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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 5w3g | ||||||
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タイトル | Solution Structure of ETS Transcription Factor PU.1 | ||||||
![]() | Transcription factor PU.1 | ||||||
![]() | DNA BINDING PROTEIN / winged helix-turn-helix | ||||||
機能・相同性 | ![]() positive regulation of antifungal innate immune response / regulation of myeloid progenitor cell differentiation / positive regulation of myeloid dendritic cell chemotaxis / anatomical structure regression / pro-T cell differentiation / negative regulation of neutrophil degranulation / follicular B cell differentiation / myeloid leukocyte differentiation / positive regulation of microglial cell mediated cytotoxicity / germinal center B cell differentiation ...positive regulation of antifungal innate immune response / regulation of myeloid progenitor cell differentiation / positive regulation of myeloid dendritic cell chemotaxis / anatomical structure regression / pro-T cell differentiation / negative regulation of neutrophil degranulation / follicular B cell differentiation / myeloid leukocyte differentiation / positive regulation of microglial cell mediated cytotoxicity / germinal center B cell differentiation / lymphocyte differentiation / TRAIL-activated apoptotic signaling pathway / granulocyte differentiation / endothelial to hematopoietic transition / negative regulation of adipose tissue development / apoptotic process involved in blood vessel morphogenesis / pericyte cell differentiation / lymphoid progenitor cell differentiation / negative regulation of MHC class II biosynthetic process / immature B cell differentiation / myeloid dendritic cell differentiation / defense response to tumor cell / vasculature development / positive regulation of p38MAPK cascade / oncogene-induced cell senescence / negative regulation of non-canonical NF-kappaB signal transduction / negative regulation of protein localization to chromatin / positive regulation of B cell differentiation / STAT family protein binding / interleukin-6-mediated signaling pathway / NFAT protein binding / cellular response to ethanol / macrophage differentiation / somatic stem cell population maintenance / cis-regulatory region sequence-specific DNA binding / negative regulation of canonical NF-kappaB signal transduction / transcription initiation-coupled chromatin remodeling / transforming growth factor beta receptor signaling pathway / protein sequestering activity / osteoclast differentiation / lipopolysaccharide-mediated signaling pathway / erythrocyte differentiation / regulation of erythrocyte differentiation / positive regulation of miRNA transcription / DNA-binding transcription repressor activity, RNA polymerase II-specific / histone deacetylase binding / DNA-binding transcription activator activity, RNA polymerase II-specific / transcription regulator complex / DNA-binding transcription factor binding / sequence-specific DNA binding / RNA polymerase II-specific DNA-binding transcription factor binding / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of gene expression / negative regulation of DNA-templated transcription / chromatin binding / positive regulation of gene expression / regulation of transcription by RNA polymerase II / regulation of DNA-templated transcription / chromatin / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / RNA binding / nucleoplasm / nucleus 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
![]() | Lau, D.K.W. / Okon, M. / McIntosh, L.P. | ||||||
![]() | ![]() タイトル: Conserved protein dynamics within the ETS transcription factor family 著者: Lau, D.K.W. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 423.2 KB | 表示 | ![]() |
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-検証レポート
文書・要旨 | ![]() | 432.3 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 484.9 KB | 表示 | |
XML形式データ | ![]() | 19.6 KB | 表示 | |
CIF形式データ | ![]() | 32.1 KB | 表示 | |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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NMR アンサンブル |
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要素
#1: タンパク質 | 分子量: 12932.241 Da / 分子数: 1 / 断片: residues 167-272 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR実験 |
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試料調製
詳細 | タイプ: solution 内容: 0.3 mM [U-99% 13C; U-99% 15N] DNA binding protein, 95% H2O/5% D2O Label: 15N 13C / 溶媒系: 95% H2O/5% D2O |
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試料 | 濃度: 0.3 mM / 構成要素: DNA binding protein / Isotopic labeling: [U-99% 13C; U-99% 15N] |
試料状態 | イオン強度: 150mM KCL mM / Label: conditions_1 / pH: 5.5 / 圧: 1 atm / 温度: 298 K |
-NMR測定
NMRスペクトロメーター | タイプ: Bruker AVANCE III / 製造業者: Bruker / モデル: AVANCE III / 磁場強度: 850 MHz |
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解析
NMR software |
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精密化 | 手法: simulated annealing / ソフトェア番号: 1 / 詳細: NMRe | ||||||||||||||||||
代表構造 | 選択基準: lowest energy | ||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 10 |