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- PDB-6ekj: Crystal structure of mammalian Rev7 in complex with human Chromos... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6ekj | ||||||
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Title | Crystal structure of mammalian Rev7 in complex with human Chromosome alignment-maintaining phosphoprotein 1 | ||||||
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![]() | REPLICATION / Rev7 / Mad2L2 / DNA replication / Chromosome Alignment Maintaining Phosphoprotein 1 / Champ1 | ||||||
Function / homology | ![]() Translesion synthesis by REV1 / Translesion synthesis by POLK / Translesion synthesis by POLI / somatic diversification of immunoglobulins involved in immune response / DNA damage response, signal transduction resulting in transcription / sister chromatid biorientation / zeta DNA polymerase complex / protein localization to microtubule / telomere maintenance in response to DNA damage / anaphase-promoting complex ...Translesion synthesis by REV1 / Translesion synthesis by POLK / Translesion synthesis by POLI / somatic diversification of immunoglobulins involved in immune response / DNA damage response, signal transduction resulting in transcription / sister chromatid biorientation / zeta DNA polymerase complex / protein localization to microtubule / telomere maintenance in response to DNA damage / anaphase-promoting complex / positive regulation of isotype switching / positive regulation of extracellular matrix assembly / negative regulation of transcription by competitive promoter binding / negative regulation of cell-cell adhesion mediated by cadherin / attachment of mitotic spindle microtubules to kinetochore / JUN kinase binding / protein localization to kinetochore / negative regulation of epithelial to mesenchymal transition / negative regulation of ubiquitin protein ligase activity / condensed chromosome / positive regulation of double-strand break repair via nonhomologous end joining / negative regulation of double-strand break repair via homologous recombination / error-prone translesion synthesis / positive regulation of epithelial to mesenchymal transition / actin filament organization / negative regulation of protein catabolic process / regulation of cell growth / negative regulation of canonical Wnt signaling pathway / kinetochore / spindle / positive regulation of peptidyl-serine phosphorylation / double-strand break repair / site of double-strand break / RNA polymerase II-specific DNA-binding transcription factor binding / nuclear body / cell division / positive regulation of gene expression / positive regulation of DNA-templated transcription / chromatin / negative regulation of transcription by RNA polymerase II / nucleoplasm / nucleus / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Huber, F. / Tropia, L. / Emamzadah, S. / Halazonetis, T. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of mammalian Rev7 in complex with Champ1 - CAPS molecule Authors: Huber, F. / Tropia, L. / Emamzadah, S. / Halazonetis, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 60.6 KB | Display | ![]() |
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PDB format | ![]() | 42.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5o8kS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 24473.545 Da / Num. of mol.: 1 / Mutation: F11S, G12A, V132K, C133V, A135K Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 2934.389 Da / Num. of mol.: 1 / Fragment: 328-355 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#3: Chemical | ChemComp-CXS / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.8 Å3/Da / Density % sol: 56.13 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 10.5 Details: 1.8 M ammonium sulfate, 0.09 M CAPS/sodium hydroxide pH 10.5, 0.18 M lithium sulfate, 0.009 M sodium nitrate, 0.009 M sodium phosphate dibasic, 0.01 M Tris Bicine pH 8.5, 1.25% v/v MPD, 1. ...Details: 1.8 M ammonium sulfate, 0.09 M CAPS/sodium hydroxide pH 10.5, 0.18 M lithium sulfate, 0.009 M sodium nitrate, 0.009 M sodium phosphate dibasic, 0.01 M Tris Bicine pH 8.5, 1.25% v/v MPD, 1.25% PEG 1000, 1.25% w/v PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Jun 25, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.00002 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→70 Å / Num. obs: 41580 / % possible obs: 100 % / Redundancy: 11.96 % / Rsym value: 0.055 / Net I/σ(I): 26.8 |
Reflection shell | Resolution: 1.6→1.69 Å / Num. unique obs: 5973 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5O8K Resolution: 1.6→21.49 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 1.6→21.49 Å
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