Hydrolases; Acting on ester bonds; Exonucleases that are active with either ribo- or deoxyribonucleic acids and produce 5'-phosphomonoesters / 3'-5'-RNA exonuclease activity / nucleic acid binding / cytoplasm Similarity search - Function
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.9796 Å / Relative weight: 1
Reflection
Resolution: 2.21→50 Å / Num. obs: 21675 / % possible obs: 86.2 % / Redundancy: 4.8 % / Net I/σ(I): 43.9
Reflection shell
Resolution: 2.21→2.25 Å
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Processing
Software
Name
Version
Classification
REFMAC
5.8.0189
refinement
MxDC
datacollection
HKL-2000
datascaling
Refinement
Resolution: 2.21→47.02 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.91 / SU B: 6.515 / SU ML: 0.163 / Cross valid method: THROUGHOUT / ESU R: 0.29 / ESU R Free: 0.25 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.27018
1031
4.8 %
RANDOM
Rwork
0.19304
-
-
-
obs
0.19674
20644
86.35 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å