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- PDB-6aq3: Crystal structure of a trafficking protein particle complex subun... -

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Basic information

Entry
Database: PDB / ID: 6aq3
TitleCrystal structure of a trafficking protein particle complex subunit 3 from Naegleria fowleri covalently bound to palmitic acid
Componentstrafficking protein particle complex subunit 3
KeywordsTRANSPORT PROTEIN / structural genomics / brain eating bacteria / NIAID / lipid / Seattle Structural Genomics Center for Infectious Disease / SSGCID
Function / homology
Function and homology information


TRAPP complex / cis-Golgi network membrane / intra-Golgi vesicle-mediated transport / endoplasmic reticulum to Golgi vesicle-mediated transport / endoplasmic reticulum / cytosol
Similarity search - Function
Trafficking protein particle complex subunit 3 / Bet3 family / Transport protein particle (TRAPP) component / Transport protein particle (TRAPP) component / Muramoyl-pentapeptide Carboxypeptidase; domain 2 / NO signalling/Golgi transport ligand-binding domain superfamily / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
PALMITIC ACID / Trafficking protein particle complex subunit
Similarity search - Component
Biological speciesNaegleria fowleri (brain-eating amoeba)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å
AuthorsEdwards, T.E. / Seattle Structural Genomics Center for Infectious Disease (SSGCID)
CitationJournal: To Be Published
Title: Crystal structure of a trafficking protein particle complex subunit 3 from Naegleria fowleri covalently bound to palmitic acid
Authors: Edwards, T.E. / Abendroth, J. / Lorimer, D.D.
History
DepositionAug 18, 2017Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 30, 2017Provider: repository / Type: Initial release
Revision 1.1Oct 4, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id
Revision 1.2Oct 30, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: trafficking protein particle complex subunit 3
B: trafficking protein particle complex subunit 3
C: trafficking protein particle complex subunit 3
D: trafficking protein particle complex subunit 3
E: trafficking protein particle complex subunit 3
F: trafficking protein particle complex subunit 3
hetero molecules


Theoretical massNumber of molelcules
Total (without water)136,78923
Polymers134,5676
Non-polymers2,22117
Water1,982110
1
A: trafficking protein particle complex subunit 3
C: trafficking protein particle complex subunit 3
hetero molecules


Theoretical massNumber of molelcules
Total (without water)45,5557
Polymers44,8562
Non-polymers6995
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4570 Å2
ΔGint-18 kcal/mol
Surface area14530 Å2
MethodPISA
2
B: trafficking protein particle complex subunit 3
D: trafficking protein particle complex subunit 3
hetero molecules


Theoretical massNumber of molelcules
Total (without water)45,6178
Polymers44,8562
Non-polymers7616
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5050 Å2
ΔGint-19 kcal/mol
Surface area15100 Å2
MethodPISA
3
E: trafficking protein particle complex subunit 3
F: trafficking protein particle complex subunit 3
hetero molecules


Theoretical massNumber of molelcules
Total (without water)45,6178
Polymers44,8562
Non-polymers7616
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5120 Å2
ΔGint-14 kcal/mol
Surface area15290 Å2
MethodPISA
Unit cell
Length a, b, c (Å)53.640, 78.670, 80.570
Angle α, β, γ (deg.)114.550, 87.670, 95.020
Int Tables number1
Space group name H-MP1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and ((resid 16 through 19 and (name N...
21(chain B and ((resid 16 through 19 and (name N...
31(chain C and (resid 16 through 35 or (resid 36...
41(chain D and ((resid 16 through 19 and (name N...
51(chain E and ((resid 16 through 19 and (name N...
61(chain F and ((resid 16 through 19 and (name N...

NCS domain segments:

