PDB-5xv4: Crystal structure of ATG101-ATG13HORMA

Basic information

• Entry: Database: PDB / ID: 5xv4
• Title: Crystal structure of ATG101-ATG13HORMA

Components

• Autophagy-related protein 101
• Autophagy-related protein 13

• Keywords: PROTEIN BINDING / AUTOPHAGY / ATG101 / ATG13 / HORMA

Function / homology

Function:

regulation of protein lipidation / Atg1/ULK1 kinase complex / response to mitochondrial depolarisation / protein localization to phagophore assembly site / phagophore assembly site membrane / piecemeal microautophagy of the nucleus / protein kinase regulator activity / phagophore assembly site / positive regulation of protein targeting to mitochondrion / Macroautophagy / mitophagy / autophagosome assembly / autophagosome / positive regulation of autophagy / protein serine/threonine kinase activator activity / negative regulation of cell population proliferation / protein-containing complex binding / endoplasmic reticulum membrane / protein kinase binding / mitochondrion / identical protein binding / cytoplasm / cytosol

Domain/homology:

Autophagy-related protein 101 / Autophagy-related protein 101 / Autophagy-related protein 13, N-terminal / Autophagy-related protein 13 / Autophagy-related protein 13 / Cell Cycle, Spindle Assembly Checkpoint Protein; Chain A / HORMA domain / HORMA domain superfamily / 2-Layer Sandwich / Alpha Beta

Component:

Autophagy-related protein 13 / Autophagy-related protein 101

• Biological species: Homo sapiens (human)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.95 Å
• Authors: Kim, B.-W. / Song, H.K.
• Citation: Journal: Autophagy / Year: 2018; Title: The C-terminal region of ATG101 bridges ULK1 and PtdIns3K complex in autophagy initiation.; Authors: Kim, B.-W. / Jin, Y. / Kim, J. / Kim, J.H. / Jung, J. / Kang, S. / Kim, I.Y. / Kim, J. / Cheong, H. / Song, H.K.

History

Deposition	Jun 26, 2017	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	Jul 4, 2018	Provider: repository / Type: Initial release
Revision 1.1	Jan 16, 2019	Group: Data collection / Database references / Category: citation / citation_author Item: _citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2	Nov 22, 2023	Group: Data collection / Database references / Refinement description Category: chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

Assembly

Deposited unit

A: Autophagy-related protein 13

B: Autophagy-related protein 101

C: Autophagy-related protein 13

D: Autophagy-related protein 101

E: Autophagy-related protein 13

F: Autophagy-related protein 101

G: Autophagy-related protein 13

H: Autophagy-related protein 101

I: Autophagy-related protein 13

J: Autophagy-related protein 101

K: Autophagy-related protein 13

L: Autophagy-related protein 101

M: Autophagy-related protein 13

N: Autophagy-related protein 101

O: Autophagy-related protein 13

P: Autophagy-related protein 101

Summary:

• 369 kDa, 16 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	369,457	16
Polymers	369,457	16
Non-polymers	0	0
Water	0	0

1

A: Autophagy-related protein 13

B: Autophagy-related protein 101

Summary:

• defined by author&software
• Evidence: gel filtration, 1:1 hetero dimer, SAXS, 1:1 hetero dimer
• 46.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	46,182	2
Polymers	46,182	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	2550 Å2
ΔGint	-12 kcal/mol
Surface area	18780 Å2
Method	PISA

2

C: Autophagy-related protein 13

D: Autophagy-related protein 101

Summary:

• defined by author&software
• 46.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	46,182	2
Polymers	46,182	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	2530 Å2
ΔGint	-11 kcal/mol
Surface area	19460 Å2
Method	PISA

3

E: Autophagy-related protein 13

F: Autophagy-related protein 101

Summary:

• defined by author&software
• 46.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	46,182	2
Polymers	46,182	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	2480 Å2
ΔGint	-14 kcal/mol
Surface area	18950 Å2
Method	PISA

4

G: Autophagy-related protein 13

H: Autophagy-related protein 101

Summary:

• defined by author&software
• 46.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	46,182	2
Polymers	46,182	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	2610 Å2
ΔGint	-11 kcal/mol
Surface area	19450 Å2
Method	PISA

5

I: Autophagy-related protein 13

J: Autophagy-related protein 101

Summary:

• defined by author&software
• 46.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	46,182	2
Polymers	46,182	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	2600 Å2
ΔGint	-13 kcal/mol
Surface area	19120 Å2
Method	PISA

6

K: Autophagy-related protein 13

L: Autophagy-related protein 101

Summary:

• defined by author&software
• 46.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	46,182	2
Polymers	46,182	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	2560 Å2
ΔGint	-12 kcal/mol
Surface area	19290 Å2
Method	PISA

7

M: Autophagy-related protein 13

N: Autophagy-related protein 101

Summary:

• defined by author&software
• 46.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	46,182	2
Polymers	46,182	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	2580 Å2
ΔGint	-12 kcal/mol
Surface area	19700 Å2
Method	PISA

8

O: Autophagy-related protein 13

P: Autophagy-related protein 101

Summary:

• defined by author&software
• 46.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	46,182	2
Polymers	46,182	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	2520 Å2
ΔGint	-12 kcal/mol
Surface area	19320 Å2
Method	PISA

Unit cell

Length a, b, c (Å)	101.287, 125.356, 174.618
Angle α, β, γ (deg.)	90.000, 95.690, 90.000
Int Tables number	3
Space group name H-M	P121

Components

#1: Protein

A C E G I K M O
Autophagy-related protein 13

Details:

Mass: 21319.652 Da / Num. of mol.: 8 / Fragment: UNP residues 1-190
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ATG13, KIAA0652 / Production host: Escherichia coli (E. coli) / References: UniProt: O75143

#2: Protein

B D F H J L N P
Autophagy-related protein 101

Details:

Mass: 24862.424 Da / Num. of mol.: 8 / Mutation: K40A/K41A/E42A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ATG101, C12orf44, PP894 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9BSB4

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 2.97 ų/Da / Density % sol: 58.65 %
• Crystal grow: Temperature: 293 K / Method: vapor diffusion, hanging drop; Details: 0.1M HEPES (pH7.5), 0.2M MgCl2, 6-8% (w/v) PEG 3350

Data collection

• Diffraction: Mean temperature: 100 K
• Diffraction source: Source: SYNCHROTRON / Site: Photon Factory / Beamline: AR-NE3A / Wavelength: 1 Å
• Detector: Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jun 4, 2015
• Radiation: Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 1 Å / Relative weight: 1
• Reflection: Resolution: 2.95→50 Å / Num. obs: 89076 / % possible obs: 97.6 % / Redundancy: 3.6 % / R_merge(I) obs: 0.089 / R_pim(I) all: 0.054 / R_rim(I) all: 0.104 / Χ²: 1.124 / Net I/av σ(I): 16.467 / Net I/σ(I): 5.6 / Num. measured all: 317835

