+
Open data
-
Basic information
Entry | Database: PDB / ID: 5z6n | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of Escherichia coli ElaA | ||||||
![]() | Protein ElaA | ||||||
![]() | TRANSFERASE / N-acetyltransferase / acetyl coenzyme A | ||||||
Function / homology | ![]() N-acetyltransferase activity / acyltransferase activity, transferring groups other than amino-acyl groups Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Shi, L.L. / Gao, Z.Q. / Zhang, H. / Dong, Y.H. | ||||||
![]() | ![]() Title: Crystal structure of Escherichia coli ElaA Authors: Shi, L.L. / Gao, Z.Q. / Zhang, H. / Dong, Y.H. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 130.7 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 102 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 438.9 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 448.8 KB | Display | |
Data in XML | ![]() | 14.1 KB | Display | |
Data in CIF | ![]() | 18.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5jphS S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 17437.648 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: K12 / Gene: elaA, yfbC, b2267, JW2262 / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.83 Å3/Da / Density % sol: 56.61 % |
---|---|
Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 7.3 / Details: 0.2M Potassium fluoride, 24%(w/v) PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Jul 20, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→50 Å / Num. obs: 14550 / % possible obs: 94.6 % / Redundancy: 5.8 % / CC1/2: 0.983 / Rmerge(I) obs: 0.076 / Rpim(I) all: 0.035 / Rrim(I) all: 0.084 / Net I/σ(I): 25.1 |
Reflection shell | Resolution: 2.4→2.44 Å / Redundancy: 5.7 % / Rmerge(I) obs: 0.336 / Mean I/σ(I) obs: 4.3 / Num. unique obs: 691 / CC1/2: 0.95 / Rpim(I) all: 0.15 / Rrim(I) all: 0.37 / % possible all: 97.6 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 5JPH Resolution: 2.6→30.046 Å / SU ML: 0.3 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 29.86 / Stereochemistry target values: ML
| ||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→30.046 Å
| ||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
| ||||||||||||||||||||||||||||||||||||||||
Refinement TLS params. | Method: refined / Origin x: -3.6384 Å / Origin y: 3.4762 Å / Origin z: 176.1152 Å
| ||||||||||||||||||||||||||||||||||||||||
Refinement TLS group | Selection details: all |