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- PDB-5ze9: Crystal structure of AMP-PNP bound mutant A3B3 complex from Enter... -

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Basic information

Entry
Database: PDB / ID: 5ze9
TitleCrystal structure of AMP-PNP bound mutant A3B3 complex from Enterococcus hirae V-ATPase
Components(V-type sodium ATPase ...) x 2
KeywordsHYDROLASE / P-loop
Function / homology
Function and homology information


Na+-transporting two-sector ATPase / sodium-transporting ATP synthase activity, rotational mechanism / sodium-transporting ATPase activity, rotational mechanism / proton-transporting ATP synthase complex / vacuolar acidification / proton motive force-driven plasma membrane ATP synthesis / sodium ion transport / phagocytic vesicle / proton-transporting ATPase activity, rotational mechanism / proton-transporting ATP synthase activity, rotational mechanism ...Na+-transporting two-sector ATPase / sodium-transporting ATP synthase activity, rotational mechanism / sodium-transporting ATPase activity, rotational mechanism / proton-transporting ATP synthase complex / vacuolar acidification / proton motive force-driven plasma membrane ATP synthesis / sodium ion transport / phagocytic vesicle / proton-transporting ATPase activity, rotational mechanism / proton-transporting ATP synthase activity, rotational mechanism / lysosomal membrane / ATP binding
Similarity search - Function
Rossmann fold - #12240 / Bovine Mitochondrial F1-ATPase, ATP Synthase Beta Chain; Chain D, domain3 / Bovine Mitochondrial F1-atpase; Atp Synthase Beta Chain; Chain D, domain 3 / V-type ATP synthase regulatory subunit B/beta / V-type ATP synthase catalytic alpha chain / ATPsynthase alpha/beta subunit, N-terminal extension / ATPsynthase alpha/beta subunit N-term extension / RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain / ATPase, F1/V1 complex, beta/alpha subunit, C-terminal / ATP synthase subunit alpha, N-terminal domain-like superfamily ...Rossmann fold - #12240 / Bovine Mitochondrial F1-ATPase, ATP Synthase Beta Chain; Chain D, domain3 / Bovine Mitochondrial F1-atpase; Atp Synthase Beta Chain; Chain D, domain 3 / V-type ATP synthase regulatory subunit B/beta / V-type ATP synthase catalytic alpha chain / ATPsynthase alpha/beta subunit, N-terminal extension / ATPsynthase alpha/beta subunit N-term extension / RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain / ATPase, F1/V1 complex, beta/alpha subunit, C-terminal / ATP synthase subunit alpha, N-terminal domain-like superfamily / ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain superfamily / ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain / ATP synthase alpha/beta family, beta-barrel domain / ATPase, alpha/beta subunit, nucleotide-binding domain, active site / ATP synthase alpha and beta subunits signature. / ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain / ATP synthase alpha/beta family, nucleotide-binding domain / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / P-loop containing nucleotide triphosphate hydrolases / Beta Barrel / P-loop containing nucleoside triphosphate hydrolase / Rossmann fold / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Beta / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / V-type sodium ATPase catalytic subunit A / V-type sodium ATPase subunit B
Similarity search - Component
Biological speciesEnterococcus hirae (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.102 Å
AuthorsMaruyama, S. / Suzuki, K. / Sasaki, H. / Mizutani, K. / Saito, Y. / Imai, F.L. / Ishizuka-Katsura, Y. / Shirouzu, M. / Ichiro, Y. / Murata, T.
CitationJournal: Sci Adv / Year: 2019
Title: Metastable asymmetrical structure of a shaftless V1motor.
Authors: Maruyama, S. / Suzuki, K. / Imamura, M. / Sasaki, H. / Matsunami, H. / Mizutani, K. / Saito, Y. / Imai, F.L. / Ishizuka-Katsura, Y. / Kimura-Someya, T. / Shirouzu, M. / Uchihashi, T. / Ando, ...Authors: Maruyama, S. / Suzuki, K. / Imamura, M. / Sasaki, H. / Matsunami, H. / Mizutani, K. / Saito, Y. / Imai, F.L. / Ishizuka-Katsura, Y. / Kimura-Someya, T. / Shirouzu, M. / Uchihashi, T. / Ando, T. / Yamato, I. / Murata, T.
History
DepositionFeb 27, 2018Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Feb 6, 2019Provider: repository / Type: Initial release
Revision 1.1Feb 20, 2019Group: Data collection / Database references / Category: citation / citation_author
Item: _citation.page_first / _citation.page_last ..._citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID
Revision 1.2Nov 22, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: V-type sodium ATPase catalytic subunit A
B: V-type sodium ATPase catalytic subunit A
C: V-type sodium ATPase catalytic subunit A
D: V-type sodium ATPase subunit B
E: V-type sodium ATPase subunit B
F: V-type sodium ATPase subunit B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)359,73552
Polymers354,3566
Non-polymers5,37846
Water23,4011299
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area39260 Å2
ΔGint-99 kcal/mol
Surface area106760 Å2
MethodPISA
Unit cell
Length a, b, c (Å)105.830, 151.370, 235.490
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121