Reflection shell

Diffraction-ID: 1 / Rejects: _

Resolution (Å)	Redundancy (%)	Rmerge(I) obs	Num. unique all	CC1/2	Rpim(I) all	Rrim(I) all	Χ2	% possible all
2.95-3	3.4	0.579	4283	0.956	0.355	0.682	0.567	94.4
3-3.06	3.6	0.504	4299	0.967	0.3	0.589	0.567	94.4
3.06-3.11	3.6	0.43	4341	0.975	0.257	0.503	0.561	95.6
3.11-3.18	3.6	0.366	4297	0.976	0.218	0.428	0.607	95
3.18-3.25	3.5	0.296	4384	0.984	0.178	0.347	0.669	96.2
3.25-3.32	3.5	0.227	4355	0.988	0.136	0.266	0.716	96.3
3.32-3.41	3.5	0.204	4407	0.99	0.124	0.24	0.715	96.5
3.41-3.5	3.5	0.185	4434	0.988	0.112	0.218	0.803	96.8
3.5-3.6	3.5	0.148	4387	0.992	0.089	0.174	0.768	97
3.6-3.72	3.5	0.123	4439	0.994	0.075	0.145	0.933	97.1
3.72-3.85	3.4	0.113	4432	0.993	0.07	0.133	1.019	97.9
3.85-4	3.2	0.099	4430	0.994	0.065	0.119	1.288	97.7
4-4.19	3.4	0.086	4547	0.995	0.055	0.103	1.284	99
4.19-4.41	3.4	0.079	4514	0.995	0.05	0.094	1.541	99.2
4.41-4.68	3.8	0.076	4569	0.996	0.046	0.089	1.585	99.8
4.68-5.04	3.9	0.074	4562	0.995	0.044	0.086	1.657	99.8
5.04-5.55	3.9	0.068	4570	0.996	0.041	0.079	1.503	99.7
5.55-6.35	3.8	0.075	4566	0.995	0.045	0.087	1.505	99.8
6.35-8	3.4	0.06	4611	0.996	0.038	0.071	1.662	99.8
8-50	3.8	0.048	4649	0.997	0.028	0.056	2.047	98.9

Processing

Software

Name	Version	Classification
HKL-2000		data scaling
PHENIX	1.10.1_2155	refinement
PDB_EXTRACT	3.2	data extraction

Refinement

Method to determine structure: MOLECULAR REPLACEMENT
Starting model: 5XUY

5xuy

Resolution: 2.95→35.782 Å / SU ML: 0.47 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 43.79

	Rfactor	Num. reflection	% reflection
Rfree	0.3128	1959	2.24 %
Rwork	0.2861	-	-
obs	0.2867	87494	95.32 %

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
• Displacement parameters: B_iso max: 150.09 Ų / B_iso mean: 76.7525 Ų / B_iso min: 41.37 Ų

Refinement step

Cycle: final / Resolution: 2.95→35.782 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	23827	0	0	0	23827
Num. residues	-	-	-	-	3124

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.003	24231
X-RAY DIFFRACTION	f_angle_d	0.585	32872
X-RAY DIFFRACTION	f_chiral_restr	0.042	3884
X-RAY DIFFRACTION	f_plane_restr	0.003	4165
X-RAY DIFFRACTION	f_dihedral_angle_d	15.123	14494

LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 14

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Num. reflection all	% reflection obs (%)
2.9473-3.021	0.4349	125	0.407	5475	5600	86
3.021-3.1026	0.4054	139	0.3763	5979	6118	93
3.1026-3.1938	0.3747	138	0.3725	6005	6143	94
3.1938-3.2969	0.3675	140	0.355	6060	6200	95
3.2969-3.4146	0.3137	139	0.3327	6009	6148	95
3.4146-3.5512	0.3568	138	0.3309	6049	6187	95
3.5512-3.7127	0.358	140	0.3105	6140	6280	95
3.7127-3.9082	0.3584	142	0.2952	6183	6325	97
3.9082-4.1527	0.2975	139	0.2742	6166	6305	97
4.1527-4.4728	0.2886	138	0.2679	6238	6376	97
4.4728-4.9219	0.2551	147	0.2535	6274	6421	98
4.9219-5.6317	0.3255	147	0.2577	6368	6515	99
5.6317-7.0863	0.3368	146	0.2777	6303	6449	98
7.0863-35.785	0.219	141	0.2224	6286	6427	96

