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Yorodumi- PDB-5xs0: Structure of a ssDNA bound to the outer DNA binding site of RAD52 -
+Open data
-Basic information
Entry | Database: PDB / ID: 5xs0 | ||||||||||||||||||||||||
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Title | Structure of a ssDNA bound to the outer DNA binding site of RAD52 | ||||||||||||||||||||||||
Components |
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Keywords | RECOMBINATION / Protein-DNA complex / ssDNA annealing protein / DNA repair protein | ||||||||||||||||||||||||
Function / homology | Function and homology information double-strand break repair via single-strand annealing / DNA double-strand break processing involved in repair via single-strand annealing / DNA recombinase assembly / mitotic recombination / regulation of nucleotide-excision repair / HDR through MMEJ (alt-NHEJ) / HDR through Single Strand Annealing (SSA) / SUMOylation of DNA damage response and repair proteins / protein-DNA complex / double-strand break repair via homologous recombination ...double-strand break repair via single-strand annealing / DNA double-strand break processing involved in repair via single-strand annealing / DNA recombinase assembly / mitotic recombination / regulation of nucleotide-excision repair / HDR through MMEJ (alt-NHEJ) / HDR through Single Strand Annealing (SSA) / SUMOylation of DNA damage response and repair proteins / protein-DNA complex / double-strand break repair via homologous recombination / double-strand break repair / single-stranded DNA binding / cellular response to oxidative stress / DNA recombination / protein-containing complex / DNA binding / nucleoplasm / identical protein binding / nucleus Similarity search - Function | ||||||||||||||||||||||||
Biological species | Homo sapiens (human) synthetic construct (others) | ||||||||||||||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3 Å | ||||||||||||||||||||||||
Authors | Saotome, M. / Saito, K. / Yasuda, T. / Sugiyama, S. / Kurumizaka, H. / Kagawa, W. | ||||||||||||||||||||||||
Funding support | Japan, 7items
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Citation | Journal: iScience / Year: 2018 Title: Structural Basis of Homology-Directed DNA Repair Mediated by RAD52 Authors: Saotome, M. / Saito, K. / Yasuda, T. / Ohtomo, H. / Sugiyama, S. / Nishimura, Y. / Kurumizaka, H. / Kagawa, W. | ||||||||||||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5xs0.cif.gz | 783.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5xs0.ent.gz | 651.3 KB | Display | PDB format |
PDBx/mmJSON format | 5xs0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xs/5xs0 ftp://data.pdbj.org/pub/pdb/validation_reports/xs/5xs0 | HTTPS FTP |
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-Related structure data
Related structure data | 5xrzC 1kn0S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 23713.785 Da / Num. of mol.: 22 / Fragment: UNP residues 1-212 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RAD52 / Plasmid: pET15b / Production host: Escherichia coli (E. coli) / Strain (production host): JM109(DE3) / References: UniProt: P43351 #2: DNA chain | | Mass: 1690.133 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #3: DNA chain | | Mass: 2268.497 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #4: DNA chain | | Mass: 2846.861 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #5: DNA chain | | Mass: 2557.679 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.59 Å3/Da / Density % sol: 52.55 % |
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Crystal grow | Temperature: 285 K / Method: vapor diffusion, hanging drop / Details: lithium citrate, PEG 3,350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: BL-17A / Wavelength: 0.98 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Mar 1, 2011 |
Radiation | Monochromator: NUMERICAL LINK TYPE SI(111) DOUBLE CRYSTAL MONOCHROMATOR Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 3→48.63 Å / Num. obs: 107806 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.8 % / Biso Wilson estimate: 91 Å2 / CC1/2: 0.997 / Rsym value: 0.083 / Net I/σ(I): 11.8 |
Reflection shell | Resolution: 3→3.05 Å / Redundancy: 3.8 % / Mean I/σ(I) obs: 1.8 / Num. unique obs: 5281 / CC1/2: 0.733 / Rsym value: 0.686 / % possible all: 98.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1KN0 Resolution: 3→48.529 Å / SU ML: 0.39 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 28.34 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→48.529 Å
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Refine LS restraints |
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LS refinement shell |
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