wavelength: 0.9802 Å
DetectorType: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Aug 31, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9802 Å / Relative weight: 1
ReflectionResolution: 1.93→26.89 Å / Num. obs: 67149 / % possible obs: 98.7 % / Redundancy: 3.4 % / Biso Wilson estimate: 48.09 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.036 / Rrim(I) all: 0.043 / Net I/σ(I): 13.2
Reflection shellResolution: 1.93→1.96 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.978 / Mean I/σ(I) obs: 1.2 / Num. unique obs: 3320 / CC1/2: 0.598 / Rrim(I) all: 1.17 / % possible all: 97.8

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Processing

Software
NameVersionClassification
XDSdata reduction
XSCALEdata scaling
SHELXDphasing
Cootmodel building
PHENIX1.12_2829refinement
RefinementMethod to determine structure: SAD / Resolution: 1.93→26.89 Å / SU ML: 0.268688593713 / Cross valid method: FREE R-VALUE / σ(F): 1.33786083638 / Phase error: 34.3761580119
Details: Iterative reciprocal space refinement with manual rebuilding
RfactorNum. reflection% reflectionSelection details
Rfree0.269017141927 3482 5.18880576998 %RANDOM
Rwork0.234673721848 ---
obs0.236476965072 67106 98.5982956215 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 80.9142368216 Å2
Refinement stepCycle: LAST / Resolution: 1.93→26.89 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5235 0 0 57 5292
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01303830153235376
X-RAY DIFFRACTIONf_angle_d1.20587170967242
X-RAY DIFFRACTIONf_chiral_restr0.072211485127732
X-RAY DIFFRACTIONf_plane_restr0.00847094921318927
X-RAY DIFFRACTIONf_dihedral_angle_d20.12198260493186
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.93-1.95650.3761205631651360.4073857410782505X-RAY DIFFRACTION97.6340110906
1.9565-1.98440.395471908921300.3881493441062518X-RAY DIFFRACTION98.3655274889
1.9844-2.0140.4031187864621570.3405492570822548X-RAY DIFFRACTION99.3024963289
2.014-2.04550.3067659817081380.313417478072510X-RAY DIFFRACTION98.7322893363
2.0455-2.0790.3228492757911250.3134344829692565X-RAY DIFFRACTION99.3719985223
2.079-2.11480.3276975571151360.3069327861212613X-RAY DIFFRACTION99.6375498369
2.1148-2.15330.3187904606651380.2901799238762502X-RAY DIFFRACTION98.8393859978
2.1533-2.19470.3237885497161320.2798159021072537X-RAY DIFFRACTION97.8372434018
2.1947-2.23940.3092534354651420.2699890818792490X-RAY DIFFRACTION98.0625931446
2.2394-2.28810.2937931577751570.2857649568482526X-RAY DIFFRACTION97.8126139264
2.2881-2.34130.3207525500451200.2666831472112500X-RAY DIFFRACTION97.5428145942
2.3413-2.39980.3487060604571310.2716656868492539X-RAY DIFFRACTION98.0896399706
2.3998-2.46470.3392271301751230.2746937109432555X-RAY DIFFRACTION98.8556662975
2.4647-2.53710.3104046103121650.2713614329182527X-RAY DIFFRACTION99.2259491338
2.5371-2.6190.3089326171061450.2815070132472577X-RAY DIFFRACTION99.1982507289
2.619-2.71250.3128487599371180.275638118982551X-RAY DIFFRACTION98.5234403839
2.7125-2.8210.3290031166971490.2726151661432546X-RAY DIFFRACTION99.6303142329
2.821-2.94920.3013576520511530.2656629725572582X-RAY DIFFRACTION99.3822674419
2.9492-3.10450.3019064741671370.2477446193292569X-RAY DIFFRACTION99.4487320838
3.1045-3.29870.2982506135131430.2476437391852531X-RAY DIFFRACTION98.6715867159
3.2987-3.55280.2523997789871340.2152635659572569X-RAY DIFFRACTION98.7216946676
3.5528-3.90940.2273084622951710.1947340579042547X-RAY DIFFRACTION98.7645348837
3.9094-4.47290.2239934982281500.1735600442922553X-RAY DIFFRACTION98.8299817185
4.4729-5.62680.208003135431460.1858726743752507X-RAY DIFFRACTION96.4376590331
5.6268-26.89270.2885588190771060.2541686054522657X-RAY DIFFRACTION98.1178977273
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
15.41854416391.05560831510.2914770395643.099188177941.383728450597.48966327081-0.2311677638260.272935893125-0.371410601326-0.4068541455590.359937722861-0.6802440076740.1166165006980.424386036943-0.1581666029510.4774264786130.007336801024640.0254296869550.31308638983-0.1055731920750.453154976734-11.88816068072.32134300906-47.3405169393
