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Structure paper

TitleStructural insights into the unique mechanism of transcription activation by Caulobacter crescentus GcrA.
Journal, issue, pagesNucleic Acids Res., Vol. 46, Page 3245-3256, Year 2018
Publish dateOct 6, 2017 (structure data deposition date)
AuthorsWu, X. / Haakonsen, D.L. / Sanderlin, A.G. / Liu, Y.J. / Shen, L. / Zhuang, N. / Laub, M.T. / Zhang, Y.
External linksNucleic Acids Res. / PubMed:29514271
MethodsX-ray diffraction
Resolution1.42 - 2.914 Å
Structure data

PDB-5yiu:
Caulobacter crescentus GcrA DNA-binding domain (DBD)
Method: X-RAY DIFFRACTION / Resolution: 1.42 Å

PDB-5yiv:
Caulobacter crescentus GcrA DNA-binding domain(DBD) in complex with methylated dsDNA(crystal form 1)
Method: X-RAY DIFFRACTION / Resolution: 2.914 Å

PDB-5yiw:
Caulobacter crescentus GcrA DNA-binding domain (DBD) in complex with methylated dsDNA (crystal form 2)
Method: X-RAY DIFFRACTION / Resolution: 1.551 Å

PDB-5yix:
Caulobacter crescentus GcrA sigma-interacting domain (SID) in complex with domain 2 of sigma 70
Method: X-RAY DIFFRACTION / Resolution: 2.302 Å

PDB-5z7i:
Caulobacter crescentus GcrA DNA-binding domain(DBD)in complex with unmethylated dsDNA
Method: X-RAY DIFFRACTION / Resolution: 1.601 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-BU3:
(R,R)-2,3-BUTANEDIOL

ChemComp-ZN:
Unknown entry

Source
  • caulobacter crescentus (strain na1000 / cb15n) (bacteria)
  • synthetic construct (others)
  • caulobacter crescentus (bacteria)
KeywordsDNA BINDING PROTEIN / Caulobacter crescentus / GcrA / DNA-binding / transcription factor / DNA BINDING PROTEIN/DNA / DNA-binding domain / DNA BINDING PROTEIN-DNA complex / sigma-interacting domain

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