- PDB-5wq9: CryoEM structure of type II secretion system secretin GspD G453A ... -
+
データを開く
IDまたはキーワード:
読み込み中...
-
基本情報
登録情報
データベース: PDB / ID: 5wq9
タイトル
CryoEM structure of type II secretion system secretin GspD G453A mutant in Vibrio cholerae
要素
Type II secretion system protein D
キーワード
PROTEIN TRANSPORT / Secretin / G453A mutant / C15 symmetry / T2SS
機能・相同性
機能・相同性情報
protein secretion by the type II secretion system / type II protein secretion system complex / protein secretion / cell outer membrane / identical protein binding 類似検索 - 分子機能
Type II secretion system protein GspD / GspD/PilQ family / Bacterial type II secretion system protein D signature. / Type II secretion system protein GspD, conserved site / NolW-like / Bacterial type II/III secretion system short domain / NolW-like superfamily / Type II/III secretion system / Bacterial type II and III secretion system protein 類似検索 - ドメイン・相同性
ジャーナル: Nat Struct Mol Biol / 年: 2017 タイトル: Structural insights into the secretin translocation channel in the type II secretion system. 著者: Zhaofeng Yan / Meng Yin / Dandan Xu / Yongqun Zhu / Xueming Li / 要旨: The secretin GspD of the type II secretion system (T2SS) forms a channel across the outer membrane in Gram-negative bacteria to transport substrates from the periplasm to the extracellular milieu. ...The secretin GspD of the type II secretion system (T2SS) forms a channel across the outer membrane in Gram-negative bacteria to transport substrates from the periplasm to the extracellular milieu. The lack of an atomic-resolution structure of the GspD channel hinders the investigation of substrate translocation mechanism of T2SS. Here we report cryo-EM structures of two GspD channels (∼1 MDa), from Escherichia coli K12 and Vibrio cholerae, at ∼3 Å resolution. The structures reveal a pentadecameric channel architecture, wherein three rings of GspD N domains form the periplasmic channel. The secretin domain constitutes a novel double β-barrel channel, with at least 60 β-strands in each barrel, and is stabilized by S domains. The outer membrane channel is sealed by β-strand-enriched gates. On the basis of the partially open state captured, we proposed a detailed gate-opening mechanism. Our structures provide a structural basis for understanding the secretin superfamily and the mechanism of substrate translocation in T2SS.
A: Type II secretion system protein D B: Type II secretion system protein D C: Type II secretion system protein D D: Type II secretion system protein D E: Type II secretion system protein D F: Type II secretion system protein D G: Type II secretion system protein D H: Type II secretion system protein D I: Type II secretion system protein D J: Type II secretion system protein D K: Type II secretion system protein D L: Type II secretion system protein D M: Type II secretion system protein D N: Type II secretion system protein D O: Type II secretion system protein D