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Components

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V-type sodium ATPase ... , 2 types, 6 molecules ABCDEF

#1: Protein V-type sodium ATPase catalytic subunit A / Na(+)-translocating ATPase subunit A / V-type sodium pump catalytic subunit A


Mass: 66347.797 Da / Num. of mol.: 3 / Fragment: NtpA
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258) (bacteria)
Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258
Gene: ntpA, EHR_08260 / Production host: Escherichia coli (E. coli) / References: UniProt: Q08636, EC: 3.6.3.15
#2: Protein V-type sodium ATPase subunit B / Na(+)-translocating ATPase subunit B / V-type sodium pump subunit B


Mass: 51770.914 Da / Num. of mol.: 3 / Fragment: NtpB / Mutation: L65Y
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258) (bacteria)
Strain: ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258
Gene: ntpB, EHR_08265 / Production host: Escherichia coli (E. coli) / References: UniProt: Q08637

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Non-polymers , 5 types, 1345 molecules

#3: Chemical ChemComp-ANP / PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER


Mass: 506.196 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C10H17N6O12P3 / Comment: AMP-PNP, energy-carrying molecule analogue*YM
#4: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Mg
#5: Chemical...
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 39 / Source method: obtained synthetically / Formula: C3H8O3
#6: Chemical ChemComp-MES / 2-(N-MORPHOLINO)-ETHANESULFONIC ACID


Mass: 195.237 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H13NO4S / Comment: pH buffer*YM
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1299 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.66 Å3/Da / Density % sol: 53.78 %
Crystal growTemperature: 296 K / Method: vapor diffusion, sitting drop / pH: 6.5
Details: 28% PEG 3350, 20 mM Bis-Tris propane pH 6.5, 0.1 M NaCl, 0.1 M ammonium acetate

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Photon Factory / Beamline: BL-17A / Wavelength: 0.98 Å
DetectorType: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: Jun 21, 2016
RadiationMonochromator: Cryo-cooled channel-cut Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.98 Å / Relative weight: 1
ReflectionResolution: 2.1→60 Å / Num. obs: 218240 / % possible obs: 99.7 % / Redundancy: 6.6 % / Biso Wilson estimate: 30.64 Å2 / Net I/σ(I): 11.32
Reflection shellResolution: 2.1→2.23 Å

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Phasing

PhasingMethod: molecular replacement
Phasing MRR rigid body: 0.602
Highest resolutionLowest resolution
Rotation49.75 Å2.29 Å

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Processing

Software
NameVersionClassificationNB
XDSdata reduction
HKL-2000data scaling
MOLREP11.2.05phasing
PHENIX1.11.1_2575refinement
PDB_EXTRACT3.24data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3VR6

3vr6


Resolution: 2.102→54.869 Å / SU ML: 0.23 / Cross valid method: THROUGHOUT / σ(F): 1.36 / Phase error: 19.28
RfactorNum. reflection% reflection
Rfree0.1955 10953 5.02 %
Rwork0.1659 --
obs0.1675 218240 99.46 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 134.05 Å2 / Biso mean: 38.9367 Å2 / Biso min: 15.41 Å2
Refinement stepCycle: final / Resolution: 2.102→54.869 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms24403 0 342 1299 26044
Biso mean--54.49 40.55 -
Num. residues----3131
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00625204
X-RAY DIFFRACTIONf_angle_d0.77434063
X-RAY DIFFRACTIONf_chiral_restr0.053809
X-RAY DIFFRACTIONf_plane_restr0.0044423
X-RAY DIFFRACTIONf_dihedral_angle_d14.79415320
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 30

