+Open data
-Basic information
Entry | Database: PDB / ID: 5x88 | ||||||
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Title | A crystal structure of cutinases from Malbranchea cinnamomea | ||||||
Components | cutinase | ||||||
Keywords | HYDROLASE / cutinase Malbranchea cinnamomea lipoidase monomer | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Malbranchea cinnamomea (fungus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.76 Å | ||||||
Authors | Jiang, Z. / Yang, S.Q. / You, X. / Huang, P. / Ma, J.W. | ||||||
Citation | Journal: Biotechnol Biofuels / Year: 2017 Title: High-level expression and characterization of a novel cutinase from Malbranchea cinnamomea suitable for butyl butyrate production. Authors: Duan, X. / Liu, Y. / You, X. / Jiang, Z. / Yang, S. / Yang, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5x88.cif.gz | 49.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5x88.ent.gz | 36.8 KB | Display | PDB format |
PDBx/mmJSON format | 5x88.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5x88_validation.pdf.gz | 416 KB | Display | wwPDB validaton report |
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Full document | 5x88_full_validation.pdf.gz | 416.7 KB | Display | |
Data in XML | 5x88_validation.xml.gz | 11 KB | Display | |
Data in CIF | 5x88_validation.cif.gz | 16.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/x8/5x88 ftp://data.pdbj.org/pub/pdb/validation_reports/x8/5x88 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 21201.758 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Malbranchea cinnamomea (fungus) / Production host: Komagataella pastoris GS115 (fungus) / References: UniProt: A0A1S6YJF3*PLUS, cutinase |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.96 Å3/Da / Density % sol: 37.35 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: PEG 3350,Potassium chloride |
-Data collection
Diffraction | Mean temperature: 80 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U / Wavelength: 1 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Oct 13, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.76→28.3 Å / Num. obs: 16910 / % possible obs: 98 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 6.8 % / Biso Wilson estimate: 15.65 Å2 / Rmerge(I) obs: 0.068 / Net I/σ(I): 266.8 |
Reflection shell | Resolution: 1.76→1.823 Å / Num. unique obs: 1626 |
-Processing
Software |
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Refinement | Resolution: 1.76→28.3 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 1.76→28.3 Å
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