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	3.4114	-1.324	-0.0738	2.8324	1.5845	4.749	0.1369	0.0483	-0.3156	-0.4209	-0.3205	-0.9888	-0.4685	0.0779	-0.0485	0.4598	-0.1369	0.2966	0.3584	-0.081	0.9852	-80.7601	6.1152	61.1263
2	4.5586	-1.2024	-0.4552	4.9322	2.7259	4.8976	0.0459	-1.1301	-1.0494	0.7414	0.0996	-0.504	0.5899	-0.4686	0.2347	0.4464	-0.053	0.0323	0.8162	0.0957	1.1445	-89.3234	5.5963	78.6575
3	6.6787	-5.8296	-0.4781	7.6827	0.546	1.2413	-0.2574	-0.8357	-0.4288	1.2308	-0.2424	-0.6864	-0.0681	0.0638	-0.0393	0.6566	-0.1505	0.2077	0.3967	-0.0637	1.0439	-81.8935	12.4956	70.6359
4	2.9266	-0.9997	0.2424	3.3477	-0.8912	3.3605	0.2126	0.2521	-1.2092	-0.0497	-0.2949	-0.0129	0.3727	0.5526	0.05	0.509	0.0047	0.2006	0.689	-0.096	1.518	-77.043	-3.2416	60.7161
5	7.8173	5.48	-3.4396	3.9337	-2.9918	5.2422	-0.1368	-0.9132	-2.2631	-0.0715	-0.3847	-0.5731	2.2263	0.2739	0.6028	0.9661	-0.1148	0.2208	0.632	0.039	1.6564	-89.441	-17.2348	69.7432
6	2.1239	-0.659	-0.0199	0.4549	0.6805	2.7802	0.1154	0.7154	0.1108	-0.4685	-0.1428	0.0178	-0.7182	-0.3554	0.0313	0.8487	0.0185	-0.0074	0.5709	-0.1903	1.0432	-87.2876	5.0371	52.5024
7	2.7437	1.1461	-1.0751	4.3354	-0.8946	4.2151	0.1509	-0.0957	-0.3807	0.0835	-0.1003	0.4218	0.0032	-0.3921	-0.0329	0.4413	-0.0231	0.1844	0.365	-0.0319	1.1634	-93.1775	3.6227	66.2093
8	3.2191	0.1485	0.5227	3.5892	-0.0189	0.0853	-0.2904	-0.5374	-1.3281	0.6937	-0.0579	-0.075	0.6493	-0.1315	0.2703	0.7242	-0.1787	0.3045	0.5763	0.1706	1.4825	-86.877	-12.2154	73.6533
9	1.0155	-0.0689	-0.6861	0.4385	-0.4151	0.962	-0.6019	0.3742	-0.4526	-1.0365	0.6534	-0.6916	0.0732	-0.2261	0.2197	1.6192	-0.0018	0.161	0.5779	-0.2117	1.5472	-89.5008	-13.4688	56.7437
10	6.0755	4.7314	-3.2783	6.8398	-5.6256	7.33	-0.3704	0.4535	-0.4398	-0.651	0.1579	0.3812	0.3955	-0.2382	0.2855	0.6226	-0.0549	0.1937	0.7527	-0.2247	1.3008	-92.8981	-0.7174	55.8242
11	6.6651	1.0828	0.7493	3.8383	-0.0143	6.2915	-0.1295	-0.1213	1.0107	-0.5248	0.2934	0.8261	0.4109	-0.1915	-0.1836	0.4996	-0.1131	-0.0204	0.6275	-0.2664	1.7176	-112.5123	8.2924	68.8812
12	2.5503	1.4776	0.604	3.4289	0.286	1.8907	0.014	-0.3485	-0.2083	0.0698	0.0525	-0.0328	0.0306	0.0855	-0.0896	0.4917	-0.0777	0.2246	0.3954	-0.1377	1.1231	-96.1013	14.1519	71.8242
13	1.2016	-0.6935	0.7514	2.1435	0.4491	0.9076	0.3852	-0.1436	0.3804	0.5013	-0.2258	1.0386	0.1754	-0.074	-0.2218	0.6639	-0.1108	0.2153	0.6503	-0.1435	1.5856	-112.676	18.7539	70.0677
14	6.9378	3.4381	-3.5018	7.2834	-4.8604	4.0761	-0.284	-0.4131	-0.6902	0.2683	-0.1788	0.4244	0.7088	-0.7198	0.5075	0.7503	-0.1465	0.2305	0.506	-0.0579	1.296	-109.0152	-1.5429	70.0539
15	2.7077	0.6678	0.3394	3.3375	-0.8632	3.0617	-0.3543	0.39	0.9721	-0.2243	-0.3456	-0.1553	-0.0423	0.0555	0.6062	0.6045	-0.0555	0.1951	0.3923	-0.0481	1.4481	-97.967	25.2076	64.8307
16	9.1315	6.0919	5.6401	5.4736	6.566	9.0365	0.0328	-0.0561	0.9182	0.2623	0.2975	1.3387	0.7896	-0.4759	-0.0149	0.8784	-0.1607	-0.0094	0.6972	0.0694	2.2039	-127.1832	8.6278	62.6845
17	3.3087	0.9074	0.1654	7.6879	1.9617	8.2421	-0.0591	0.3857	0.188	-0.6008	0.2369	0.9271	0.3835	-0.3614	-0.1693	0.4539	-0.2112	-0.0867	0.441	0.1818	1.4337	-80.329	46.9727	60.7407
18	2.8767	2.2183	0.3614	3.2246	-0.285	0.604	0.1711	-0.2709	0.4844	0.1727	-0.255	-0.5373	-0.2501	0.2202	0.158	0.8787	-0.5849	0.4452	0.8947	-0.2061	1.2244	-70.8759	47.5388	79.0036
19	4.2201	-1.2755	2.2837	3.1198	0.0098	4.0344	-0.4724	-0.6672	0.8831	0.7886	-0.1254	1.2529	-0.1329	-0.5951	0.1305	0.5453	-0.0452	0.1958	0.5275	0.0011	1.2627	-81.4316	47.4782	69.532
20	2.7688	0.5491	-0.2179	3.1464	-0.2298	1.4133	-0.0884	0.0026	1.6918	-0.4598	0.1671	0.377	-0.9674	-0.1002	0.158	0.8336	-0.0621	-0.0059	0.69	0.217	2.194	-75.585	64.4376	62.8397
21	1.3832	-0.7086	1.0041	0.6114	0.2408	4.6853	0.0369	0.6	0.5625	-0.5301	-0.1599	-0.1314	0.1086	0.2021	0.082	0.7565	-0.0221	0.2456	0.6059	0.2627	1.2695	-73.2309	48.2951	52.8165
22	3.4882	1.5031	0.2004	3.3957	-1.3587	4.4747	0.1883	-0.0999	0.0156	-0.0726	-0.2077	-0.0742	0.0411	0.2112	0.0109	0.5331	-0.0988	0.1949	0.3469	-0.0161	1.0154	-69.1289	44.8362	62.9319
23	2.2729	0.8139	1.8576	2.1099	-0.0092	1.7737	-0.5453	0.2205	1.6558	-0.4786	0.0132	0.6143	-0.6964	0.4053	0.4562	0.7607	-0.1549	0.1499	0.6242	0.1601	1.7459	-68.8902	60.1329	62.8261
24	5.9193	3.2419	2.53	3.4212	0.1158	4.6952	-0.0871	1.1952	-0.6834	-0.8062	0.7831	-0.6713	-0.2143	0.7976	-0.3272	0.4342	0.074	0.2674	0.8683	-0.0003	1.372	-47.9703	42.7533	60.8995
25	3.1692	2.8387	0.6114	5.5872	1.2651	1.9289	0.4046	-0.7047	0.0543	0.5652	-0.487	0.1043	-0.053	-0.0149	-0.0975	0.5207	-0.1386	0.2899	0.5067	0.0644	1.0438	-61.3756	40.7202	73.1503
26	5.1888	0.1553	0.6144	4.7117	1.7397	3.4542	0.5572	-0.9876	-0.8192	1.0637	-0.4008	-1.0268	-0.065	0.3264	-0.1522	0.7067	-0.1641	0.0988	0.814	0.2915	1.2463	-48.0564	37.6554	79.8512
27	1.6564	0.6308	-0.1456	3.8761	0.7167	1.9129	0.1353	0.579	-0.7243	0.406	0.0314	-0.6617	0.1942	0.448	-0.2401	0.5915	0.0106	0.2031	0.7313	0.071	1.5156	-47.6653	33.1273	65.3484
28	8.9055	6.0207	7.7014	8.3345	6.658	8.625	-0.0013	0.3064	0.7432	-0.0939	0.0608	-0.2164	-0.2459	0.7322	-0.0503	0.6329	-0.0906	0.2858	0.4981	-0.0194	1.32	-51.4625	54.8184	70.2601
29	2.8402	0.7972	1.2649	3.7707	2.3313	4.197	-0.0541	0.1788	-1.4026	0.0071	-0.3373	-0.6695	0.7455	-0.0353	0.0663	0.5507	-0.0501	0.1008	0.4192	0.0243	1.3569	-62.3487	28.1406	64.9708
30	6.8225	5.2181	-2.6914	4.0097	-2.1899	1.8627	-0.3972	0.2233	-1.1121	-0.5232	0.5716	-0.5642	0.0238	0.1593	-0.29	0.8818	-0.0187	0.0415	0.7371	-0.2881	2.0133	-33.4291	44.9235	62.756
31	4.5855	-1.535	0.0252	4.8937	1.3336	4.963	0.0278	-0.2763	0.0477	0.631	-0.0477	0.7909	-0.6294	-0.3139	-0.1402	0.5448	0.0845	0.4322	0.3675	0.0553	0.956	-130.4488	68.8534	69.2345
32	3.7861	-0.4731	-0.4526	5.5255	-0.315	2.8058	0.4449	0.857	0.111	-0.3215	-0.3824	0.689	-0.1651	-0.1793	-0.1215	0.3329	-0.0292	0.1252	0.4536	-0.0099	0.9911	-126.5664	72.7429	56.7525
33	3.4084	0.1192	0.7	3.1511	-0.7917	3.9452	0.0165	0.2743	-1.1837	0.2274	-0.0647	0.5415	0.7822	-0.7613	0.0654	0.6274	-0.1582	0.2049	0.4768	0.0037	1.1753	-129.5442	54.2325	66.2399
34	2.6816	0.1358	0.6563	0.5808	-1.5047	4.2894	0.0537	-0.6549	0.1706	0.6898	-0.1663	-0.2474	-0.7834	-0.4045	0.1469	0.906	-0.1455	0.0279	0.4801	0.0376	0.8726	-123.8761	67.7853	77.8424
35	5.13	-2.4058	-1.1327	9.0773	1.803	4.7392	0.075	-0.0483	-0.6782	0.204	-0.2445	-0.8351	0.2112	0.2938	0.1327	0.3561	-0.0344	0.1171	0.3343	0.0611	0.5409	-118.0226	66.327	64.2285
36	7.148	-3.4804	-4.7822	4.6384	3.5208	6.2601	-0.4778	0.354	-1.1148	-0.4538	-0.2461	-0.1474	0.6666	0.1002	0.5199	0.5085	-0.0835	0.2441	0.3542	-0.1673	1.2371	-123.6282	50.264	56.4095
37	1.6473	-0.7478	-0.6793	2.6639	-1.16	1.6141	-0.4993	-0.345	-0.1741	0.8638	0.1987	0.8108	0.1917	0.2435	0.3284	1.0459	-0.1041	0.2673	0.6414	0.3019	1.4746	-122.0869	49.3208	73.4528
38	4.0947	-3.06	-1.8105	5.1824	4.4838	6.4387	-0.0136	-0.4322	-0.5508	0.7867	-0.0234	-0.3984	0.4019	-0.0057	0.0191	0.4634	-0.025	0.1601	0.6591	0.3521	0.9245	-118.4798	62.0373	74.4721
39	5.5925	-3.5969	-1.7366	5.2392	0.5391	4.0299	-0.3689	-0.6928	1.1424	0.8053	0.6497	-0.2922	-0.2729	0.0681	-0.3	0.4628	0.067	0.1101	0.8073	0.1293	1.4776	-98.2013	72.4222	67.8424
40	2.8416	-0.1242	-0.4424	2.2416	-0.8687	2.2345	0.0024	0.1913	0.29	-0.0999	0.0184	0.1019	0.0111	0.0948	-0.005	0.3278	-0.0475	0.1427	0.4215	0.0537	0.834	-112.5453	75.5712	57.6365
41	4.6111	-0.0294	1.2204	2.3066	-0.1963	1.6564	0.5462	1.3071	0.6151	0.0786	-0.6288	-1.1308	0.0522	0.2328	-0.1162	0.5833	0.0559	0.3944	0.7353	0.1713	1.545	-96.6789	77.3614	52.7665