Ens-ID: 1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11GLUGLUASNASN(chain A and ((resid 16 through 19 and (name N...AA16 - 1924 - 27
12GLUGLUPLMPLM(chain A and ((resid 16 through 19 and (name N...AA - G16 - 20124
13GLUGLUPLMPLM(chain A and ((resid 16 through 19 and (name N...AA - G16 - 20124
14GLUGLUPLMPLM(chain A and ((resid 16 through 19 and (name N...AA - G16 - 20124
15GLUGLUPLMPLM(chain A and ((resid 16 through 19 and (name N...AA - G16 - 20124
21GLUGLUASNASN(chain B and ((resid 16 through 19 and (name N...BB16 - 1924 - 27
22THRTHRPLMPLM(chain B and ((resid 16 through 19 and (name N...BB - J11 - 20119
23THRTHRPLMPLM(chain B and ((resid 16 through 19 and (name N...BB - J11 - 20119
24THRTHRPLMPLM(chain B and ((resid 16 through 19 and (name N...BB - J11 - 20119
25THRTHRPLMPLM(chain B and ((resid 16 through 19 and (name N...BB - J11 - 20119
31GLUGLULEULEU(chain C and (resid 16 through 35 or (resid 36...CC16 - 3524 - 43
32LYSLYSASPASP(chain C and (resid 16 through 35 or (resid 36...CC36 - 3744 - 45
33ILEILEPLMPLM(chain C and (resid 16 through 35 or (resid 36...CC - N15 - 20123
34ILEILEPLMPLM(chain C and (resid 16 through 35 or (resid 36...CC - N15 - 20123
35ILEILEPLMPLM(chain C and (resid 16 through 35 or (resid 36...CC - N15 - 20123
36ILEILEPLMPLM(chain C and (resid 16 through 35 or (resid 36...CC - N15 - 20123
41GLUGLUASNASN(chain D and ((resid 16 through 19 and (name N...DD16 - 1924 - 27
42PHEPHEPLMPLM(chain D and ((resid 16 through 19 and (name N...DD - P12 - 20120
43PHEPHEPLMPLM(chain D and ((resid 16 through 19 and (name N...DD - P12 - 20120
44PHEPHEPLMPLM(chain D and ((resid 16 through 19 and (name N...DD - P12 - 20120
45PHEPHEPLMPLM(chain D and ((resid 16 through 19 and (name N...DD - P12 - 20120
51GLUGLUASNASN(chain E and ((resid 16 through 19 and (name N...EE16 - 1924 - 27
52LYSLYSEDOEDO(chain E and ((resid 16 through 19 and (name N...EE - T14 - 20322
53LYSLYSEDOEDO(chain E and ((resid 16 through 19 and (name N...EE - T14 - 20322
54LYSLYSEDOEDO(chain E and ((resid 16 through 19 and (name N...EE - T14 - 20322
55LYSLYSEDOEDO(chain E and ((resid 16 through 19 and (name N...EE - T14 - 20322
61GLUGLUASNASN(chain F and ((resid 16 through 19 and (name N...FF16 - 1924 - 27
62ASNASNEDOEDO(chain F and ((resid 16 through 19 and (name N...FF - U13 - 20121
63ASNASNEDOEDO(chain F and ((resid 16 through 19 and (name N...FF - U13 - 20121
64ASNASNEDOEDO(chain F and ((resid 16 through 19 and (name N...FF - U13 - 20121
65ASNASNEDOEDO(chain F and ((resid 16 through 19 and (name N...FF - U13 - 20121

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Components

#1: Protein
trafficking protein particle complex subunit 3


Mass: 22427.902 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Naegleria fowleri (brain-eating amoeba)
Strain: ATCC 30863 / Gene: NF0066220 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A2D0TCJ8*PLUS
#2: Chemical
ChemComp-PLM / PALMITIC ACID


Mass: 256.424 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C16H32O2
#3: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 11 / Source method: obtained synthetically / Formula: C2H6O2
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 110 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.29 Å3/Da / Density % sol: 46.27 %
Crystal growTemperature: 289 K / Method: vapor diffusion, sitting drop / pH: 5.5
Details: 22 mg/mL NafoA.00964.a.B1 PS38152 against JCSG+ screen condition H10 (0.2 M ammonium sulfate, 0.1 M Bis-Tris, pH 5.5, 25% PEG3350), cryoprotectant: 15% ethylene glycol, crystal tracking ID ...Details: 22 mg/mL NafoA.00964.a.B1 PS38152 against JCSG+ screen condition H10 (0.2 M ammonium sulfate, 0.1 M Bis-Tris, pH 5.5, 25% PEG3350), cryoprotectant: 15% ethylene glycol, crystal tracking ID 288103h10, unique puck ID afj8-5

