negative regulation of leukocyte tethering or rolling / cytolysis in another organism / Chemokine receptors bind chemokines / chemokine activity / positive regulation of cell-matrix adhesion / cell chemotaxis / response to nutrient / chemotaxis / antimicrobial humoral immune response mediated by antimicrobial peptide / positive regulation of cytosolic calcium ion concentration ...negative regulation of leukocyte tethering or rolling / cytolysis in another organism / Chemokine receptors bind chemokines / chemokine activity / positive regulation of cell-matrix adhesion / cell chemotaxis / response to nutrient / chemotaxis / antimicrobial humoral immune response mediated by antimicrobial peptide / positive regulation of cytosolic calcium ion concentration / G alpha (i) signalling events / killing of cells of another organism / immune response / G protein-coupled receptor signaling pathway / extracellular space / extracellular exosome / extracellular region Similarity search - Function
CC chemokine, conserved site / Small cytokines (intercrine/chemokine) C-C subfamily signature. / Chemokine interleukin-8-like domain / Chemokine interleukin-8-like superfamily / Small cytokines (intecrine/chemokine), interleukin-8 like Similarity search - Domain/homology
Ionic strength: 15 mM / Label: conditions_1 / pH: 6.2 / Pressure: 1 atm / Temperature: 298 K
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NMR measurement
NMR spectrometer
Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600.13 MHz
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Processing
NMR software
Name
Version
Developer
Classification
TopSpin
2.6
BrukerBiospin
collection
NMRPipe
Delaglio, Grzesiek, Vuister, Zhu, PfeiferandBax
processing
XEASY
Bartelsetal.
dataanalysis
GARANT
Bartels, Guntert, BilleterandWuthrich
chemicalshiftassignment
CYANA
Guntert, MumenthalerandWuthrich
structurecalculation
CYANA
Guntert, MumenthalerandWuthrich
refinement
X-PLOR NIH
Schwieters, Kuszewski, TjandraandClore
geometryoptimization
Refinement
Method: torsion angle dynamics / Software ordinal: 1 Details: Structures are based on a total of 1487 restraints, 1382 NOE-derived distance constraints and 105 dihedral angle constraints
NMR representative
Selection criteria: target function
NMR ensemble
Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20
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