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Yorodumi- PDB-5woz: NMR solution structure of Rtt103 (RTT) protein using two 4D-spectra -
+Open data
-Basic information
Entry | Database: PDB / ID: 5woz | ||||||
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Title | NMR solution structure of Rtt103 (RTT) protein using two 4D-spectra | ||||||
Components | Regulator of Ty1 transposition protein 103 | ||||||
Keywords | TRANSCRIPTION REGULATOR / RNA polymerase II core binder | ||||||
Function / homology | Function and homology information RNA polymerase II C-terminal domain phosphoserine binding / retrotransposon silencing / mRNA 3'-end processing / RNA polymerase II transcribes snRNA genes / RNA polymerase II complex binding / site of double-strand break / chromatin / DNA binding / nucleus Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | SOLUTION NMR / na | ||||||
Authors | Evangelidis, T. / Nerli, S. / Sgourakis, N.G. / Tripsianes, K. | ||||||
Citation | Journal: Nat Commun / Year: 2018 Title: Automated NMR resonance assignments and structure determination using a minimal set of 4D spectra. Authors: Evangelidis, T. / Nerli, S. / Novacek, J. / Brereton, A.E. / Karplus, P.A. / Dotas, R.R. / Venditti, V. / Sgourakis, N.G. / Tripsianes, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5woz.cif.gz | 434.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5woz.ent.gz | 361.7 KB | Display | PDB format |
PDBx/mmJSON format | 5woz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5woz_validation.pdf.gz | 322.9 KB | Display | wwPDB validaton report |
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Full document | 5woz_full_validation.pdf.gz | 356.1 KB | Display | |
Data in XML | 5woz_validation.xml.gz | 17.8 KB | Display | |
Data in CIF | 5woz_validation.cif.gz | 30.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wo/5woz ftp://data.pdbj.org/pub/pdb/validation_reports/wo/5woz | HTTPS FTP |
-Related structure data
Related structure data | 5wotC 5woxC 5woyC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 16132.574 Da / Num. of mol.: 1 / Fragment: residues 5-131 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / Gene: RTT103, YDR289C / Production host: Escherichia coli (E. coli) / References: UniProt: Q05543 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution Contents: 0.8 mM [U-13C; U-15N] Rtt103 (RTT) protein, 95% H2O/5% D2O Label: 13C_15N_sample / Solvent system: 95% H2O/5% D2O |
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Sample | Conc.: 0.8 mM / Component: Rtt103 (RTT) protein / Isotopic labeling: [U-13C; U-15N] |
Sample conditions | Ionic strength: 35 mM / Label: conditions_1 / pH: 6.8 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 850 MHz |
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-Processing
NMR software |
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Refinement | Method: na / Software ordinal: 2 | ||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 |