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.102-2.12590.31933290.28836105643489
2.1259-2.15090.30093670.254268737240100
2.1509-2.17710.28823620.243968357197100
2.1771-2.20470.27273480.236169447292100
2.2047-2.23370.29493530.233768517204100
2.2337-2.26430.29493170.26669027219100
2.2643-2.29660.26223630.219769007263100
2.2966-2.33090.25983650.208868587223100
2.3309-2.36730.24333820.19768997281100
2.3673-2.40610.23273520.198669077259100
2.4061-2.44760.23213950.194868277222100
2.4476-2.49210.23023780.185568877265100
2.4921-2.54010.22933680.184868847252100
2.5401-2.59190.20913520.178269177269100
2.5919-2.64830.2223720.180269477319100
2.6483-2.70990.21483650.175168837248100
2.7099-2.77760.22723850.174568657250100
2.7776-2.85270.2053490.166869437292100
2.8527-2.93670.21813350.171869437278100
2.9367-3.03140.21473560.169269537309100
3.0314-3.13980.20173660.169369177283100
3.1398-3.26550.19293450.163669587303100
3.2655-3.41410.17273890.157169647353100
3.4141-3.5940.16693830.145869047287100
3.594-3.81920.16543540.136769867340100
3.8192-4.1140.16263820.137569937375100
4.114-4.52780.13443630.11970227385100
4.5278-5.18250.15663790.128170177396100
5.1825-6.52780.19843920.165670967488100
6.5278-54.88730.1624070.15317307771499
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.0971-0.1162-0.0452.6052-1.06442.26590.008-0.1503-0.08690.0847-0.04270.0628-0.10810.06390.07830.3592-0.0109-0.07120.30860.07550.367610.5911-5.966-5.2275
20.3196-0.00720.08910.78280.08630.13960.0875-0.21850.02080.2319-0.1665-0.143-0.07920.111-0.01940.4913-0.0518-0.06770.39240.00820.26673.651912.75399.6128
30.79410.05310.40130.60490.06230.68650.0569-0.20050.15810.2931-0.06160.0078-0.08040.02020.01980.4947-0.04570.03010.3258-0.01210.2821-10.806114.43779.0585
41.1764-0.1471-0.01280.8403-0.20030.82360.07690.0550.21410.0524-0.03580.2066-0.0911-0.1849-0.06050.3644-0.00630.04050.31960.03430.3493-28.320118.0492-6.8825
51.62040.65770.17070.40670.14980.6858-0.0356-0.0312-0.08670.01150.0443-0.0470.11710.1365-0.00290.24550.0278-0.02440.30010.07440.2658-15.3888-45.3631-13.1449
60.52370.08450.14071.02250.33521.41440.0157-0.0367-0.2290.0653-0.01290.03830.34410.0511-0.00430.3325-0.00120.00940.23640.06140.357-34.553-61.255-20.0703
70.5062-0.0862-0.0150.5489-0.0080.5855-0.0048-0.0603-0.02840.02060.00720.05890.0239-0.04420.00420.1959-0.00460.00480.21360.02940.214-35.3749-35.5924-27.0012
81.8214-0.76490.43751.5788-0.42661.2218-0.03860.04930.1844-0.1163-0.02590.1316-0.1713-0.11190.05040.2313-0.0048-0.0580.25610.02250.2705-51.4435-19.3339-39.8945
93.0479-2.15291.9593.7782-2.35233.3454-0.1508-0.2187-0.07560.1110.1590.3293-0.2425-0.6022-0.00290.23340.0532-0.04990.4519-0.01050.3808-67.3219-17.0555-40.4309
100.37970.3793-0.14430.9511-0.33410.60.01580.0601-0.1691-0.0105-0.0003-0.2386-0.00960.1737-0.00330.2390.00070.0290.2481-0.03130.319418.252-28.6779-55.6375
