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- PDB-6c8w: Crystal structure of Aspartate Semialdehyde Dehydrogenase with NA... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6c8w | |||||||||
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Title | Crystal structure of Aspartate Semialdehyde Dehydrogenase with NADP from Blastomyces dermatitidis | |||||||||
![]() | Aspartate-semialdehyde dehydrogenase | |||||||||
![]() | OXIDOREDUCTASE / Rossman fold / antifungal inhibitor complex / NADP cofactor | |||||||||
Function / homology | ![]() aspartate-semialdehyde dehydrogenase / aspartate-semialdehyde dehydrogenase activity / threonine biosynthetic process / lysine biosynthetic process via diaminopimelate / NAD binding / NADP binding / protein dimerization activity Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Dahal, G.P. / Viola, R.E. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural insights into inhibitor binding to a fungal ortholog of aspartate semialdehyde dehydrogenase. Authors: Dahal, G.P. / Viola, R.E. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 147.9 KB | Display | ![]() |
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PDB format | ![]() | 115.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 28.2 KB | Display | |
Data in CIF | ![]() | 38.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6c85C ![]() 3hskS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 40152.977 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47.1 % / Description: long rod-shaped |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 0.2M Ammonium Citrate Tribasic pH 7.0, 18%w/v PEG3350 PH range: 6.5-8.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Apr 24, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0332 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→93.06 Å / Num. obs: 46721 / % possible obs: 100 % / Redundancy: 9.7 % / Biso Wilson estimate: 61 Å2 / CC1/2: 0.996 / Net I/σ(I): 9.1 |
Reflection shell | Resolution: 2.6→2.69 Å / Redundancy: 9.7 % / Mean I/σ(I) obs: 2 / Num. unique obs: 46721 / CC1/2: 0.769 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3hsk Resolution: 2.6→47.836 Å / SU ML: 0.33 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 25.39 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||
Displacement parameters | Biso max: 120.69 Å2 / Biso mean: 63.0233 Å2 / Biso min: 37.75 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.6→47.836 Å
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