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- PDB-6dz7: afTMEM16 reconstituted in nanodiscs in the absence of Ca2+ -

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Basic information

Entry
Database: PDB / ID: 6dz7
TitleafTMEM16 reconstituted in nanodiscs in the absence of Ca2+
ComponentsPlasma membrane channel protein (Aqy1), putative
KeywordsLIPID TRANSPORT / scramblase / Ca2+-activated / membrane-reorganization
Function / homologyCalcium-activated chloride channel / Anoctamin / phospholipid scramblase activity / phospholipid translocation / voltage-gated calcium channel activity / ion transmembrane transport / integral component of membrane / Plasma membrane channel protein (Aqy1), putative
Function and homology information
Specimen sourceNeosartorya fumigata (mold)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / 3.89 Å resolution
AuthorsFalzone, M.E. / Accardi, A.
CitationJournal: Elife / Year: 2019
Title: Structural basis of Ca-dependent activation and lipid transport by a TMEM16 scramblase.
Authors: Maria E Falzone / Jan Rheinberger / Byoung-Cheol Lee / Thasin Peyear / Linda Sasset / Ashleigh M Raczkowski / Edward T Eng / Annarita Di Lorenzo / Olaf S Andersen / Crina M Nimigean / Alessio Accardi
Abstract: The lipid distribution of plasma membranes of eukaryotic cells is asymmetric and phospholipid scramblases disrupt this asymmetry by mediating the rapid, nonselective transport of lipids down their ...The lipid distribution of plasma membranes of eukaryotic cells is asymmetric and phospholipid scramblases disrupt this asymmetry by mediating the rapid, nonselective transport of lipids down their concentration gradients. As a result, phosphatidylserine is exposed to the outer leaflet of membrane, an important step in extracellular signaling networks controlling processes such as apoptosis, blood coagulation, membrane fusion and repair. Several TMEM16 family members have been identified as Ca-activated scramblases, but the mechanisms underlying their Ca-dependent gating and their effects on the surrounding lipid bilayer remain poorly understood. Here, we describe three high-resolution cryo-electron microscopy structures of a fungal scramblase from , afTMEM16, reconstituted in lipid nanodiscs. These structures reveal that Ca-dependent activation of the scramblase entails global rearrangement of the transmembrane and cytosolic domains. These structures, together with functional experiments, suggest that activation of the protein thins the membrane near the transport pathway to facilitate rapid transbilayer lipid movement.
Validation Report
SummaryFull reportAbout validation report
DateDeposition: Jul 3, 2018 / Release: Feb 6, 2019

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Assembly

Deposited unit
B: Plasma membrane channel protein (Aqy1), putative
A: Plasma membrane channel protein (Aqy1), putative


Theoretical massNumber of molelcules
Total (without water)169,2342
Polyers169,2342
Non-polymers00
Water0
1


TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein/peptide Plasma membrane channel protein (Aqy1), putative


Mass: 84616.859 Da / Num. of mol.: 2
Source: (gene. exp.) Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (mold)
Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100 / Gene: AFUA_4G02970 / Production host: Saccharomyces cerevisiae (baker's yeast) / References: UniProt: Q4WA18

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / Reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: afTMEM16 reconstituted in nanodiscs in the absence of Ca2+
Type: COMPLEX / Entity ID: 1 / Source: RECOMBINANT
Molecular weightValue: 168 kDa/nm / Experimental value: NO
Source (natural)Organism: Aspergillus fumigatus (mold)
Source (recombinant)Organism: Saccharomyces cerevisiae (baker's yeast)
Buffer solutionpH: 8
SpecimenConc.: 7 mg/ml
Details: afTMEM16 reconstituted in nanodiscs in the absence of Ca2+
Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 288 kelvins

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyMicroscope model: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: OTHER
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 62.61 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Number of grids imaged: 1

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Processing

Software
NameVersionClassification
phenix.real_space_refine1.13_2998refinement
PHENIX1.13_2998refinement
EM software
IDNameVersionCategory
2Leginonimage acquisition
4CTFFIND4CTF correction
9PHENIXmodel refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
SymmetryPoint symmetry: C2
3D reconstructionResolution: 3.89 Å / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 70356 / Symmetry type: POINT
RefineStereochemistry target values: GeoStd + Monomer Library
Refine LS restraints
Refine IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.00969518
ELECTRON MICROSCOPYf_angle_d1.236912936
ELECTRON MICROSCOPYf_chiral_restr0.06621474
ELECTRON MICROSCOPYf_plane_restr0.00901600
ELECTRON MICROSCOPYf_dihedral_angle_d12.41375596

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