The structures represent different time points in the divalent and monovalent metal-dependent hydrolysis reaction of RNA by Ribonuclease H1, which is bound to an RNA/DNA hybrid in the crystal.
Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Mar 14, 2015
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1 Å / Relative weight: 1
Reflection
Resolution: 1.36→19.86 Å / Num. obs: 40176 / % possible obs: 98.2 % / Redundancy: 1 % / Biso Wilson estimate: 17.01 Å2 / Net I/σ(I): 6.6 / Num. measured all: 40176
Reflection shell
Resolution (Å)
Redundancy (%)
Num. unique obs
Diffraction-ID
% possible all
1.36-1.38
1
1616
1
82
7.44-19.86
1
252
1
92.7
-
Processing
Software
Name
Version
Classification
Aimless
0.5.31
datascaling
PHENIX
1.10_2155
refinement
PDB_EXTRACT
3.24
dataextraction
HKL-2000
datareduction
PHENIX
phasing
Refinement
Resolution: 1.36→19.859 Å / SU ML: 0.2 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 21.34 Details: Structures refined in Phenix and nucleic acid and protein residues built in Coot