- PDB-5w2c: Structure of human DNA polymerase kappa in complex with Lucidin-d... -
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基本情報
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データベース: PDB / ID: 5w2c
タイトル
Structure of human DNA polymerase kappa in complex with Lucidin-derived DNA adduct and incoming dAMPNPP
要素
DNA (5'-D(*CP*TP*AP*TP*(LDG)P*TP*CP*GP*AP*TP*CP*CP*G)-3')
DNA (5'-D(P*CP*GP*GP*AP*TP*CP*GP*AP*C)-3')
DNA polymerase kappa
キーワード
REPLICATION / Translesion synthesis / Lucidin-derived DNA adduct / DNA polymerase kappa / mutagenesis
機能・相同性
機能・相同性情報
nucleotide-excision repair, DNA gap filling / error-prone translesion synthesis / Translesion synthesis by POLK / Gap-filling DNA repair synthesis and ligation in GG-NER / Termination of translesion DNA synthesis / HDR through Homologous Recombination (HRR) / Dual Incision in GG-NER / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / cellular response to UV ...nucleotide-excision repair, DNA gap filling / error-prone translesion synthesis / Translesion synthesis by POLK / Gap-filling DNA repair synthesis and ligation in GG-NER / Termination of translesion DNA synthesis / HDR through Homologous Recombination (HRR) / Dual Incision in GG-NER / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / cellular response to UV / DNA replication / damaged DNA binding / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / nuclear body / DNA repair / DNA damage response / nucleoplasm / nucleus / metal ion binding / cytosol 類似検索 - 分子機能
DNA polymerase; domain 1 - #810 / Rad18-like CCHC zinc finger / DNA polymerase type-Y, HhH motif / IMS family HHH motif / DNA polymerase IV / DNA polymerase, Y-family, little finger domain / MutS, DNA mismatch repair protein, domain I - #60 / Rad18, zinc finger UBZ4-type / Zinc finger UBZ4-type profile. / MutS, DNA mismatch repair protein, domain I ...DNA polymerase; domain 1 - #810 / Rad18-like CCHC zinc finger / DNA polymerase type-Y, HhH motif / IMS family HHH motif / DNA polymerase IV / DNA polymerase, Y-family, little finger domain / MutS, DNA mismatch repair protein, domain I - #60 / Rad18, zinc finger UBZ4-type / Zinc finger UBZ4-type profile. / MutS, DNA mismatch repair protein, domain I / DNA polymerase, Y-family, little finger domain / impB/mucB/samB family C-terminal domain / UmuC domain / DNA polymerase, Y-family, little finger domain superfamily / impB/mucB/samB family / UmuC domain profile. / Reverse transcriptase/Diguanylate cyclase domain / Dna Ligase; domain 1 / DNA polymerase; domain 1 / Reverse transcriptase/Diguanylate cyclase domain / Alpha-Beta Plaits / DNA/RNA polymerase superfamily / 2-Layer Sandwich / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Alpha / Alpha Beta 類似検索 - ドメイン・相同性
Chem-DZ4 / DI(HYDROXYETHYL)ETHER / DNA / DNA (> 10) / DNA polymerase kappa 類似検索 - 構成要素
A: DNA polymerase kappa B: DNA polymerase kappa C: DNA (5'-D(P*CP*GP*GP*AP*TP*CP*GP*AP*C)-3') D: DNA (5'-D(*CP*TP*AP*TP*(LDG)P*TP*CP*GP*AP*TP*CP*CP*G)-3') P: DNA (5'-D(P*CP*GP*GP*AP*TP*CP*GP*AP*C)-3') T: DNA (5'-D(*CP*TP*AP*TP*(LDG)P*TP*CP*GP*AP*TP*CP*CP*G)-3') ヘテロ分子
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.9792 Å / 相対比: 1
反射
解像度: 2.5→50.01 Å / Num. obs: 48405 / % possible obs: 99.5 % / 冗長度: 4.4 % / CC1/2: 0.999 / Rmerge(I) obs: 0.044 / Net I/σ(I): 16.3
反射 シェル
解像度: 2.5→2.64 Å / 冗長度: 4.5 % / Rmerge(I) obs: 0.477 / Mean I/σ(I) obs: 2.7 / Num. unique obs: 7002 / CC1/2: 0.937 / % possible all: 99.7
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解析
ソフトウェア
名称
バージョン
分類
REFMAC
5.8.0135
精密化
XDS
データ削減
SCALA
データスケーリング
REFMAC
位相決定
精密化
解像度: 2.5→50.01 Å / Cor.coef. Fo:Fc: 0.959 / Cor.coef. Fo:Fc free: 0.948 / SU B: 15.336 / SU ML: 0.298 / 交差検証法: THROUGHOUT / ESU R: 0.385 / ESU R Free: 0.263 / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS