The Tee13 major pilin protein is polymerised end to end into long chains at the cell surface with the aid of a pilin specific sortase. The crystal packing does not resemble this arrangement.
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Components
#1: Protein
MajorpilinbackboneproteinT-antigen, T13
Mass: 31042.721 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Isolate from a throat swab from a rheumatic fever patient Source: (gene. exp.) Streptococcus pyogenes (bacteria) / Gene: tee / Plasmid: pProExHta / Production host: Escherichia coli (E. coli) / Strain (production host): K11 / References: UniProt: A0A096ZHE3, UniProt: A2T8Y3*PLUS
Resolution: 1.9→85.74 Å / Cor.coef. Fo:Fc: 0.95 / Cor.coef. Fo:Fc free: 0.93 / SU B: 3.936 / SU ML: 0.109 / Cross valid method: THROUGHOUT / ESU R: 0.134 / ESU R Free: 0.128 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.23577
3052
5 %
RANDOM
Rwork
0.2036
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obs
0.20519
58361
99.99 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK