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- PDB-6aop: Crystal structure of the A/Brisbane/10/2007 (H3N2) influenza viru... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6aop | ||||||||||||
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Title | Crystal structure of the A/Brisbane/10/2007 (H3N2) influenza virus hemagglutinin L194P mutant apo form | ||||||||||||
![]() | (Hemagglutinin ...) x 2 | ||||||||||||
![]() | VIRAL PROTEIN / Influenza A virus / hemagglutinin / mutant / receptor binding / antigenicity | ||||||||||||
Function / homology | ![]() viral budding from plasma membrane / clathrin-dependent endocytosis of virus by host cell / host cell surface receptor binding / apical plasma membrane / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Wu, N.C. / Wilson, I.A. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: A structural explanation for the low effectiveness of the seasonal influenza H3N2 vaccine. Authors: Wu, N.C. / Zost, S.J. / Thompson, A.J. / Oyen, D. / Nycholat, C.M. / McBride, R. / Paulson, J.C. / Hensley, S.E. / Wilson, I.A. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 219.8 KB | Display | ![]() |
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PDB format | ![]() | 176 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.3 MB | Display | ![]() |
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Full document | ![]() | 1.3 MB | Display | |
Data in XML | ![]() | 21.5 KB | Display | |
Data in CIF | ![]() | 30.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6aoqC ![]() 6aorC ![]() 6aosC ![]() 6aotC ![]() 6aouC ![]() 6aovC ![]() 4o5nS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Hemagglutinin ... , 2 types, 2 molecules AB
#1: Protein | Mass: 35907.422 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: A/Brisbane/10/2007(H3N2) / Gene: HA / Production host: ![]() |
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#2: Protein | Mass: 19953.174 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: A/Brisbane/10/2007(H3N2) / Gene: HA / Production host: ![]() |
-Sugars , 3 types, 7 molecules ![](data/chem/img/NAG.gif)
#3: Polysaccharide | Source method: isolated from a genetically manipulated source #4: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #5: Sugar | ChemComp-NAG / |
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-Non-polymers , 1 types, 164 molecules ![](data/chem/img/HOH.gif)
#6: Water | ChemComp-HOH / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.33 Å3/Da / Density % sol: 63.11 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 10.5 / Details: 0.1 M CAPS pH 10.5 and 29% PEG 400 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Nov 29, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0332 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→50 Å / Num. obs: 34068 / % possible obs: 99.6 % / Redundancy: 16.2 % / Biso Wilson estimate: 40 Å2 / CC1/2: 1 / Rpim(I) all: 0.03 / Rsym value: 0.1 / Net I/σ(I): 31.7 |
Reflection shell | Resolution: 2.3→2.37 Å / Redundancy: 9 % / Mean I/σ(I) obs: 1.6 / Num. unique obs: 2928 / CC1/2: 0.79 / Rpim(I) all: 0.22 / Rsym value: 0.7 / % possible all: 95.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4O5N Resolution: 2.3→50 Å / Cor.coef. Fo:Fc: 0.949 / Cor.coef. Fo:Fc free: 0.928 / SU B: 14.575 / SU ML: 0.172 / Cross valid method: THROUGHOUT / ESU R: 0.244 / ESU R Free: 0.197 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 60.36 Å2
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Refinement step | Cycle: 1 / Resolution: 2.3→50 Å
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Refine LS restraints |
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