29.333841634610.5043941165143.490731746563.887164195941.663043441968.80344705585-0.262732565132-0.0331033195002-0.2910799381990.3162498802740.556655272339-0.905354761655-0.05933066190540.68072104737-0.3613475966540.6776650400610.139311350656-0.01193041327750.462318649547-0.08523284151790.65012311563-3.07855770633-6.53024160066-42.2445117132
34.0718154381-1.374938812241.357295037516.49759961551-0.7103083947743.46119340040.0015860388799-0.0665678698663-0.4177045278461.003654661360.114595692991-0.7174252095990.7138381183980.271841191479-0.0154505444290.6784539054680.0367349907052-0.1215052190040.304164167032-0.02785831450180.447494775916-10.3484483782-0.785744013496-34.0901274601
45.76256599411-0.08258323962412.317838485596.05149915784-0.8472454243975.715755045720.133451810783-0.303140200751-0.4224594614720.2523854095130.2334762206910.2109281574070.5648950660370.32767050373-0.5529368429880.560070783123-0.02291878707780.002817331465970.319328405643-0.003852746341880.317681556544-14.2720091708-0.755991567978-37.727882651
55.80640311396-1.99375723751.615690076845.90494311453-3.963384190584.9874563649-0.09722824936430.5694691355470.0625505595807-1.079271579450.292473286752-0.446309256459-0.3621410018610.46563896635-0.293805398540.780351902324-0.1456147827590.1247147770350.551545667851-0.1026369429390.395823556062-13.91899282070.335637834485-56.327892305
66.42361545067-0.59098190145-0.9145751224172.36880220996-0.4793340719898.50136715078-0.04641172465930.4138755388010.3271782701070.383876740630.3534991026720.402824572547-0.693410332568-0.237461418176-0.3775123367570.564107811394-0.114277532378-0.04801094869940.419311609261-0.02035957020590.526897212413-24.66684192565.24396334919-51.8674809062
73.041726046581.709890001734.181806722166.143617874524.298878226966.56576260835-0.651318126020.3506425512340.559459777608-2.530658427440.35820420801-0.518161624627-0.876687505841.622900303641.084035971870.840540543961-0.183081422067-0.04811456188280.7150267346640.1135585885590.623820532226-22.2917683842.6046690957-66.1932433548
86.52997650602-1.75951913904-4.578256090542.774909355923.875603731098.028857973230.751020829689-0.144075426514-0.729122501411-0.341788651481-0.9654933851970.729362933296-0.382825310786-1.077917949090.1079221486420.616797675964-0.08468394926710.06021317567870.814446982799-0.1363403366890.662816135449-35.5307664517-2.5892561283-58.8671812523
95.00300036989-2.49062443369-1.142545276065.402823163573.802531859284.815293640630.02115537793730.1457115921090.255568552258-0.510087456964-0.3666090002580.374033221486-0.47840138293-0.5095117146040.3329809547280.476279882543-0.0163561219911-0.04717286360250.481790053484-0.06264121979140.48152123854-25.3247699363-4.41301733009-59.9382331381
107.38626868254-7.017391991331.026676186259.49250535547-0.5765757965655.90036203894-0.360984385258-0.0198756048380.578644704964-0.2860366764030.103108879921-0.102780169961-0.18326376171-0.3141480954670.004473330585620.494329388252-0.0796410850452-0.02952017736370.389243903049-0.0457539990830.44855407766-24.910085212-1.9920975239-54.5920918327
114.446915347211.157724957261.849714121853.419501400173.245073726213.248762174280.751855014414-1.527653752311.434310489711.403933724430.1445577716871.008431256221.72031036325-1.10514749457-0.4042693055050.938764699245-0.1140998670180.05459608215360.5281873737210.1631378903790.653203113387-26.3947937933-27.101279541-59.6627382113
129.772323418245.00969945238-2.16065464962.09750269727-4.805305014612.60928794712-0.1555220303-0.27176999118-0.06925258775990.2843605661040.2505296550561.19576035513-0.570950875672-0.284915299474-0.1844288448450.6333450973580.04408607008490.05483160949260.6526384189230.08251667877990.700853114385-10.02272053245.8374527176-1.04654378552
