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Open data
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Basic information
Entry | Database: PDB / ID: 6.0E+16 | ||||||
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Title | Ternary structure of c-Myc-TBP-TAF1 | ||||||
![]() | Transcription initiation factor TFIID subunit 1,Myc proto-oncogene protein,TATA-box-binding protein | ||||||
![]() | DNA BINDING PROTEIN / transcriptional regulation / carcinogenesis | ||||||
Function / homology | ![]() SCF ubiquitin ligase complex binding / positive regulation of metanephric cap mesenchymal cell proliferation / negative regulation of transcription initiation by RNA polymerase II / Myc-Max complex / RNA polymerase II transcription repressor complex / regulation of cell cycle process / regulation of somatic stem cell population maintenance / Binding of TCF/LEF:CTNNB1 to target gene promoters / RUNX3 regulates WNT signaling / TFIIA-class transcription factor complex binding ...SCF ubiquitin ligase complex binding / positive regulation of metanephric cap mesenchymal cell proliferation / negative regulation of transcription initiation by RNA polymerase II / Myc-Max complex / RNA polymerase II transcription repressor complex / regulation of cell cycle process / regulation of somatic stem cell population maintenance / Binding of TCF/LEF:CTNNB1 to target gene promoters / RUNX3 regulates WNT signaling / TFIIA-class transcription factor complex binding / TFAP2 (AP-2) family regulates transcription of cell cycle factors / RNA polymerase III transcription regulatory region sequence-specific DNA binding / transcription factor TFIIIB complex / RNA polymerase III preinitiation complex assembly / negative regulation of cell division / regulation of transcription by RNA polymerase III / RNA polymerase I general transcription initiation factor binding / negative regulation of monocyte differentiation / transcription regulator activator activity / response to growth factor / Transcription of E2F targets under negative control by DREAM complex / transcription factor TFIIA complex / RNA polymerase I preinitiation complex assembly / negative regulation of stress-activated MAPK cascade / fibroblast apoptotic process / DNA binding, bending / regulation of telomere maintenance / Regulation of NFE2L2 gene expression / positive regulation of mesenchymal cell proliferation / protein-DNA complex disassembly / RNA Polymerase III Transcription Initiation From Type 2 Promoter / negative regulation of gene expression via chromosomal CpG island methylation / branching involved in ureteric bud morphogenesis / RNA polymerase II transcribes snRNA genes / Signaling by ALK / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / transcription factor TFIID complex / RNA Polymerase II Pre-transcription Events / RNA polymerase II general transcription initiation factor activity / RNA Polymerase I Promoter Escape / E-box binding / nucleolar large rRNA transcription by RNA polymerase I / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / positive regulation of cysteine-type endopeptidase activity involved in apoptotic process / Estrogen-dependent gene expression / : / chromosome organization / RNA polymerase II core promoter sequence-specific DNA binding / Cyclin E associated events during G1/S transition / core promoter sequence-specific DNA binding / Cyclin A:Cdk2-associated events at S phase entry / negative regulation of fibroblast proliferation / histone acetyltransferase activity / RNA polymerase II preinitiation complex assembly / histone acetyltransferase / ERK1 and ERK2 cascade / TBP-class protein binding / positive regulation of epithelial cell proliferation / DNA-templated transcription initiation / transcription coregulator binding / response to gamma radiation / SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription / MAPK6/MAPK4 signaling / NOTCH1 Intracellular Domain Regulates Transcription / DNA-binding transcription repressor activity, RNA polymerase II-specific / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / positive regulation of miRNA transcription / Transcriptional regulation of granulopoiesis / G1/S transition of mitotic cell cycle / cellular response to UV / disordered domain specific binding / MAPK cascade / positive regulation of fibroblast proliferation / cellular response to xenobiotic stimulus / cellular response to hypoxia / regulation of gene expression / DNA-binding transcription activator activity, RNA polymerase II-specific / Interleukin-4 and Interleukin-13 signaling / DNA-binding transcription factor binding / intracellular iron ion homeostasis / Estrogen-dependent gene expression / RNA polymerase II-specific DNA-binding transcription factor binding / transcription regulator complex / transcription by RNA polymerase II / molecular adaptor activity / protein dimerization activity / DNA-binding transcription factor activity, RNA polymerase II-specific / Ub-specific processing proteases / response to xenobiotic stimulus / chromatin remodeling / protein heterodimerization activity / RNA polymerase II cis-regulatory region sequence-specific DNA binding / negative regulation of DNA-templated transcription / DNA damage response / chromatin binding / positive regulation of cell population proliferation Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Wei, Y. / Dong, A. / Sunnerhagen, M. / Penn, L. / Tong, Y. / Edwards, A.M. / Arrowsmith, C.H. / Structural Genomics Consortium (SGC) | ||||||
![]() | ![]() Title: Multiple direct interactions of TBP with the MYC oncoprotein. Authors: Wei, Y. / Resetca, D. / Li, Z. / Johansson-Akhe, I. / Ahlner, A. / Helander, S. / Wallenhammar, A. / Morad, V. / Raught, B. / Wallner, B. / Kokubo, T. / Tong, Y. / Penn, L.Z. / Sunnerhagen, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 100.4 KB | Display | ![]() |
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PDB format | ![]() | 75.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6e24C ![]() 40baS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 28509.570 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() Strain: ATCC 204508 / S288c Gene: TAF1, TAF130, TAF145, YGR274C, G9374, MYC, BHLHE39, SPT15, BTF1, TBP1, YER148W Production host: ![]() ![]() References: UniProt: P46677, UniProt: P01106, UniProt: P13393, histone acetyltransferase |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.66 Å3/Da / Density % sol: 66.44 % |
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Crystal grow | Temperature: 291 K / Method: batch mode Details: 20% (w/w) PEG8000, 0.2M ammonium sulfare, and 0.1M HEPES pH7.5 |
-Data collection
Diffraction | Mean temperature: 80 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Mar 20, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97934 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→36.408 Å / Num. obs: 16091 / % possible obs: 99.4 % / Redundancy: 3.7 % / Net I/σ(I): 13.75 |
Reflection shell | Resolution: 2.4→2.51 Å |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 40BA Resolution: 2.4→36.408 Å / SU ML: 0.34 / Cross valid method: THROUGHOUT / σ(F): 1.39 / Phase error: 23.63
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 131.73 Å2 / Biso mean: 56.2219 Å2 / Biso min: 28.38 Å2 | ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.4→36.408 Å
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Refinement TLS params. | Method: refined / Origin x: 100.4981 Å / Origin y: 76.6317 Å / Origin z: -11.1363 Å
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Refinement TLS group |
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