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- PDB-6bjj: Human ABO(H) blood group glycosyltransferase GTB D302A mutant -

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Basic information

Entry
Database: PDB / ID: 6bjj
TitleHuman ABO(H) blood group glycosyltransferase GTB D302A mutant
ComponentsABO blood group (Transferase A, alpha 1-3-N-acetylgalactosaminyltransferase transferase B, alpha 1-3-galactosyltransferase)
KeywordsTRANSFERASE / Blood group transferases / glycosyltransferases / enzymes
Function / homology
Function and homology information


glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity / ABO blood group biosynthesis / fucosylgalactoside 3-alpha-galactosyltransferase activity / fucosylgalactoside 3-alpha-galactosyltransferase / glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase / hexosyltransferase activity / lipid glycosylation / Golgi cisterna membrane / protein glycosylation / antigen binding ...glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity / ABO blood group biosynthesis / fucosylgalactoside 3-alpha-galactosyltransferase activity / fucosylgalactoside 3-alpha-galactosyltransferase / glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase / hexosyltransferase activity / lipid glycosylation / Golgi cisterna membrane / protein glycosylation / antigen binding / manganese ion binding / vesicle / carbohydrate metabolic process / Golgi membrane / nucleotide binding / Golgi apparatus / extracellular region / membrane => GO:0016020
Similarity search - Function
Glycosyltransferase family 6 / Glycosyl transferase, family 6 / Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A / Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A / Nucleotide-diphospho-sugar transferases / Alpha-Beta Complex / Alpha Beta
Similarity search - Domain/homology
ABO blood group (Transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase) / Histo-blood group ABO system transferase
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.45 Å
AuthorsGagnon, S.M.L. / Legg, M.S.G. / Evans, S.V.
Funding support Canada, 1items
OrganizationGrant numberCountry
Canadian Institutes of Health Research (CIHR)MOP-77655 Canada
CitationJournal: Glycobiology / Year: 2018
Title: Conserved residues Arg188 and Asp302 are critical for active site organization and catalysis in human ABO(H) blood group A and B glycosyltransferases.
Authors: Gagnon, S.M.L. / Legg, M.S.G. / Polakowski, R. / Letts, J.A. / Persson, M. / Lin, S. / Zheng, R.B. / Rempel, B. / Schuman, B. / Haji-Ghassemi, O. / Borisova, S.N. / Palcic, M.M. / Evans, S.V.
History
DepositionNov 6, 2017Deposition site: RCSB / Processing site: RCSB
Revision 1.0Sep 19, 2018Provider: repository / Type: Initial release
Revision 1.1Jan 8, 2020Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: ABO blood group (Transferase A, alpha 1-3-N-acetylgalactosaminyltransferase transferase B, alpha 1-3-galactosyltransferase)


Theoretical massNumber of molelcules
Total (without water)34,2421
Polymers34,2421
Non-polymers00
Water4,792266
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area0 Å2
ΔGint0 kcal/mol
Surface area13760 Å2
2
A: ABO blood group (Transferase A, alpha 1-3-N-acetylgalactosaminyltransferase transferase B, alpha 1-3-galactosyltransferase)

A: ABO blood group (Transferase A, alpha 1-3-N-acetylgalactosaminyltransferase transferase B, alpha 1-3-galactosyltransferase)


Theoretical massNumber of molelcules
Total (without water)68,4832
Polymers68,4832
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation3_655-x+1,y,-z+1/21
Buried area4730 Å2
ΔGint-25 kcal/mol
Surface area22790 Å2
MethodPISA
Unit cell
Length a, b, c (Å)52.690, 150.320, 78.980
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number20
Space group name H-MC2221

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Components

#1: Protein ABO blood group (Transferase A, alpha 1-3-N-acetylgalactosaminyltransferase transferase B, alpha 1-3-galactosyltransferase) / ABO blood group (Transferase A / alpha 1-3-N-acetylgalactosaminyltransferase / transferase B / ...ABO blood group (Transferase A / alpha 1-3-N-acetylgalactosaminyltransferase / transferase B / alpha 1-3-galactosyltransferase)


Mass: 34241.547 Da / Num. of mol.: 1 / Fragment: UNP residues 54-344 / Mutation: D302A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ABO / Production host: Escherichia coli (E. coli) / References: UniProt: D3HIC2, UniProt: P16442*PLUS
#2: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 266 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.28 Å3/Da / Density % sol: 46.14 %
Crystal growTemperature: 277 K / Method: small tubes
Details: Spontaneous crystals were recovered from concentrated stocks of GTB/D302A (68 mg/mL) stored in 50 mM MOPS, pH 7.00, 0.1 M sodium chloride, 1 mM DTT, 5 mM manganese chloride, and kept at 277 K.

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Data collection

DiffractionMean temperature: 113 K
Diffraction sourceSource: SEALED TUBE / Type: RIGAKU / Wavelength: 1.5418 Å
DetectorType: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Nov 24, 2006
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.5418 Å / Relative weight: 1
ReflectionResolution: 1.45→19.89 Å / Num. obs: 55649 / % possible obs: 99.5 % / Redundancy: 4.28 % / Rmerge(I) obs: 0.029 / Rrim(I) all: 0.029 / Χ2: 0.99 / Net I/σ(I): 21.1 / Num. measured all: 239861 / Scaling rejects: 1800
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured allNum. unique obsΧ2Rejects% possible all
1.45-1.53.450.3033.61920355401.22104100
1.5-1.563.550.2384.31972755291.15104100
1.56-1.633.680.1955.12034955111.149699.9
1.63-1.723.850.1466.62144655301.04134100
1.72-1.834.130.1168.32321155720.97185100
1.83-1.974.610.09111.42578055510.91179100
1.97-2.174.750.06515.62673855990.87119100
2.17-2.484.870.0425.92738255960.8913299.9
2.48-3.124.930.02639.42784556070.8719899.2
3.12-19.894.920.01769.7281805614154996.1

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Processing

Software
NameVersionClassification
REFMAC5.8.0158refinement
d*TREK9.4SSIdata scaling
PDB_EXTRACT3.2data extraction
CrystalCleardata reduction
MOLREPphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entry 1LZ7
Resolution: 1.45→75.16 Å / Cor.coef. Fo:Fc: 0.969 / Cor.coef. Fo:Fc free: 0.964 / SU B: 0.992 / SU ML: 0.038 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.064 / ESU R Free: 0.063 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.1999 2825 5.1 %RANDOM
Rwork0.1838 ---
obs0.1846 52822 99.42 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso max: 64.58 Å2 / Biso mean: 22.236 Å2 / Biso min: 13.36 Å2
Baniso -1Baniso -2Baniso -3
1--0.59 Å20 Å20 Å2
2--0.39 Å20 Å2
3---0.19 Å2
Refinement stepCycle: final / Resolution: 1.45→75.16 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2131 0 0 266 2397
Biso mean---34.34 -
Num. residues----264
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0090.0192216
X-RAY DIFFRACTIONr_bond_other_d0.0020.022049
X-RAY DIFFRACTIONr_angle_refined_deg1.4351.9493021
X-RAY DIFFRACTIONr_angle_other_deg0.95234730
X-RAY DIFFRACTIONr_dihedral_angle_1_deg6.1935264
X-RAY DIFFRACTIONr_dihedral_angle_2_deg32.82222.885104
X-RAY DIFFRACTIONr_dihedral_angle_3_deg10.99815357
X-RAY DIFFRACTIONr_dihedral_angle_4_deg14.7371516
X-RAY DIFFRACTIONr_chiral_restr0.0840.2337
X-RAY DIFFRACTIONr_gen_planes_refined0.0070.0212440
X-RAY DIFFRACTIONr_gen_planes_other0.0010.02488
LS refinement shellResolution: 1.45→1.488 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.371 206 -
Rwork0.359 3881 -
all-4087 -
obs--99.93 %

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