134.88904937309-0.2661959447060.638845004328.61680363638-2.878819390546.367217107850.485378444038-0.18053608023-0.5307272466020.146368735692-0.06730077376170.634851211720.0845965366786-0.61762014777-0.3441187286480.46470462716-0.00341021060368-0.02278177299160.4498995533050.07647386242120.328293057676-8.658812976490.908830910577-6.88028365872
143.18525473931.818954412651.964088069348.52201477917-0.08742140042645.015629247970.1988608033-0.284115481227-0.367575081523-0.6060991661940.1732778245220.5802611679570.528020605196-0.554789365233-0.2260966010020.47394992924-0.00134065728976-0.0631249365160.3864109355490.07263266452590.426602280645-9.202469168582.91921031782-16.2007193377
155.188244339570.555844339234-0.7366349697476.81764456559-0.3585069387653.59987064238-0.1630030283050.211030280318-0.621663675869-0.5084944138320.529599836811-0.233390139290.255189187847-0.607351409336-0.4947702601480.602768333805-0.0984494741855-0.09588850656880.3971143863750.05230216853570.345863306352-4.692460711152.71933527879-13.1956536831
166.66349450740.09091488604890.06643407340113.952484512834.281334365675.31875123415-0.135255677962-0.7855172981990.5118620816750.3144044912660.4364485108650.522926227787-0.755498058434-0.0484776603671-0.3210302234430.738632167378-0.0498700489253-0.01817426993910.5352621716860.08709596052340.43077081856-2.283002692254.40185096925.06359261081
179.305710713670.4705967722322.264362251746.863076111660.2130420201167.370530698110.4293052995730.6136490595740.2076262179870.47902917038-0.277984693996-1.05300391539-0.4657211829370.553140678736-0.3589587391090.50534905629-0.0480938681061-0.07012965113250.4824143968910.1788513755830.5173231059926.449302479055.65133798603-4.55109548827
188.22520123382-4.704692735742.980168446834.44465031823-0.8739529869554.60271360336-0.465957849432-0.3917516801560.6095452768740.240549555564-0.246195658129-0.171993974621-1.175265607120.478311708870.846593843470.761585980181-0.118691883488-0.1790888538820.6548543659110.1009322900960.72691279010912.86148988054.909370331578.5815888021
196.847878085992.21222384191-2.216394352185.83280845331-3.417739010274.76071514344-0.2146274179230.05701930114980.8238604269880.2894743917-0.208306305708-0.170000160595-0.5896047178090.774444787990.2158146534930.501288832597-0.0832031547222-0.1624043032810.4527979025870.1066905232170.4684700067619.36791369064-1.279615791197.18729071458
206.086169407425.29984974398-1.79245313718.8194461229-2.787629490234.249311389-0.4254259567070.5102004020430.8105265389830.8792322728190.0523195283383-0.895699089794-0.8457535670020.3095500296910.1057130753020.535036049417-0.0994973559174-0.1782011358730.4778307904490.2475518907910.7007783989518.22729444551.266176217211.90398379836
211.567782467861.90728532492-0.3121652506182.73052156823-1.075553880523.615491179450.1542732601220.5449354044470.488778738518-0.743013995403-0.889577644509-1.837024444150.3332195484241.00427401688-0.1104654599940.703915974947-0.08088401274540.1164007383550.5815201310070.05988875152910.7714703060959.04864142994-22.12644535636.21578978372
224.94304069225-1.15462530345-3.275078775644.52395737874-1.698427092328.43764687567-0.1226139613060.458709473571-0.492916301885-0.2824952402560.230304923842-0.06704059047030.98531424634-0.252417445801-0.2213547670990.41281509687-0.0398925450119-0.08159356137970.504945043507-0.004321945241390.522448206295-37.68472309622.9322705925-29.5713438482
235.82905907024-3.19594604571-1.071718002387.904045605280.469947662027.30429373333-0.1873162063450.88983249708-0.44329627049-0.08084330281880.5123239493330.2003189642380.842690065507-1.01261875135-0.003682729205560.572231390946-0.149998715521-0.09078142770950.8051511713530.001989186974390.445431371828-45.894883156824.4344841213-40.7528803572
245.972388109690.751138508525-4.815929856413.08812775702-0.8256119160737.792007926440.2667737862530.8547372860850.265719567798-0.155773992873-0.179099373242-0.0579100709218-0.167581356426-0.08471874600290.03188262017280.360267151990.00268924873343-0.05121954407550.7383127842910.105651370250.449454405485-45.539270832229.7585880356-36.0353805932
