E3ubiquitin-proteinligaseUHRF1 / RING-type E3 ubiquitin transferase UHRF1 / Ubiquitin-like PHD and RING finger domain-containing ...RING-type E3 ubiquitin transferase UHRF1 / Ubiquitin-like PHD and RING finger domain-containing protein 1 / Ubiquitin-like-containing PHD and RING finger domains protein 1
Mass: 17778.994 Da / Num. of mol.: 3 / Fragment: unp residues 129-280 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Danio rerio (zebrafish) / Gene: uhrf1 / Production host: Escherichia coli K-12 (bacteria) References: UniProt: E7EZF3, RING-type E3 ubiquitin transferase
#2: Protein/peptide
E3ubiquitin-proteinligaseUHRF1 / Inverted CCAAT box-binding protein of 90 kDa / Nuclear protein 95 / Nuclear zinc finger protein ...Inverted CCAAT box-binding protein of 90 kDa / Nuclear protein 95 / Nuclear zinc finger protein Np95 / hNp95 / RING finger protein 106 / RING-type E3 ubiquitin transferase UHRF1 / Transcription factor ICBP90 / Ubiquitin-like PHD and RING finger domain-containing protein 1 / hUHRF1 / Ubiquitin-like-containing PHD and RING finger domains protein 1
Mass: 4340.991 Da / Num. of mol.: 1 / Fragment: unp residues 638-678 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: UHRF1, ICBP90, NP95, RNF106 / Production host: Escherichia coli K-12 (bacteria) References: UniProt: Q96T88, RING-type E3 ubiquitin transferase
Mass: 18.015 Da / Num. of mol.: 535 / Source method: isolated from a natural source / Formula: H2O
Sequence details
The sample sequence of the proteinused is different from sequence (E7EZF3). The accession of the ...The sample sequence of the proteinused is different from sequence (E7EZF3). The accession of the sequence of our sample in NCBI is NP_998242 which is also from Danio rerio (zebrafish)
-
Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
-
Sample preparation
Crystal
Density Matthews: 2.71 Å3/Da / Density % sol: 54.66 %
Crystal grow
Temperature: 277 K / Method: vapor diffusion, hanging drop Details: 0.1M Succinic acid pH7.0, 15% (v/v) Polyethylene glycol 3350
-
Data collection
Diffraction
Mean temperature: 100 K
Diffraction source
Source: SYNCHROTRON / Site: ALS / Beamline: 5.0.3 / Wavelength: 0.97741 Å