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 21-ID-G / Wavelength: 0.97856 Å
DetectorType: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Aug 7, 2017
RadiationMonochromator: diamond(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97856 Å / Relative weight: 1
ReflectionResolution: 2.4→33.381 Å / Num. obs: 45852 / % possible obs: 98.1 % / Observed criterion σ(I): -3 / Redundancy: 3.868 % / Biso Wilson estimate: 53.96 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.043 / Rrim(I) all: 0.05 / Χ2: 1.023 / Net I/σ(I): 18.62
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. unique obsCC1/2Rrim(I) all% possible all
2.4-2.463.9750.552.7533730.8280.63697.6
2.46-2.533.9510.4433.3832950.8950.51397.6
2.53-2.63.9360.3554.332050.920.41198
2.6-2.683.780.2675.6431120.960.31296.8
2.68-2.773.9310.236.2529920.9640.26798
2.77-2.873.9790.1598.2929380.9840.18498.3
2.87-2.983.9510.1211.1428410.9890.13898.1
2.98-3.13.9580.09213.7227040.9930.10798.4
3.1-3.243.9460.06817.7925980.9960.07998.4
3.24-3.393.930.05521.9125250.9970.06498.4
3.39-3.583.7390.04626.5323830.9970.05498.2
3.58-3.793.7060.03730.3922350.9980.04497.6
3.79-4.063.6430.03333.5620590.9980.03997.5
4.06-4.383.8030.02938.220050.9980.03498.9
4.38-4.83.7840.02740.4418230.9990.03299.2
4.8-5.373.7940.02740.4516470.9990.03198.7
5.37-6.23.820.02739.6714590.9990.03299.2
6.2-7.593.8130.02541.1912190.9990.02998.8
7.59-10.733.7620.02244.129500.9990.02598.7
10.73-33.3813.5660.02343.644890.9980.02794

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Processing

Software
NameVersionClassification
XSCALEdata scaling
PHENIXrefinement
PDB_EXTRACT3.22data extraction
XDSdata reduction
MOLREPphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entry 1SZ7

1sz7


Resolution: 2.4→33.381 Å / SU ML: 0.31 / Cross valid method: THROUGHOUT / σ(F): 1.99 / Phase error: 25.68
RfactorNum. reflection% reflection
Rfree0.2086 2024 4.42 %
Rwork0.1712 --
obs0.173 45811 98.22 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 146.37 Å2 / Biso mean: 67.4393 Å2 / Biso min: 28.66 Å2
Refinement stepCycle: final / Resolution: 2.4→33.381 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7715 0 146 111 7972
Biso mean--64.73 59.01 -
Num. residues----1014
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0087990
X-RAY DIFFRACTIONf_angle_d0.9210742
X-RAY DIFFRACTIONf_chiral_restr0.0551228
X-RAY DIFFRACTIONf_plane_restr0.0061365
X-RAY DIFFRACTIONf_dihedral_angle_d15.4024887
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A4363X-RAY DIFFRACTION8.697TORSIONAL
12B4363X-RAY DIFFRACTION8.697TORSIONAL
13C4363X-RAY DIFFRACTION8.697TORSIONAL
14D4363X-RAY DIFFRACTION8.697TORSIONAL
15E4363X-RAY DIFFRACTION8.697TORSIONAL
16F4363X-RAY DIFFRACTION8.697TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 14

