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- PDB-4cfo: Structure of Lytic Transglycosylase MltC from Escherichia coli in... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4cfo | |||||||||
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Title | Structure of Lytic Transglycosylase MltC from Escherichia coli in complex with tetrasaccharide at 2.9 A resolution. | |||||||||
![]() | MLTC | |||||||||
![]() | HYDROLASE | |||||||||
Function / homology | ![]() lytic endotransglycosylase activity / : / lytic transglycosylase activity / peptidoglycan metabolic process / hydrolase activity, acting on glycosyl bonds / peptidoglycan catabolic process / cell outer membrane / cell wall organization / cell wall macromolecule catabolic process / cellular response to oxidative stress / outer membrane-bounded periplasmic space Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Artola-Recolons, C. / Bernardo-Garcia, N. / Mobashery, S. / Hermoso, J.A. | |||||||||
![]() | ![]() Title: Structure and Cell Wall Cleavage by Modular Lytic Transglycosylase Mltc of Escherichia Coli. Authors: Artola-Recolons, C. / Lee, M. / Bernardo-Garcia, N. / Blazquez, B. / Hesek, D. / Bartual, S.G. / Mahasenan, K.V. / Lastochkin, E. / Pi, H. / Boggess, B. / Meindl, K. / Uson, I. / Fisher, J.F. ...Authors: Artola-Recolons, C. / Lee, M. / Bernardo-Garcia, N. / Blazquez, B. / Hesek, D. / Bartual, S.G. / Mahasenan, K.V. / Lastochkin, E. / Pi, H. / Boggess, B. / Meindl, K. / Uson, I. / Fisher, J.F. / Mobashery, S. / Hermoso, J.A. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 271.4 KB | Display | ![]() |
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PDB format | ![]() | 220.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 29.2 KB | Display | |
Data in CIF | ![]() | 39.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4c5fSC ![]() 4cfpC ![]() 4chxC C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 38259.070 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Polysaccharide | Type: oligosaccharide / Mass: 986.968 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 45.13 % / Description: NONE |
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Crystal grow | Details: 18% PEG 3350, 0.2M TRI-AMMONIUM CITRATE PH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 18, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97948 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→45.03 Å / Num. obs: 18455 / % possible obs: 99.8 % / Observed criterion σ(I): 2 / Redundancy: 4.2 % / Rmerge(I) obs: 0.14 / Net I/σ(I): 3.6 |
Reflection shell | Resolution: 2.7→2.83 Å / Redundancy: 3.9 % / Rmerge(I) obs: 0.47 / Mean I/σ(I) obs: 1.8 / % possible all: 99.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4C5F Resolution: 2.9→14.93 Å / Cor.coef. Fo:Fc: 0.929 / Cor.coef. Fo:Fc free: 0.837 / SU B: 47.622 / SU ML: 0.399 / Cross valid method: THROUGHOUT / ESU R Free: 0.492 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.26 Å2
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Refinement step | Cycle: LAST / Resolution: 2.9→14.93 Å
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Refine LS restraints |
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