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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 6bu9 | ||||||
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タイトル | Drosophila Dicer-2 bound to blunt dsRNA | ||||||
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![]() | rna binding protein/rna / Dicer / Dcr2 / Dcr-2 / dmDcr-2 / Dicer-2 / helicase / dsRNA / RNA / RNA BINDING PROTEIN / rna binding protein-rna complex | ||||||
機能・相同性 | ![]() lncRNA catabolic process / : / positive regulation of Toll signaling pathway / dsRNA transport / dosage compensation by hyperactivation of X chromosome / global gene silencing by mRNA cleavage / ribonuclease III / apoptotic DNA fragmentation / RISC-loading complex / deoxyribonuclease I activity ...lncRNA catabolic process / : / positive regulation of Toll signaling pathway / dsRNA transport / dosage compensation by hyperactivation of X chromosome / global gene silencing by mRNA cleavage / ribonuclease III / apoptotic DNA fragmentation / RISC-loading complex / deoxyribonuclease I activity / detection of virus / RISC complex assembly / ribonuclease III activity / regulatory ncRNA-mediated post-transcriptional gene silencing / siRNA binding / ATP-dependent activity, acting on RNA / siRNA processing / positive regulation of innate immune response / RISC complex / heterochromatin formation / positive regulation of defense response to virus by host / helicase activity / locomotory behavior / mRNA 3'-UTR binding / cellular response to virus / cytoplasmic ribonucleoprotein granule / defense response to virus / perinuclear region of cytoplasm / ATP hydrolysis activity / RNA binding / ATP binding / nucleus / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() unidentified (未定義) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 6.8 Å | ||||||
![]() | Shen, P.S. / Sinha, N.K. / Bass, B.L. | ||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Dicer uses distinct modules for recognizing dsRNA termini. 著者: Niladri K Sinha / Janet Iwasa / Peter S Shen / Brenda L Bass / ![]() 要旨: Invertebrates rely on Dicer to cleave viral double-stranded RNA (dsRNA), and Dicer-2 distinguishes dsRNA substrates by their termini. Blunt termini promote processive cleavage, while 3' overhanging ...Invertebrates rely on Dicer to cleave viral double-stranded RNA (dsRNA), and Dicer-2 distinguishes dsRNA substrates by their termini. Blunt termini promote processive cleavage, while 3' overhanging termini are cleaved distributively. To understand this discrimination, we used cryo-electron microscopy to solve structures of Dicer-2 alone and in complex with blunt dsRNA. Whereas the Platform-PAZ domains have been considered the only Dicer domains that bind dsRNA termini, unexpectedly, we found that the helicase domain is required for binding blunt, but not 3' overhanging, termini. We further showed that blunt dsRNA is locally unwound and threaded through the helicase domain in an adenosine triphosphate-dependent manner. Our studies reveal a previously unrecognized mechanism for optimizing antiviral defense and set the stage for the discovery of helicase-dependent functions in other Dicers. | ||||||
履歴 |
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構造の表示
ムービー |
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構造ビューア | 分子: ![]() ![]() |
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ダウンロード
PDBx/mmCIF形式 | ![]() | 121.3 KB | 表示 | ![]() |
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PDB形式 | ![]() | 64 KB | 表示 | ![]() |
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その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 759.7 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 759.3 KB | 表示 | |
XML形式データ | ![]() | 16.1 KB | 表示 | |
CIF形式データ | ![]() | 23.8 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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要素
#1: タンパク質 | 分子量: 198074.797 Da / 分子数: 1 / 由来タイプ: 組換発現 由来: (組換発現) ![]() ![]() 遺伝子: Dcr-2, Dcr-2-RA, CG6493, Dmel_CG6493 発現宿主: ![]() ![]() 参照: UniProt: A1ZAW0, ribonuclease III, EC: 3.6.1.3 |
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#2: RNA鎖 | 分子量: 16781.023 Da / 分子数: 1 / 由来タイプ: 合成 / 由来: (合成) unidentified (未定義) |
#3: RNA鎖 | 分子量: 16528.768 Da / 分子数: 1 / 由来タイプ: 合成 / 由来: (合成) unidentified (未定義) |
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
構成要素 | 名称: Drosophila Dicer-2 bound to blunt dsRNA / タイプ: COMPLEX / Entity ID: all / 由来: RECOMBINANT |
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由来(天然) | 生物種: ![]() ![]() |
由来(組換発現) | 生物種: ![]() ![]() |
緩衝液 | pH: 8 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE |
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電子顕微鏡撮影
顕微鏡 | モデル: FEI TECNAI 20 |
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電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD |
撮影 | 電子線照射量: 1.2 e/Å2 フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) |
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解析
CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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対称性 | 点対称性: C1 (非対称) |
3次元再構成 | 解像度: 6.8 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 19445 / 対称性のタイプ: POINT |