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.4001-2.46010.30741520.25483090324297
2.4601-2.52660.30491360.23273138327498
2.5266-2.60090.24381460.2273110325698
2.6009-2.68480.32081340.24163114324897
2.6848-2.78070.25491260.21933099322598
2.7807-2.8920.24611340.22013137327198
2.892-3.02350.29081310.20883149328098
3.0235-3.18280.2611580.20373154331299
3.1828-3.3820.25431380.1953134327299
3.382-3.64290.24011460.17993146329298
3.6429-4.00890.19231540.16583061321597
4.0089-4.58770.16161820.12773131331399
4.5877-5.77520.16991300.13853193332399
5.7752-33.38430.17891570.15373131328899
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
18.258-2.41311.14944.6757-1.80239.06920.25850.2552-0.0373-0.7567-0.0860.1310.58760.9075-0.21980.525-0.0885-0.00240.46-0.06420.31868.889-3.2841-13.5685
28.47760.8746-1.17053.0881-2.9057.85240.13510.2038-0.84640.0648-0.1013-0.01140.6531-0.2117-0.10520.5286-0.031-0.0180.2615-0.06250.3947-4.3952-2.9174-7.4968
32.13461.2291.9841.03210.85774.9175-0.0833-0.906-1.05720.34030.21920.54010.7791-0.47950.07860.5789-0.10890.04350.53510.26890.6306-6.319-6.38316.8135
41.27880.1584-0.72442.21051.89362.2646-0.0392-0.5095-0.12050.62340.0919-0.01330.75170.86620.11880.7032-0.0581-0.05170.64020.1050.37924.6615-3.24529.0239
57.9245-4.1915-4.92455.18164.50816.7771-0.0729-1.00320.21930.39350.268-0.13580.04860.4422-0.28930.536-0.10820.01960.4721-0.0080.33711.04183.2497.2394
62.8367-3.6761-1.97665.82653.71218.8569-0.2476-0.74160.72540.12870.2591-0.9331-0.47691.42160.22320.5484-0.19430.04440.5565-0.02390.649613.49256.1453-7.6594
77.7907-1.4233-2.21042.7311-1.13922.51880.1653-0.42510.28050.1741-0.11760.051-0.05470.24290.03210.4794-0.0520.01470.33080.03530.3409-1.19243.4001-0.8487
83.4399-2.8055-2.12174.4216-0.31264.0331-0.04810.040.87250.0784-0.02410.287-0.26370.10620.05710.4314-0.1070.01710.35260.00760.3379-9.02915.56543.0464
97.8828-1.3814-0.07067.5321-2.5248.3506-0.0737-0.03770.33250.32860.4096-0.5731-0.37430.3961-0.13260.3457-0.0493-0.10280.5064-0.04110.51124.2669-22.365129.7729
107.12852.11730.09947.7872.86593.55680.05820.4084-0.23750.071-0.08250.48550.0866-0.43890.02790.30050.02830.01810.44670.05530.325613.2682-34.184327.8164
114.10750.8725-0.24819.0048-4.45084.1255-0.0320.6633-0.598-0.1517-0.2695-0.32330.29260.33890.33580.33450.01190.05340.6988-0.14450.468221.9019-43.162622.1254
125.09161.45640.07185.4055-1.0562.7659-0.07860.0497-0.25010.58-0.0787-0.9998-0.10910.69060.10890.36390.0268-0.08570.6233-0.10560.447824.2205-37.375832.2167
136.98631.7635-2.31862.51131.20013.00610.0963-0.04610.14260.4043-0.13890.0202-0.295-0.0333-0.0040.41540.07620.0220.2364-0.01840.38765.433-7.7164-21.4801
144.9084-4.29343.82944.138-3.68833.418-0.2598-1.9377-0.51582.63641.0539-0.17370.2516-0.4602-0.37650.86660.1734-0.12780.89660.16180.779119.0506-20.0805-10.3018
156.4588-1.7330.58754.4839-0.38452.30570.1638-0.006-1.10880.1271-0.1322-0.20650.47460.4675-0.07530.62120.0849-0.03450.35990.05310.672412.4169-27.8357-21.337
168.3805-2.24031.18533.7469-0.31812.21960.24460.4471-0.3225-0.2046-0.2621-0.38280.25960.25610.07970.4230.09770.02270.32540.03860.399.0466-17.9934-27.3288
176.2367-0.08681.48333.9002-0.06454.1917-0.2589-0.3905-0.2190.32110.59080.228-0.46570.0929-0.23920.44070.01020.03570.62060.16190.480415.7609-18.594229.5575
184.15990.68111.36129.28090.16116.46280.1270.09470.02630.1159-0.0183-1.2185-1.02690.96440.18960.4621-0.039-0.1480.5660.06460.508625.8406-9.599626.7129
191.60960.8337-1.60780.5067-0.87871.735-1.22370.90440.2061-0.22981.2786-0.09750.6039-0.83040.27111.3655-0.3822-0.09341.05920.08061.022130.1959-4.06588.1145
205.0235-0.38730.63363.6465-0.12152.9254-0.15880.09520.27650.22290.05680.1582-0.5657-0.18880.10170.5364-0.01540.0310.52750.10180.311416.7662-3.501121.8506
