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- PDB-5yuo: Crystal structure of SSB protein from Pseudomonas aeruginosa PAO1 -
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Open data
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Basic information
Entry | Database: PDB / ID: 5yuo | ||||||
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Title | Crystal structure of SSB protein from Pseudomonas aeruginosa PAO1 | ||||||
![]() | Single-stranded DNA-binding protein | ||||||
![]() | DNA BINDING PROTEIN / single-strand DNA binding protein / Pseudomonas aeruginosa | ||||||
Function / homology | ![]() nucleoid / single-stranded DNA binding / DNA recombination / DNA replication / DNA repair Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Huang, Y.H. / Huang, C.Y. | ||||||
![]() | ![]() Title: The glycine-rich flexible region in SSB is crucial for PriA stimulation. Authors: Huang, Y.H. / Huang, C.Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 95.4 KB | Display | ![]() |
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PDB format | ![]() | 72.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1eygS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 13774.341 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 Gene: ssb, PA4232 / Production host: ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.66 Å3/Da / Density % sol: 53.73 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 14% PEG 4000, 100mM HEPES pH 7.0, 50mM sodium acetate |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Mar 2, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.975 Å / Relative weight: 1 |
Reflection | Resolution: 2.037→30 Å / Num. obs: 36409 / % possible obs: 98.2 % / Redundancy: 3.6 % / Rmerge(I) obs: 0.038 / Net I/σ(I): 31.36 |
Reflection shell | Resolution: 2.04→2.11 Å / Rmerge(I) obs: 0.456 / Mean I/σ(I) obs: 2.53 / Num. unique obs: 3312 / % possible all: 89.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1EYG Resolution: 2.037→26.49 Å / SU ML: 0.26 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 30.44
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.037→26.49 Å
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Refine LS restraints |
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LS refinement shell |
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