110.9-0.0882-0.28020.61540.03980.5264-0.02650.12490.0023-0.12030.0415-0.1628-0.05170.1385-0.01050.2551-0.0430.03040.2455-0.02960.26817.8089-8.9583-64.0225
122.28530.74910.15451.67181.05890.7931-0.12680.3530.1323-0.32790.12580.1243-0.093-0.0801-0.02630.314-0.00530.00190.252-0.00610.20881.9379-13.9321-69.3948
130.58770.0110.06321.0796-0.26530.4586-0.0131-0.04020.04230.06990.03430.0396-0.0699-0.0075-0.00220.2251-0.01060.01850.1774-0.01820.21881.7125-4.9253-52.9349
141.1909-0.44860.2862.1766-0.17240.8976-0.10870.11960.1344-0.39570.11690.5537-0.1197-0.12730.00490.3623-0.0305-0.10630.25940.06850.4685-18.303913.378-68.6287
151.3311-0.5570.18931.57731.48774.7792-0.0772-0.0231-0.31520.03550.0843-0.19520.17460.32530.02260.35980.0074-0.03720.35230.03080.371218.9944-6.3315-26.1387
162.5144-0.1913-0.02170.79270.90854.4883-0.03380.1374-0.2766-0.1885-0.026-0.3063-0.11340.28610.07820.4073-0.0002-0.01590.29330.01290.297719.0486-1.835-30.4452
170.7774-0.12540.11910.6722-0.31251.17750.0366-0.02880.0484-0.011-0.0391-0.1207-0.05010.1951-0.00520.3202-0.0594-0.00870.25490.04020.28679.468224.1422-25.7501
181.6981-0.1435-0.53440.7757-0.71120.97680.13330.10820.0957-0.0377-0.0505-0.0851-0.05230.0336-0.05930.3913-0.00260.00920.28490.05710.28812.205225.5485-35.8367
190.9597-0.3044-0.10480.9232-0.4261.23180.04730.0287-0.0152-0.00170.00750.04720.0697-0.0866-0.03280.3627-0.00380.00010.27290.03390.2475-3.615318.0816-26.9235
201.40771.4504-0.66493.0403-0.57350.3208-0.09930.3680.0401-0.22940.1320.761-0.2099-0.13840.1480.43110.10430.03730.42660.07240.5806-27.415831.9305-30.7893
211.3435-1.70311.63413.0137-0.68814.29020.20750.0933-0.0756-0.20820.04320.95320.069-0.5083-0.11980.40210.0825-0.00280.51250.17240.6613-31.038135.8932-37.4832
221.3914-0.23730.79022.4687-0.5540.59630.0965-0.04640.37080.269-0.04680.3488-0.4357-0.2573-0.03280.48440.12630.09730.32140.09750.4695-19.122143.2674-29.9191
231.73491.39610.34712.54920.77041.62120.1472-0.0637-0.13630.1999-0.1249-0.2975-0.0236-0.0704-0.11220.28250.0026-0.06680.3140.10810.357-2.285-25.62033.4931
241.80131.93120.50482.19640.21081.12510.0660.08110.1070.01780.022-0.0282-0.1024-0.07-0.10060.27750.0337-0.03060.29620.12090.3904-4.6329-23.86242.4564
251.3647-0.9854-1.40793.87511.15253.560.0623-0.47040.13610.7648-0.1129-0.40780.03930.17930.10460.4243-0.074-0.05380.49410.06150.2606-24.8168-20.386215.6072
260.7178-0.05890.06090.9269-0.05690.43130.0382-0.14420.00910.15990.00480.1873-0.0566-0.04910.00370.2551-0.01620.0560.29780.0270.2602-39.8884-18.3955-0.5155
271.08820.2469-0.24371.1347-0.0580.60240.0599-0.13350.05090.1926-0.0251-0.0652-0.05550.0172-0.00980.2764-0.02280.00580.30720.04080.235-27.4054-17.66170.1402
280.9487-0.35910.11360.81390.04650.75220.0204-0.00750.0736-0.1378-0.0020.1889-0.0577-0.0843-0.01230.2416-0.00570.00110.24720.03060.2499-40.422-12.6754-11.2411
291.4451-0.02311.20991.3231-0.44032.712-0.0179-0.06240.2094-0.18130.04070.393-0.2918-0.344-0.05870.31940.08720.00940.39920.04710.5662-57.193-5.5024-17.1445
