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Open data
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Basic information
Entry | Database: PDB / ID: 6dsi | |||||||||
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Title | Anti recombinant prolactin receptor scFv | |||||||||
![]() | Anti-TN-C scFv | |||||||||
![]() | IMMUNE SYSTEM / anti prolactin receptor scFV | |||||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta![]() | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() ![]() | |||||||||
![]() | Langley, D.B. / Rouet, R. / Christ, D. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Anti recombinant prolactin receptor scFv Authors: Rouet, R. / Langley, D.B. / Christ, D. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 58.6 KB | Display | ![]() |
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PDB format | ![]() | 40.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6dn0S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 25560.133 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.53 Å3/Da / Density % sol: 65.15 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 9 Details: Equal volumes (2 microlitres) of protein solution (5 mg/mL in 15 mM Tris (pH 7.5) and 50 mM NaCl) were combined with well solution (2% (v/v) Dioxane, 100 mM Bicine (pH 9.0) and 10% (w/v) PEG4000) |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Nov 7, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9537 Å / Relative weight: 1 |
Reflection | Resolution: 2.49→37.8 Å / Num. obs: 12684 / % possible obs: 99.9 % / Redundancy: 6.6 % / CC1/2: 0.972 / Rmerge(I) obs: 0.113 / Rpim(I) all: 0.049 / Rrim(I) all: 0.123 / Net I/σ(I): 10.1 |
Reflection shell | Resolution: 2.49→2.59 Å / Redundancy: 6.5 % / Rmerge(I) obs: 0.513 / Num. unique obs: 1397 / CC1/2: 0.905 / Rpim(I) all: 0.216 / Rrim(I) all: 0.557 / % possible all: 100 |
-Phasing
Phasing | Method: ![]() | |||||||||
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Phasing MR | Model details: Phaser MODE: MR_AUTO
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6dn0 Resolution: 2.49→36.86 Å / Cor.coef. Fo:Fc: 0.922 / Cor.coef. Fo:Fc free: 0.916 / SU B: 8.097 / SU ML: 0.175 / SU R Cruickshank DPI: 0.3087 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.309 / ESU R Free: 0.236 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 116.27 Å2 / Biso mean: 43.716 Å2 / Biso min: 23.03 Å2
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Refinement step | Cycle: final / Resolution: 2.49→36.86 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.488→2.552 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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