42	1.5814	0.0045	-0.3828	1.3004	-1.3856	1.6686	0.1181	-0.3094	0.5375	0.5588	-0.1061	-0.7784	-0.1312	-0.0276	0.012	0.5158	-0.1939	0.0474	0.632	-0.0332	1.5101	-99.8532	84.5665	66.2793
43	5.1565	-2.7717	-2.2205	7.5391	4.6519	4.9007	-0.2932	0.202	-1.1765	-0.0864	-0.197	-0.7217	1.1001	0.7247	0.3876	0.6488	0.1254	0.2109	0.4826	0.1372	1.239	-100.9404	59.3338	58.0836
44	3.9226	-0.1425	0.4541	2.1379	0.3349	5.8607	-0.0503	-0.29	1.3388	0.1384	-0.5042	-0.3018	-0.4697	-0.1463	0.3183	0.5495	-0.0537	0.1181	0.3351	-0.028	1.2424	-112.8746	86.288	66.4264
45	5.6096	-2.9067	3.7038	3.1368	-1.1687	5.3991	-0.3241	0.0885	0.9306	0.4273	0.2395	-0.9218	0.2519	0.4833	0.0714	0.9163	-0.007	-0.0216	0.7319	-0.1026	2.0549	-84.085	71.2482	67.8296
46	3.9749	-0.3275	1.2398	7.1262	-1.8773	8.0976	0.0501	-0.191	0.7908	0.5735	0.1089	-1.1031	0.3718	0.2824	-0.268	0.3958	0.0227	0.056	0.4976	-0.2606	1.3792	-30.181	-15.3355	69.0201
47	3.157	0.4161	-0.1202	1.2813	-0.4528	4.0052	-0.0384	0.1354	1.0264	-0.1874	-0.1747	-0.577	-0.5893	-0.0044	0.3135	0.5727	-0.0395	0.2088	0.5272	-0.046	1.4721	-30.4972	-13.1556	62.4802
48	2.796	0.0377	0.9	0.9469	1.5042	2.6428	-0.3593	0.5631	1.2974	0.3174	-0.4798	-1.0413	-1.3041	0.5062	0.8339	1.1023	-0.0684	0.0322	0.7351	0.1439	2.6102	-38.7712	8.1427	60.2181
49	2.5442	0.4382	1.0003	0.9171	-1.031	4.9526	-0.1646	-0.5524	0.4097	1.1345	-0.5341	-0.3485	0.5279	-0.368	0.6402	0.994	-0.0935	0.0545	0.6548	-0.2533	1.4582	-36.6942	-14.2468	77.5444
50	2.5219	-0.6805	2.2454	2.5709	-1.6367	3.0406	-0.2677	-0.5233	1.1693	0.4114	0.1743	-0.3421	-0.623	-0.458	0.2343	0.7104	0.0185	0.2512	0.5762	-0.2037	1.6111	-40.998	-6.3744	67.4657
51	6.2056	-1.8756	1.7241	4.1842	-2.4954	8.1615	0.7154	-0.5226	-0.3121	0.1288	0.3337	0.7412	-0.6417	-0.3875	-0.8656	0.3736	-0.0624	0.128	0.7847	-0.2653	1.2832	-62.076	-17.6315	61.2783
52	4.5521	-2.7862	0.2303	3.2269	0.0322	1.6794	0.4435	0.5852	0.0502	-0.3865	-0.3911	-0.4956	-0.0045	-0.0246	-0.1552	0.5587	-0.0254	0.2489	0.3968	-0.0808	1.047	-45.6022	-23.3505	58.3514
53	7.1763	-2.7393	0.314	3.8791	-0.3017	2.5275	0.7921	1.3136	-0.8448	-1.0041	-0.592	0.8149	-0.003	-0.2451	-0.246	0.7475	0.1817	-0.0759	0.756	-0.3047	1.2489	-61.7874	-24.9827	50.378
54	1.5039	0.3071	-0.604	2.1922	-0.1296	1.7719	0.127	-0.3883	-0.6455	0.0027	-0.0627	0.7684	0.3295	-0.2667	-0.1479	0.5946	-0.1749	0.1933	0.6618	-0.1659	1.5207	-62.2575	-29.5286	64.9104
55	5.8733	-2.8433	4.5201	4.6379	-3.1925	7.3246	-0.1371	-0.2721	0.7197	0.4038	0.0887	0.2043	-0.8721	-0.828	-0.0452	0.6056	0.0406	0.3386	0.5548	-0.0926	1.2252	-58.4179	-7.7558	60.0076
56	1.9302	-0.0202	0.7216	5.6925	-0.3103	2.2504	-0.0645	-0.316	-1.4219	-0.0831	-0.2973	0.5586	0.2819	-0.187	0.2059	0.4864	-0.1046	0.0393	0.5368	0.0345	1.508	-46.1356	-34.871	65.9897
57	5.6898	-4.8172	-1.6676	4.191	0.9492	2.2119	-0.055	-0.5281	-1.1199	0.8213	0.1839	0.5934	-0.1095	-0.452	-0.0984	0.7468	-0.1508	0.1177	0.8398	-0.0211	1.5398	-73.7673	-18.354	68.2802
58	2.5099	-0.6923	0.7457	2.4436	-0.3894	4.47	0.199	0.4122	-0.0159	-0.082	-0.2089	0.5955	-0.6102	-0.1122	-0.1514	0.2608	0.1795	0.0708	0.2967	0.0329	1.2059	-119.8161	7.792	19.7323
59	3.5215	-1.9412	0.0895	2.5417	-1.9088	2.3559	-0.034	-0.0798	-0.9839	0.1654	-0.1063	0.1512	0.358	-0.0665	0.0798	0.4292	0.1768	-0.0583	0.357	0.1043	1.7695	-118.7591	-4.0397	26.6845
60	1.8409	-0.2658	0.1373	2.6431	1.1538	1.9115	-0.0098	-0.1229	-1.2918	0.2449	-0.1445	0.3907	0.8636	0.0966	0.0102	0.3605	0.284	-0.0854	0.2035	0.0754	1.6752	-110.7967	-3.2592	23.9461
61	4.7351	-1.738	0.1054	3.5709	-1.2446	0.518	0.1773	0.1002	0.5133	0.5333	0.3693	-0.5213	-0.0742	-0.0096	-0.3525	0.5802	0.2754	-0.0199	0.5942	0.1481	1.2262	-89.6921	8.0867	17.8166
62	1.154	0.2595	0.3806	1.503	0.2843	1.0505	0.4993	0.7484	0.0458	-0.5381	-0.2102	-0.4377	0.0434	0.1274	-0.1297	0.1993	0.6829	0.1832	0.0358	0.2093	1.2652	-99.4817	14.259	12.7155