300.9461-0.164-0.38031.75370.25462.2702-0.0863-0.187-0.31060.02970.0168-0.07820.22490.09640.10810.22650.01770.0030.21920.03660.3032-1.4185-48.8867-34.5655
311.232-0.0487-0.10110.56620.18140.577-0.00090.1027-0.1651-0.07460.02510.05150.1194-0.0809-0.01210.2505-0.0073-0.0070.2185-0.02590.22-20.4206-37.3141-58.0871
320.6582-0.0580.15780.571-0.08920.75520.0080.02280.0141-0.06040.0247-0.0187-0.0591-0.0344-0.03130.2087-0.00480.02860.1817-0.01140.2003-15.5996-27.217-52.8036
331.4093-0.0233-0.34132.08890.15840.88260.08820.15210.398-0.1968-0.03610.3406-0.3307-0.303-0.02170.42190.1219-0.03630.41560.06280.4016-32.4989-7.0926-65.1598
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 0 through 63 )A0 - 63
2X-RAY DIFFRACTION2chain 'A' and (resid 64 through 134 )A64 - 134
3X-RAY DIFFRACTION3chain 'A' and (resid 135 through 284 )A135 - 284
4X-RAY DIFFRACTION4chain 'A' and (resid 285 through 587 )A285 - 587
5X-RAY DIFFRACTION5chain 'B' and (resid 0 through 90 )B0 - 90
6X-RAY DIFFRACTION6chain 'B' and (resid 91 through 192 )B91 - 192
7X-RAY DIFFRACTION7chain 'B' and (resid 193 through 426 )B193 - 426
8X-RAY DIFFRACTION8chain 'B' and (resid 427 through 514 )B427 - 514
9X-RAY DIFFRACTION9chain 'B' and (resid 515 through 591 )B515 - 591
10X-RAY DIFFRACTION10chain 'C' and (resid 1 through 134 )C1 - 134
11X-RAY DIFFRACTION11chain 'C' and (resid 135 through 248 )C135 - 248
12X-RAY DIFFRACTION12chain 'C' and (resid 249 through 284 )C249 - 284
13X-RAY DIFFRACTION13chain 'C' and (resid 285 through 477 )C285 - 477
14X-RAY DIFFRACTION14chain 'C' and (resid 478 through 587 )C478 - 587
15X-RAY DIFFRACTION15chain 'D' and (resid 3 through 28 )D3 - 28
16X-RAY DIFFRACTION16chain 'D' and (resid 29 through 77 )D29 - 77
17X-RAY DIFFRACTION17chain 'D' and (resid 78 through 138 )D78 - 138
18X-RAY DIFFRACTION18chain 'D' and (resid 139 through 217 )D139 - 217
19X-RAY DIFFRACTION19chain 'D' and (resid 218 through 364 )D218 - 364
20X-RAY DIFFRACTION20chain 'D' and (resid 365 through 389 )D365 - 389
21X-RAY DIFFRACTION21chain 'D' and (resid 390 through 411 )D390 - 411
22X-RAY DIFFRACTION22chain 'D' and (resid 412 through 455 )D412 - 455
23X-RAY DIFFRACTION23chain 'E' and (resid 1 through 38 )E1 - 38
24X-RAY DIFFRACTION24chain 'E' and (resid 39 through 77 )E39 - 77
25X-RAY DIFFRACTION25chain 'E' and (resid 78 through 106 )E78 - 106
26X-RAY DIFFRACTION26chain 'E' and (resid 107 through 187 )E107 - 187
27X-RAY DIFFRACTION27chain 'E' and (resid 188 through 296 )E188 - 296
28X-RAY DIFFRACTION28chain 'E' and (resid 297 through 364 )E297 - 364
29X-RAY DIFFRACTION29chain 'E' and (resid 365 through 455 )E365 - 455
30X-RAY DIFFRACTION30chain 'F' and (resid 0 through 77 )F0 - 77
31X-RAY DIFFRACTION31chain 'F' and (resid 78 through 217 )F78 - 217
32X-RAY DIFFRACTION32chain 'F' and (resid 218 through 364 )F218 - 364
33X-RAY DIFFRACTION33chain 'F' and (resid 365 through 455 )F365 - 455

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