Evidence: native gel electrophoresis, native gel electrophoresis showed a reduced mobility that saturated at a ratio of 2:1 showing that 2 proteins bind 1 RNA molecule, light scattering, Dynamic ...Evidence: native gel electrophoresis, native gel electrophoresis showed a reduced mobility that saturated at a ratio of 2:1 showing that 2 proteins bind 1 RNA molecule, light scattering, Dynamic light scattering experiments indicated that the RNA-protein complex was the correct molecular weight, indicating that the complex formed a homogeneous sample and did not aggregate.
Type
Name
Symmetry operation
Number
identity operation
1_555
x,y,z
1
Buried area
2630 Å2
ΔGint
-17 kcal/mol
Surface area
17380 Å2
Method
PISA
Unit cell
Length a, b, c (Å)
47.910, 34.030, 95.010
Angle α, β, γ (deg.)
90.000, 95.180, 90.000
Int Tables number
4
Space group name H-M
P1211
-
Components
#1: Protein
La-relatedprotein7 / La ribonucleoprotein domain family member 7 / P-TEFb-interaction protein for 7SK stability / PIP7S
Mass: 13006.630 Da / Num. of mol.: 2 / Fragment: RNA binding domain / Mutation: L475M, E501L, Q504L, L521M Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: LARP7, HDCMA18P / Plasmid: pETXa/LIC / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q4G0J3
#2: RNA chain
human7SKRNAstem-loop4
Mass: 12296.301 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)
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