63	0.77	0.2731	-0.2171	0.7058	-0.9214	1.2197	0.2028	-0.2247	0.7662	-0.2527	-0.0414	-0.7127	-0.1049	0.1344	-0.0984	0.3911	0.124	0.089	0.4774	0.0245	1.583	-91.0412	21.8108	22.5304
64	3.9343	-1.4861	-1.5388	4.4086	1.6787	4.0361	-0.1413	0.0506	-0.2584	-0.0075	0.1405	-0.3447	1.0263	0.7509	-0.2729	0.5342	0.3133	0.0392	0.2205	-0.1518	1.1253	-93.3777	-1.61	16.6546
65	2.1606	-1.0941	0.0106	2.4089	1.105	0.7776	-0.2698	-0.2239	0.6038	0.2524	-0.0488	-0.2495	-0.0071	0.0375	-0.2599	0.4562	0.3473	0.1423	0.1619	0.0922	1.4565	-104.4353	25.0892	21.9558
66	4.4114	-4.8743	2.4634	5.3939	-2.8446	2.544	-0.0026	-0.2388	0.3214	0.2961	0.5815	-0.5579	0.1798	0.2012	-0.4504	0.9759	0.2106	0.0027	0.6672	-0.0147	2.3361	-75.3196	8.5997	23.9995
67	1.1218	0.5758	-0.6434	1.3955	0.8497	1.6454	-0.1087	-0.3351	-0.2586	0.5696	0.099	-0.476	0.4655	0.2973	-0.0573	0.0288	0.7893	-0.4068	-0.0072	-0.0468	0.5305	-21.355	47.0819	25.7251
68	2.3861	-0.5297	0.4271	2.6233	-0.0634	1.1283	0.1056	0.3595	0.386	-0.2644	-0.1261	-0.6333	0.0286	0.3075	0.202	0.1202	0.3149	0.0698	0.3327	0.0843	1.0802	-22.4591	47.1574	14.8647
69	2.3052	0.5111	-0.2353	1.7209	1.8495	2.2683	-0.4007	-0.0506	1.1245	0.0787	-0.0306	-0.8873	-0.5604	0.0836	0.5595	0.4642	0.1558	0.0155	0.2526	-0.0748	1.4134	-25.3136	64.0273	24.1444
70	3.4078	-0.8626	-0.6256	1.6476	1.9793	3.7381	-0.0393	-0.7007	-0.8169	1.0568	-0.2346	-0.0477	1.0819	0.2822	0.0506	0.7319	-0.007	-0.0043	0.2939	-0.1469	0.8991	-27.8251	48.2293	34.1802
71	3.1516	-0.6962	0.6341	2.3426	0.2218	3.3688	-0.0633	-0.0831	0.2988	0.4274	0.1022	0.1628	-0.1964	-0.4287	0.0106	0.2552	0.2022	-0.0268	0.139	0.2671	0.643	-33.8013	49.5438	20.6461
72	5.333	-0.2788	4.119	1.1816	-1.6127	6.0506	-0.2831	0.1922	0.7138	-0.4629	0.3159	0.8891	-0.7267	-0.5625	-0.1973	0.5474	0.0546	-0.125	0.3919	0.187	1.4351	-28.2397	65.7075	12.6816
73	0.3132	0.2769	-0.2531	0.4925	-1.1009	3.2879	-0.6392	-0.1614	-0.0675	0.6847	-0.2608	-0.3377	-0.8582	-1.0381	0.8432	0.7906	0.1677	-0.0796	0.5444	-0.4038	1.3005	-29.8591	66.485	29.7466
74	3.6256	-1.8934	0.5566	5.993	-1.2658	4.19	-0.3241	-0.3838	0.3186	0.9248	0.4977	0.164	-0.2995	-0.1891	-0.1239	0.3496	0.2388	-0.0779	0.4191	-0.4526	0.8725	-33.4134	53.9056	30.8775
75	5.6739	-3.9378	3.3782	3.2305	-1.061	5.4092	-0.3642	-1.0141	-0.7057	0.5987	1.0092	0.3665	-0.1111	-0.9054	-0.1923	0.3325	-0.0609	0.2005	0.8869	-0.2646	1.484	-53.43	43.5561	24.5051
76	1.353	-0.6265	-0.3672	2.5214	0.1836	1.2754	0.1222	0.2456	-0.4917	-0.0551	-0.16	0.2657	0.2075	0.0164	-0.1884	-0.273	0.6203	-0.0118	-0.2754	-0.0821	0.8571	-39.2889	40.3688	14.0719
77	3.6095	-1.1668	0.7629	1.736	-1.2941	2.3029	0.3463	0.4716	0.0699	-0.2639	-0.2526	0.6668	-0.2575	-0.5201	-0.2454	0.2501	0.4178	-0.0345	0.705	-0.3299	1.2859	-53.0705	37.5911	7.2112
78	0.3756	0.1074	-0.1846	1.0451	1.1121	1.4319	-0.0997	0.034	-0.3861	0.2931	0.0909	0.6588	0.4882	-0.2323	-0.1351	0.5346	-0.0103	0.0093	0.3139	0.0628	1.5702	-52.301	26.2045	23.1378
79	3.2322	0.3166	-0.6023	1.546	-0.1669	2.1422	-0.0852	-0.3904	-0.3011	0.0597	0.1385	0.6601	-0.1649	-0.3864	-0.0691	0.2581	0.0232	0.2416	0.4503	-0.1011	1.2315	-54.7554	41.2035	20.0248
80	2.5261	-1.1614	0.763	2.9377	-0.8499	2.6313	-0.0914	0.0007	0.334	-0.1526	0.0377	0.6187	-0.5451	-0.5934	-0.1754	0.1309	0.6141	-0.0723	0.1929	-0.2161	0.879	-49.5827	54.9196	16.7997
81	1.9179	0.4523	0.9279	3.4533	-0.5258	4.3531	-0.749	-0.2514	-0.7636	0.466	0.0036	0.309	0.2336	-0.5563	0.5596	0.5639	0.1422	0.3275	0.7016	0.328	1.4122	-37.1218	26.6729	27.9272
82	2.5004	-0.9359	0.9584	4.7038	-1.9992	0.9837	0.2418	0.2983	-0.6967	-0.751	-0.4649	0.0051	0.4235	0.0275	0.0307	0.4136	0.1547	-0.0492	0.0283	-0.106	0.9674	-37.6227	28.9816	16.7061
