+Open data
-Basic information
Entry | Database: PDB / ID: 6dxo | |||||||||
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Title | 1.8 A structure of RsbN-BldN complex. | |||||||||
Components |
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Keywords | TRANSCRIPTION / BldN / RsbN / Sigma / anti-sigma / ECF | |||||||||
Function / homology | Function and homology information sigma factor activity / DNA-templated transcription initiation / membrane => GO:0016020 / DNA binding Similarity search - Function | |||||||||
Biological species | Streptomyces venezuelae (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.8 Å | |||||||||
Authors | Schumacher, M.A. | |||||||||
Citation | Journal: Nucleic Acids Res. / Year: 2018 Title: The crystal structure of the RsbN-sigma BldN complex from Streptomyces venezuelae defines a new structural class of anti-sigma factor. Authors: Schumacher, M.A. / Bush, M.J. / Bibb, M.J. / Ramos-Leon, F. / Chandra, G. / Zeng, W. / Buttner, M.J. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6dxo.cif.gz | 98.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6dxo.ent.gz | 73 KB | Display | PDB format |
PDBx/mmJSON format | 6dxo.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6dxo_validation.pdf.gz | 433.8 KB | Display | wwPDB validaton report |
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Full document | 6dxo_full_validation.pdf.gz | 437.5 KB | Display | |
Data in XML | 6dxo_validation.xml.gz | 11 KB | Display | |
Data in CIF | 6dxo_validation.cif.gz | 14.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dx/6dxo ftp://data.pdbj.org/pub/pdb/validation_reports/dx/6dxo | HTTPS FTP |
-Related structure data
Related structure data | 6c03 S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 17165.463 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745) (bacteria) Strain: ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745 Gene: SVEN_3185 / Production host: Escherichia coli / References: UniProt: F2R911*PLUS |
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#2: Protein | Mass: 9446.593 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745) (bacteria) Strain: ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745 Gene: SVEN_3186 / Production host: Escherichia coli / References: UniProt: F2R912 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.97 Å3/Da / Density % sol: 37.58 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / Details: 0.2 M ammonium nitrate, 26% PEG 3500 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.3.1 / Wavelength: 1.01 Å |
Detector | Type: ADSC QUANTUM 210r / Detector: CCD / Date: Apr 25, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.01 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→23.06 Å / Num. obs: 29209 / % possible obs: 92.8 % / Observed criterion σ(F): 0 / Redundancy: 2.5 % / Biso Wilson estimate: 21 Å2 / CC1/2: 0.996 / Rpim(I) all: 0.044 / Rrim(I) all: 0.075 / Rsym value: 0.06 / Net I/σ(I): 10 |
Reflection shell | Resolution: 1.8→1.91 Å / Redundancy: 2.6 % / Mean I/σ(I) obs: 3.9 / CC1/2: 0.977 / Rpim(I) all: 0.101 / Rrim(I) all: 0.17 / Rsym value: 0.136 / % possible all: 99.3 |
-Phasing
Phasing | Method: molecular replacement |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6C03 6c03 Resolution: 1.8→23.06 Å / FOM work R set: 0.82 / SU ML: 0.24 / Cross valid method: THROUGHOUT / σ(F): 0.77 / Phase error: 24.92 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.72 Å / VDW probe radii: 1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 71.058 Å2 / ksol: 0.453 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 143.16 Å2 / Biso mean: 38.4 Å2 / Biso min: 11.45 Å2
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Refinement step | Cycle: final / Resolution: 1.8→23.06 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 10
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Refinement TLS params. | Method: refined / Origin x: -5.5551 Å / Origin y: 0.569 Å / Origin z: 24.9492 Å
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Refinement TLS group |
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