215.0250.0052-1.23377.21412.21898.7853-0.3519-0.0752-0.213-0.0027-0.10650.34150.45090.27830.41790.31260.06680.02020.3829-0.00240.6811-5.2125-51.4272-6.1047
229.2328-4.8903-0.41732.7401-0.06364.9230.0445-0.2305-0.4719-0.6375-0.00421.0982-0.1231-0.7928-0.09110.5366-0.0289-0.06290.5122-0.09920.6578-15.5828-47.1136-15.0469
231.7414-0.39022.04450.3975-0.44872.4627-0.5184-0.38161.1935-0.46830.4026-0.21280.1352-0.4054-0.03651.06720.1517-0.27090.7887-0.15841.2346-20.1733-28.4305-19.649
242.67781.4389-1.01721.42070.4765.6144-0.16190.0931-0.1261-0.1858-0.135-0.1519-0.01940.20610.24650.52120.0883-0.0240.35710.01020.7628-3.2504-41.0975-18.6486
256.42242.46333.68941.29170.47015.2370.28610.5043-0.0451-0.8007-0.32540.26810.2261-0.2250.10740.71380.1814-0.12140.4092-0.12480.8703-14.5989-44.0532-22.2054
262.2964-0.6127-2.07611.2068-0.51982.50770.08851.3079-0.3862-0.5752-0.2255-0.1825-0.07060.4583-0.05240.66330.1057-0.03120.3245-0.09430.878-8.6383-42.6151-24.9016
270.4230.1213-0.39811.46120.82333.9115-0.0761-0.46520.45740.11010.1469-0.0953-0.10640.3631-0.08550.32470.0431-0.02730.6125-0.16430.6407-8.1944-49.37496.0297
283.4423-1.19540.39754.0742-1.49429.6488-0.2713-1.0620.21530.38990.37580.1851-0.1659-0.1515-0.10120.37780.111-0.02641.0322-0.29690.7718-14.7836-45.734615.7891
291.7050.75822.62264.0894-1.43558.8129-0.6871-1.15350.7355-0.46120.53551.77360.0048-1.74990.12640.5209-0.0173-0.02071.0065-0.1581.0059-23.4841-57.73822.418
300.77640.02422.09083.4238-0.10713.5481-0.0757-1.1294-0.03380.19420.15620.24470.373-0.45680.05560.44150.0646-0.03371.0167-0.26140.5842-9.5366-53.988315.6156
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 16 through 40 )A16 - 40
2X-RAY DIFFRACTION2chain 'A' and (resid 41 through 67 )A41 - 67
3X-RAY DIFFRACTION3chain 'A' and (resid 68 through 93 )A68 - 93
4X-RAY DIFFRACTION4chain 'A' and (resid 94 through 100 )A94 - 100
5X-RAY DIFFRACTION5chain 'A' and (resid 101 through 119 )A101 - 119
6X-RAY DIFFRACTION6chain 'A' and (resid 120 through 136 )A120 - 136
7X-RAY DIFFRACTION7chain 'A' and (resid 137 through 168 )A137 - 168
8X-RAY DIFFRACTION8chain 'A' and (resid 169 through 181 )A169 - 181
9X-RAY DIFFRACTION9chain 'B' and (resid 11 through 40 )B11 - 40
10X-RAY DIFFRACTION10chain 'B' and (resid 41 through 76 )B41 - 76
11X-RAY DIFFRACTION11chain 'B' and (resid 77 through 107 )B77 - 107
12X-RAY DIFFRACTION12chain 'B' and (resid 108 through 181 )B108 - 181
13X-RAY DIFFRACTION13chain 'C' and (resid 15 through 57 )C15 - 57
14X-RAY DIFFRACTION14chain 'C' and (resid 58 through 67 )C58 - 67
15X-RAY DIFFRACTION15chain 'C' and (resid 68 through 119 )C68 - 119
16X-RAY DIFFRACTION16chain 'C' and (resid 120 through 181 )C120 - 181
17X-RAY DIFFRACTION17chain 'D' and (resid 12 through 40 )D12 - 40
18X-RAY DIFFRACTION18chain 'D' and (resid 41 through 66 )D41 - 66
19X-RAY DIFFRACTION19chain 'D' and (resid 67 through 76 )D67 - 76
20X-RAY DIFFRACTION20chain 'D' and (resid 77 through 181 )D77 - 181
21X-RAY DIFFRACTION21chain 'E' and (resid 14 through 40 )E14 - 40
22X-RAY DIFFRACTION22chain 'E' and (resid 41 through 66 )E41 - 66
23X-RAY DIFFRACTION23chain 'E' and (resid 67 through 76 )E67 - 76
24X-RAY DIFFRACTION24chain 'E' and (resid 77 through 136 )E77 - 136
25X-RAY DIFFRACTION25chain 'E' and (resid 137 through 158 )E137 - 158
26X-RAY DIFFRACTION26chain 'E' and (resid 159 through 184 )E159 - 184
27X-RAY DIFFRACTION27chain 'F' and (resid 13 through 86 )F13 - 86
28X-RAY DIFFRACTION28chain 'F' and (resid 87 through 119 )F87 - 119
29X-RAY DIFFRACTION29chain 'F' and (resid 120 through 136 )F120 - 136
30X-RAY DIFFRACTION30chain 'F' and (resid 137 through 181 )F137 - 181

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