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- PDB-5wu7: Crystal structure of GH57-type branching enzyme from hyperthermop... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5wu7 | ||||||
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Title | Crystal structure of GH57-type branching enzyme from hyperthermophilic archaeon Pyrococcus horikoshii | ||||||
![]() | Uncharacterized protein | ||||||
![]() | HYDROLASE / enzyme / amylase / glycogen branching enzyme | ||||||
Function / homology | ![]() alpha-glucan biosynthetic process / 1,4-alpha-glucan branching enzyme activity Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Na, S. / Jo, I. / Ha, N.-C. | ||||||
![]() | ![]() Title: Structural basis for the transglycosylase activity of a GH57-type glycogen branching enzyme from Pyrococcus horikoshii. Authors: Na, S. / Park, M. / Jo, I. / Cha, J. / Ha, N.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 228.7 KB | Display | ![]() |
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PDB format | ![]() | 183.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 450.5 KB | Display | ![]() |
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Full document | ![]() | 459.5 KB | Display | |
Data in XML | ![]() | 37.7 KB | Display | |
Data in CIF | ![]() | 51.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3n8tS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 66938.508 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: GOL Source: (gene. exp.) ![]() ![]() Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3 Gene: PH1386 / Production host: ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.33 Å3/Da / Density % sol: 47.32 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 9.3 Details: 20 mM citric acid, 10% (w/v) PEG 3350, 80 mM Bis-Tris propane pH 9.3, 2 mM tris (2-carboxyethyl) phosphine |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jun 2, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9796 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→33.521 Å / Num. obs: 44048 / % possible obs: 95 % / Redundancy: 5.5 % / Rmerge(I) obs: 0.13 / Net I/σ(I): 10.2 |
Reflection shell | Resolution: 2.3→2.34 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.383 / Mean I/σ(I) obs: 2.08 / % possible all: 72.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3N8T Resolution: 2.31→33.521 Å / SU ML: 0.3 / Cross valid method: FREE R-VALUE / σ(F): 1.45 / Phase error: 26.84
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.31→33.521 Å
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Refine LS restraints |
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LS refinement shell |
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