83	6.6366	-4.6383	-2.0958	4.1336	1.0133	2.057	-0.176	-0.6232	-0.6837	0.4957	0.4091	0.1665	-0.2018	-0.6512	-0.2184	0.4085	0.2334	0.1464	0.8496	0.0961	1.4453	-64.7815	43.7881	24.5886
84	4.0096	-1.2195	0.1218	2.975	0.6863	2.7255	0.0614	-0.2072	0.0364	-0.2449	0.0162	-0.8676	-0.4435	0.1204	-0.4092	0.0589	-0.066	0.2999	0.2284	0.0217	1.1387	-73.5933	70.8181	23.2412
85	4.4131	-0.856	0.6031	2.2269	1.2459	2.2683	-0.2206	-0.0473	-0.7645	-0.3465	-0.0839	-0.2427	0.1808	0.3412	0.2011	0.355	0.1608	0.1499	0.3287	-0.094	1.3047	-74.8387	58.8324	16.706
86	2.6393	-0.3223	-0.2458	4.5661	0.5682	1.5684	0.2962	0.1385	-0.6783	-0.2382	0.1041	0.4495	-0.0758	-0.2938	-0.1043	0.388	0.1395	0.1375	0.0884	0.0364	0.6132	-84.4403	66.3523	22.7802
87	2.1203	-0.8932	-1.1109	0.7729	-0.2877	2.8694	-0.2298	0.2242	-0.823	-0.3995	-0.1901	-0.3402	0.1393	-0.4203	0.2631	0.4767	0.1463	0.2902	0.3518	-0.23	1.1388	-81.7603	55.6392	17.3591
88	3.0475	1.0473	-0.9513	1.723	0.0349	1.4109	0.045	-0.0776	0.5062	0.042	-0.0905	0.4427	-0.1716	-0.1871	0.0097	0.0732	0.2148	0.1822	0.2491	-0.1323	1.0573	-92.5637	75.0294	27.3525
89	7.5777	0.5506	2.8306	3.4929	1.7145	2.9384	0.4582	-0.8288	-0.1449	0.4492	-0.1987	0.6643	0.1202	-0.5264	0.183	0.3129	0.1005	0.4746	0.4948	-0.2187	1.3137	-105.7795	77.4154	34.1303
90	2.0354	-1.5349	0.3056	2.3465	0.138	1.2693	0.1397	0.2406	0.5593	-0.2528	-0.2808	0.2952	-0.2241	-0.129	-0.0223	0.3787	0.2964	-0.1464	0.5068	0.0307	1.344	-102.5691	84.5108	20.5582
91	6.0976	3.0611	-2.4071	5.8115	-2.5377	6.4373	-0.0505	-0.0497	-0.9452	0.4558	-0.1704	0.5582	0.6246	-0.3268	0.0353	0.4982	-0.0618	0.1991	0.3225	-0.0726	1.1668	-101.0967	59.4689	28.6832
92	0.6848	0.6217	-1.3902	4.4026	-0.3527	3.024	-0.7117	0.448	0.8436	-0.6639	-0.3356	0.9052	0.0411	-0.1232	0.2245	0.5082	0.0251	0.0341	0.5462	0.2332	1.3975	-89.2267	82.0847	13.2203
93	2.9684	-2.2606	0.6999	2.8307	-0.2742	0.3254	-0.435	-0.2101	0.6831	0.2501	-0.0449	0.1114	-0.337	-0.2367	-0.3734	0.2664	0.4168	0.2063	0.117	-0.2102	1.353	-88.2346	89.2417	24.752
94	1.1869	1.8676	-0.4663	3.6821	-1.2734	2.2136	0.1549	0.0636	-0.2233	-0.3475	0.456	-0.0508	0.3509	-0.4888	0.0933	0.3068	0.2447	0.1052	0.4736	-0.0787	1.537	-116.2693	69.9457	17.4767
95	2.3543	-1.3567	0.3142	2.7894	0.2016	1.4604	0.0318	-0.1337	0.3101	0.3043	0.1147	0.5912	0.2219	0.1776	-0.0756	0.2515	0.1596	0.1597	0.1715	0.1001	1.3147	-69.0975	-17.1428	23.7136
96	3.3566	-1.0923	-0.5572	1.3056	-1.0369	2.3489	-0.0928	-0.0801	1.0701	-0.0646	-0.3508	0.0851	-0.1496	-0.2696	0.3019	0.3868	0.1881	0.1624	0.4005	0.1548	1.5792	-68.026	-5.1568	16.8989
97	5.7819	0.7721	2.7729	1.7284	-0.778	4.8332	0.5901	0.2727	0.0798	-0.254	0.0245	-0.5263	0.3962	0.7136	-0.3184	0.3158	0.1044	0.1786	0.2252	-0.0486	0.8668	-60.2932	-17.6975	19.4232
98	1.295	-0.1098	1.1022	2.2512	1.6375	3.4569	-0.0911	0.0734	1.2005	-0.396	-0.2409	-0.3843	-1.0808	0.1808	-0.062	0.6366	0.1662	0.2797	0.2514	0.1823	1.7532	-60.0496	-2.3293	19.3784
99	5.2695	2.3712	0.0835	1.7924	0.0371	2.4525	0.2898	-0.2743	-0.1119	0.1416	-0.0931	-0.1337	-0.3611	0.3195	-0.2546	0.3028	0.1065	0.0664	0.227	0.0876	1.1009	-50.3003	-21.5659	27.6244
100	1.4694	-1.3529	-1.2495	3.3969	-0.3443	2.0644	0.5092	-0.1341	-0.5449	0.0845	-0.4258	-1.2033	-0.0292	0.141	-0.0319	0.439	0.1256	-0.1354	0.4859	-0.0097	1.4744	-39.6723	-31.0505	28.2658
101	2.6423	1.2503	0.8448	3.0363	0.3563	1.6535	0.0794	0.0248	0.1283	0.2213	-0.3069	-0.3488	-0.2645	0.4182	0.0902	0.4884	0.0742	0.1821	0.4012	0.1212	1.1873	-40.6782	-13.2307	25.6203
102	1.5872	-1.6317	-0.1713	2.2719	0.5636	1.4397	-0.0978	0.0884	-0.5304	-0.119	-0.2077	0.041	0.0853	-0.0048	0.1439	0.3976	0.2384	0.0009	0.1625	0.0299	1.4765	-53.7147	-34.4844	21.5988
103	1.5628	1.1586	-0.924	1.457	-0.2893	1.1112	0.286	0.1275	0.1791	-0.2215	0.1687	-0.6676	-0.0557	0.131	-0.4216	0.802	0.3205	0.0708	0.9548	0.1499	2.3571	-24.8258	-18.1216	19.3708

