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- PDB-5yiw: Caulobacter crescentus GcrA DNA-binding domain (DBD) in complex w... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5yiw | ||||||
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Title | Caulobacter crescentus GcrA DNA-binding domain (DBD) in complex with methylated dsDNA (crystal form 2) | ||||||
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![]() | DNA BINDING PROTEIN/DNA / Caulobacter crescentus / GcrA / DNA-binding / transcription factor / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex | ||||||
Function / homology | GcrA cell cycle regulator / GcrA cell cycle regulator / metal ion binding / (R,R)-2,3-BUTANEDIOL / DNA / DNA (> 10) / Cell cycle regulatory protein GcrA![]() | ||||||
Biological species | ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Wu, X. / Zhang, Y. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural insights into the unique mechanism of transcription activation by Caulobacter crescentus GcrA. Authors: Wu, X. / Haakonsen, D.L. / Sanderlin, A.G. / Liu, Y.J. / Shen, L. / Zhuang, N. / Laub, M.T. / Zhang, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 57.4 KB | Display | ![]() |
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PDB format | ![]() | 37.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5yiuSC ![]() 5yivC ![]() 5yixC ![]() 5z7iC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein/peptide | Mass: 5317.199 Da / Num. of mol.: 3 / Fragment: DNA-binding domain (DBD) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: NA1000 / CB15N / Gene: gcrA, CCNA_02328 / Plasmid: pET28a / Production host: ![]() ![]() #2: DNA chain | | Mass: 3299.174 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #3: DNA chain | | Mass: 3397.250 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #4: Chemical | ChemComp-BU3 / ( | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.21 Å3/Da / Density % sol: 44.36 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / Details: 20% PEG 8000, 0.05M Potassium phosphate monobasic |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: May 8, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 1.55→50 Å / Num. obs: 29530 / % possible obs: 99.6 % / Redundancy: 7.2 % / Biso Wilson estimate: 19.46 Å2 / Rmerge(I) obs: 0.07 / Rpim(I) all: 0.03 / Rrim(I) all: 0.07 / Rsym value: 0.07 / Χ2: 0.95 / Net I/σ(I): 22.5 |
Reflection shell | Resolution: 1.55→1.58 Å / Redundancy: 6.9 % / Rmerge(I) obs: 0.74 / Mean I/σ(I) obs: 2.5 / Num. unique obs: 1394 / CC1/2: 0.88 / Rpim(I) all: 0.3 / Rrim(I) all: 0.8 / Rsym value: 0.74 / Χ2: 0.95 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5YIU Resolution: 1.551→28.745 Å / SU ML: 0.15 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 25.68
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 27.7 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.551→28.745 Å
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Refine LS restraints |
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LS refinement shell |
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