253.153678081171.35579986109-3.635492154553.42391136410.706712842967.80356191160.5926648128620.476316557383-0.262727411358-0.0459376182659-0.4858387272370.0617556319418-1.039879826530.330253225799-0.2707681281680.364937621891-0.00670129169746-0.01374395836310.5885175624360.1093617439260.566755811726-43.320083423630.4984809075-32.4567801199
267.02810561871.606314898-7.517460832323.18824739582-2.894168691559.03633353474-0.517732757064-0.55548423108-0.4493500212970.6069519670750.228893296723-0.2533611739341.052465032820.1678403929190.4249877481040.84216863104-0.0194357348791-0.1185417855970.7638432507520.1745596508060.82484541074-32.926798704117.8787217176-21.215593705
273.7769193068-1.74148860257-2.104134835224.578911624880.7401212627031.511552961550.135906873279-0.438523021016-0.1599734129240.116942750232-0.02012454578580.1581843557250.0494289696835-0.481361629730.01923605202840.412962238702-0.042184480684-0.1134507424160.752146736372-0.03822349166640.49163471285-33.771029197327.0334030859-21.2426475544
284.962317300614.24103525937-3.819139369363.86086694428-3.158983155292.83243633520.373934642722-1.58669441291-0.5181577975680.8470656176710.0598822248528-0.254821739350.3119593259860.383491022278-0.3978630936670.8459457924480.0108369259568-0.233686636580.9530038578360.07855375841450.731215853209-22.760618575322.0215105862-12.7994948541
294.90821462043-1.57304397409-2.182854428587.403859390924.107903773913.487710620920.0772471559214-0.6687636209620.0995090482150.104456925422-0.0460661017360.1383070269140.208728406583-0.023995677712-0.3477944015090.4612589339990.158963889011-0.1480953508260.775720539177-0.1088722931310.478614359628-21.600318106729.9162657147-17.2953259042
306.19349968845-0.7555494355-0.3700404667932.311990679971.154466299832.061258417670.371254938596-0.8747768180580.1727858949310.482505244954-0.0399715404821-0.27585122864-0.01601308257610.182303699667-0.1442683674350.4810615389390.0254183232099-0.1088147428850.693249036384-0.1141700712020.531001240595-25.329765175329.3166049384-17.6991256294
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 25 )
2X-RAY DIFFRACTION2chain 'A' and (resid 26 through 40 )
3X-RAY DIFFRACTION3chain 'A' and (resid 41 through 81 )
4X-RAY DIFFRACTION4chain 'A' and (resid 82 through 96 )
5X-RAY DIFFRACTION5chain 'B' and (resid 1 through 20 )
6X-RAY DIFFRACTION6chain 'B' and (resid 21 through 34 )
7X-RAY DIFFRACTION7chain 'B' and (resid 35 through 44 )
8X-RAY DIFFRACTION8chain 'B' and (resid 45 through 55 )
9X-RAY DIFFRACTION9chain 'B' and (resid 56 through 81 )
10X-RAY DIFFRACTION10chain 'B' and (resid 82 through 94 )
11X-RAY DIFFRACTION11chain 'B' and (resid 95 through 100 )
12X-RAY DIFFRACTION12chain 'C' and (resid 1 through 15 )
13X-RAY DIFFRACTION13chain 'C' and (resid 16 through 40 )
14X-RAY DIFFRACTION14chain 'C' and (resid 41 through 81 )
15X-RAY DIFFRACTION15chain 'C' and (resid 82 through 96 )
16X-RAY DIFFRACTION16chain 'D' and (resid 1 through 20 )
17X-RAY DIFFRACTION17chain 'D' and (resid 21 through 29 )
18X-RAY DIFFRACTION18chain 'D' and (resid 30 through 55 )
19X-RAY DIFFRACTION19chain 'D' and (resid 56 through 81 )
20X-RAY DIFFRACTION20chain 'D' and (resid 82 through 94 )
21X-RAY DIFFRACTION21chain 'D' and (resid 95 through 99 )
22X-RAY DIFFRACTION22chain 'E' and (resid 1 through 29 )
23X-RAY DIFFRACTION23chain 'E' and (resid 30 through 51 )
24X-RAY DIFFRACTION24chain 'E' and (resid 52 through 81 )
25X-RAY DIFFRACTION25chain 'E' and (resid 82 through 96 )
26X-RAY DIFFRACTION26chain 'F' and (resid 1 through 15 )
27X-RAY DIFFRACTION27chain 'F' and (resid 16 through 33 )
28X-RAY DIFFRACTION28chain 'F' and (resid 34 through 48 )
29X-RAY DIFFRACTION29chain 'F' and (resid 49 through 67 )
30X-RAY DIFFRACTION30chain 'F' and (resid 68 through 99 )

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