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 7 through 32 )	A	7 - 32
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 33 through 42 )	A	33 - 42
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 43 through 58 )	A	43 - 58
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 59 through 81 )	A	59 - 81
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 82 through 96 )	A	82 - 96
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 97 through 115 )	A	97 - 115
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 116 through 142 )	A	116 - 142
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 143 through 154 )	A	143 - 154
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 155 through 168 )	A	155 - 168
10	X-RAY DIFFRACTION	10	chain 'A' and (resid 169 through 190 )	A	169 - 190
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 1 through 22 )	B	1 - 22
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 23 through 69 )	B	23 - 69
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 70 through 133 )	B	70 - 133
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 134 through 161 )	B	134 - 161
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 162 through 192 )	B	162 - 192
16	X-RAY DIFFRACTION	16	chain 'B' and (resid 193 through 207 )	B	193 - 207
17	X-RAY DIFFRACTION	17	chain 'C' and (resid 6 through 32 )	C	6 - 32
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 33 through 42 )	C	33 - 42
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 43 through 69 )	C	43 - 69
20	X-RAY DIFFRACTION	20	chain 'C' and (resid 70 through 96 )	C	70 - 96
21	X-RAY DIFFRACTION	21	chain 'C' and (resid 97 through 115 )	C	97 - 115
22	X-RAY DIFFRACTION	22	chain 'C' and (resid 116 through 133 )	C	116 - 133
23	X-RAY DIFFRACTION	23	chain 'C' and (resid 134 through 190 )	C	134 - 190
24	X-RAY DIFFRACTION	24	chain 'D' and (resid 1 through 12 )	D	1 - 12
25	X-RAY DIFFRACTION	25	chain 'D' and (resid 13 through 69 )	D	13 - 69
26	X-RAY DIFFRACTION	26	chain 'D' and (resid 70 through 90 )	D	70 - 90
27	X-RAY DIFFRACTION	27	chain 'D' and (resid 91 through 133 )	D	91 - 133
28	X-RAY DIFFRACTION	28	chain 'D' and (resid 134 through 161 )	D	134 - 161
29	X-RAY DIFFRACTION	29	chain 'D' and (resid 162 through 192 )	D	162 - 192
30	X-RAY DIFFRACTION	30	chain 'D' and (resid 193 through 207 )	D	193 - 207
31	X-RAY DIFFRACTION	31	chain 'E' and (resid 7 through 32 )	E	7 - 32
32	X-RAY DIFFRACTION	32	chain 'E' and (resid 33 through 58 )	E	33 - 58
33	X-RAY DIFFRACTION	33	chain 'E' and (resid 59 through 96 )	E	59 - 96
34	X-RAY DIFFRACTION	34	chain 'E' and (resid 97 through 115 )	E	97 - 115
35	X-RAY DIFFRACTION	35	chain 'E' and (resid 116 through 142 )	E	116 - 142
36	X-RAY DIFFRACTION	36	chain 'E' and (resid 143 through 154 )	E	143 - 154
37	X-RAY DIFFRACTION	37	chain 'E' and (resid 155 through 168 )	E	155 - 168
38	X-RAY DIFFRACTION	38	chain 'E' and (resid 169 through 190 )	E	169 - 190
39	X-RAY DIFFRACTION	39	chain 'F' and (resid 1 through 13 )	F	1 - 13
40	X-RAY DIFFRACTION	40	chain 'F' and (resid 14 through 69 )	F	14 - 69
41	X-RAY DIFFRACTION	41	chain 'F' and (resid 70 through 99 )	F	70 - 99
42	X-RAY DIFFRACTION	42	chain 'F' and (resid 100 through 133 )	F	100 - 133
43	X-RAY DIFFRACTION	43	chain 'F' and (resid 134 through 157 )	F	134 - 157
44	X-RAY DIFFRACTION	44	chain 'F' and (resid 158 through 192 )	F	158 - 192
45	X-RAY DIFFRACTION	45	chain 'F' and (resid 193 through 207 )	F	193 - 207
46	X-RAY DIFFRACTION	46	chain 'G' and (resid 7 through 32 )	G	7 - 32
47	X-RAY DIFFRACTION	47	chain 'G' and (resid 33 through 81 )	G	33 - 81
48	X-RAY DIFFRACTION	48	chain 'G' and (resid 82 through 96 )	G	82 - 96
49	X-RAY DIFFRACTION	49	chain 'G' and (resid 97 through 115 )	G	97 - 115
50	X-RAY DIFFRACTION	50	chain 'G' and (resid 116 through 190 )	G	116 - 190
51	X-RAY DIFFRACTION	51	chain 'H' and (resid 1 through 22 )	H	1 - 22
52	X-RAY DIFFRACTION	52	chain 'H' and (resid 23 through 69 )	H	23 - 69
53	X-RAY DIFFRACTION	53	chain 'H' and (resid 70 through 90 )	H	70 - 90
54	X-RAY DIFFRACTION	54	chain 'H' and (resid 91 through 133 )	H	91 - 133
55	X-RAY DIFFRACTION	55	chain 'H' and (resid 134 through 161 )	H	134 - 161
56	X-RAY DIFFRACTION	56	chain 'H' and (resid 162 through 189 )	H	162 - 189
57	X-RAY DIFFRACTION	57	chain 'H' and (resid 190 through 210 )	H	190 - 210
58	X-RAY DIFFRACTION	58	chain 'I' and (resid 7 through 58 )	I	7 - 58
59	X-RAY DIFFRACTION	59	chain 'I' and (resid 59 through 115 )	I	59 - 115
60	X-RAY DIFFRACTION	60	chain 'I' and (resid 116 through 190 )	I	116 - 190
61	X-RAY DIFFRACTION	61	chain 'J' and (resid 1 through 22 )	J	1 - 22
62	X-RAY DIFFRACTION	62	chain 'J' and (resid 23 through 99 )	J	23 - 99
63	X-RAY DIFFRACTION	63	chain 'J' and (resid 100 through 133 )	J	100 - 133
64	X-RAY DIFFRACTION	64	chain 'J' and (resid 134 through 161 )	J	134 - 161
65	X-RAY DIFFRACTION	65	chain 'J' and (resid 162 through 192 )	J	162 - 192
66	X-RAY DIFFRACTION	66	chain 'J' and (resid 193 through 207 )	J	193 - 207
67	X-RAY DIFFRACTION	67	chain 'K' and (resid 7 through 32 )	K	7 - 32
68	X-RAY DIFFRACTION	68	chain 'K' and (resid 33 through 69 )	K	33 - 69
69	X-RAY DIFFRACTION	69	chain 'K' and (resid 70 through 96 )	K	70 - 96
70	X-RAY DIFFRACTION	70	chain 'K' and (resid 97 through 115 )	K	97 - 115
71	X-RAY DIFFRACTION	71	chain 'K' and (resid 116 through 142 )	K	116 - 142
72	X-RAY DIFFRACTION	72	chain 'K' and (resid 143 through 154 )	K	143 - 154
73	X-RAY DIFFRACTION	73	chain 'K' and (resid 155 through 168 )	K	155 - 168
74	X-RAY DIFFRACTION	74	chain 'K' and (resid 169 through 190 )	K	169 - 190
75	X-RAY DIFFRACTION	75	chain 'L' and (resid 1 through 13 )	L	1 - 13
76	X-RAY DIFFRACTION	76	chain 'L' and (resid 14 through 69 )	L	14 - 69
77	X-RAY DIFFRACTION	77	chain 'L' and (resid 70 through 90 )	L	70 - 90
78	X-RAY DIFFRACTION	78	chain 'L' and (resid 91 through 114 )	L	91 - 114
79	X-RAY DIFFRACTION	79	chain 'L' and (resid 115 through 133 )	L	115 - 133
80	X-RAY DIFFRACTION	80	chain 'L' and (resid 134 through 161 )	L	134 - 161
81	X-RAY DIFFRACTION	81	chain 'L' and (resid 162 through 176 )	L	162 - 176
82	X-RAY DIFFRACTION	82	chain 'L' and (resid 177 through 189 )	L	177 - 189
83	X-RAY DIFFRACTION	83	chain 'L' and (resid 190 through 209 )	L	190 - 209
84	X-RAY DIFFRACTION	84	chain 'M' and (resid 6 through 58 )	M	6 - 58
85	X-RAY DIFFRACTION	85	chain 'M' and (resid 59 through 115 )	M	59 - 115
86	X-RAY DIFFRACTION	86	chain 'M' and (resid 116 through 142 )	M	116 - 142
87	X-RAY DIFFRACTION	87	chain 'M' and (resid 143 through 190 )	M	143 - 190
88	X-RAY DIFFRACTION	88	chain 'N' and (resid 1 through 69 )	N	1 - 69
89	X-RAY DIFFRACTION	89	chain 'N' and (resid 70 through 99 )	N	70 - 99
90	X-RAY DIFFRACTION	90	chain 'N' and (resid 100 through 133 )	N	100 - 133
91	X-RAY DIFFRACTION	91	chain 'N' and (resid 134 through 157 )	N	134 - 157
92	X-RAY DIFFRACTION	92	chain 'N' and (resid 158 through 171 )	N	158 - 171
93	X-RAY DIFFRACTION	93	chain 'N' and (resid 172 through 189 )	N	172 - 189
94	X-RAY DIFFRACTION	94	chain 'N' and (resid 190 through 212 )	N	190 - 212
95	X-RAY DIFFRACTION	95	chain 'O' and (resid 7 through 58 )	O	7 - 58
96	X-RAY DIFFRACTION	96	chain 'O' and (resid 59 through 115 )	O	59 - 115
97	X-RAY DIFFRACTION	97	chain 'O' and (resid 116 through 133 )	O	116 - 133
98	X-RAY DIFFRACTION	98	chain 'O' and (resid 134 through 190 )	O	134 - 190
99	X-RAY DIFFRACTION	99	chain 'P' and (resid 1 through 69 )	P	1 - 69
100	X-RAY DIFFRACTION	100	chain 'P' and (resid 70 through 114 )	P	70 - 114
101	X-RAY DIFFRACTION	101	chain 'P' and (resid 115 through 161 )	P	115 - 161
102	X-RAY DIFFRACTION	102	chain 'P' and (resid 162 through 192 )	P	162 - 192
103	X-RAY DIFFRACTION	103	chain 'P' and (resid 193 through 207 )	P	193 - 207