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- PDB-6c31: Crystal structure of TetR family protein Rv0078 in complex with DNA -
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Open data
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Basic information
Entry | Database: PDB / ID: 6c31 | ||||||
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Title | Crystal structure of TetR family protein Rv0078 in complex with DNA | ||||||
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![]() | DNA BINDING PROTEIN/DNA / Rv0078 / DNA / TetR family / DNA BINDING PROTEIN-DNA complex | ||||||
Function / homology | ![]() transcription cis-regulatory region binding / DNA-binding transcription factor activity / regulation of DNA-templated transcription / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hsu, H.C. / Li, H. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Cytokinin Signaling in Mycobacterium tuberculosis. Authors: Samanovic, M.I. / Hsu, H.C. / Jones, M.B. / Jones, V. / McNeil, M.R. / Becker, S.H. / Jordan, A.T. / Strnad, M. / Xu, C. / Jackson, M. / Li, H. / Darwin, K.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 346.3 KB | Display | ![]() |
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PDB format | ![]() | 275.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 488.6 KB | Display | ![]() |
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Full document | ![]() | 505.4 KB | Display | |
Data in XML | ![]() | 52.5 KB | Display | |
Data in CIF | ![]() | 72.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5wm9SC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 23477.922 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 25618 / H37Rv / Gene: LH57_00450 / Production host: ![]() ![]() #2: DNA chain | Mass: 7049.574 Da / Num. of mol.: 2 / Source method: obtained synthetically Source: (synth.) ![]() #3: DNA chain | Mass: 7071.570 Da / Num. of mol.: 2 / Source method: obtained synthetically Source: (synth.) ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.84 Å3/Da / Density % sol: 56.63 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.2 M magnesium formate dihydrate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Aug 7, 2017 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.12713 Å / Relative weight: 1 |
Reflection | Resolution: 3→28.74 Å / Num. obs: 46330 / % possible obs: 94.8 % / Redundancy: 6.4 % / Rpim(I) all: 0.087 / Net I/σ(I): 5.5 |
Reflection shell | Resolution: 3→3.16 Å / Rpim(I) all: 0.474 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 5WM9 Resolution: 3→28.495 Å / Cross valid method: FREE R-VALUE
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Displacement parameters | Biso mean: 73 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→28.495 Å
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