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- PDB-6bu9: Drosophila Dicer-2 bound to blunt dsRNA -

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Database: PDB / ID: 6bu9
TitleDrosophila Dicer-2 bound to blunt dsRNA
DescriptorDicer-2, isoform A/RNA Complex
Keywordsrna binding protein/rna / Dicer / Dcr2 / Dcr-2 / dmDcr-2 / Dicer-2 / helicase / dsRNA / RNA / RNA BINDING PROTEIN / rna binding protein-rna complex
Specimen sourceDrosophila melanogaster / arthropod / Fruit fly / キイロショウジョウバエ /
MethodElectron microscopy (6.8 Å resolution / Particle / Single particle)
AuthorsShen, P.S. / Sinha, N.K. / Bass, B.L.
CitationScience, 2017

Science, 2017 Yorodumi Papers
Dicer uses distinct modules for recognizing dsRNA termini.
Niladri K Sinha / Janet Iwasa / Peter S Shen / Brenda L Bass

Validation Report
SummaryFull reportAbout validation report
DateDeposition: Dec 9, 2017 / Release: Dec 27, 2017
RevisionDateData content typeGroupCategoryItemProviderType
1.0Dec 27, 2017Structure modelrepositoryInitial release
1.1Jan 3, 2018Structure modelDatabase referencescitation_citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
1.2Jan 17, 2018Structure modelAuthor supporting evidencepdbx_audit_support_pdbx_audit_support.funding_organization

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Deposited unit
A: Dicer-2, isoform A

Theoretical massNumber of molelcules
Total (without water)231,3853

TypeNameSymmetry operationNumber
identity operation1_5551


#1: Polypeptide(L)Dicer-2, isoform A / FI15132p1

Mass: 198074.797 Da / Num. of mol.: 1
Source: (gene. exp.) Drosophila melanogaster / arthropod / キイロショウジョウバエ /
References: UniProt: A1ZAW0, EC:, EC:

Mass: 16781.023 Da / Num. of mol.: 1 / Source: (synth.) Unidentified

Mass: 16528.768 Da / Num. of mol.: 1 / Source: (synth.) Unidentified

Experimental details


EM experimentAggregation state: PARTICLE / Reconstruction method: SINGLE PARTICLE

Sample preparation

ComponentName: Drosophila Dicer-2 bound to blunt dsRNA / Type: COMPLEX / Entity ID: 1, 2, 3 / Source: RECOMBINANT
Source (natural)Organism: Drosophila melanogaster
Source (recombinant)Organism: Spodoptera frugiperda
Buffer solutionpH: 8
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

Electron microscopy imaging

MicroscopyMicroscope model: FEI TECNAI 20
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD
Image recordingElectron dose: 1.2 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k)


SymmetryPoint symmetry: C1
3D reconstructionResolution: 6.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 19445 / Symmetry type: POINT

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