+Open data
-Basic information
Entry | Database: PDB / ID: 5vr4 | ||||||||||||||||||||
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Title | RNA octamer containing 2'-F-4'-OMe U. | ||||||||||||||||||||
Components | RNA (5'-R(*Keywords | RNA | Function / homology | COBALT TETRAAMMINE ION / RNA | Function and homology information Biological species | synthetic construct (others) | Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.5 Å | Authors | Harp, J.M. / Egli, M. | Citation | Journal: J. Am. Chem. Soc. / Year: 2017 | Title: 4'-C-Methoxy-2'-deoxy-2'-fluoro Modified Ribonucleotides Improve Metabolic Stability and Elicit Efficient RNAi-Mediated Gene Silencing. Authors: Malek-Adamian, E. / Guenther, D.C. / Matsuda, S. / Martinez-Montero, S. / Zlatev, I. / Harp, J. / Burai Patrascu, M. / Foster, D.J. / Fakhoury, J. / Perkins, L. / Moitessier, N. / Manoharan, ...Authors: Malek-Adamian, E. / Guenther, D.C. / Matsuda, S. / Martinez-Montero, S. / Zlatev, I. / Harp, J. / Burai Patrascu, M. / Foster, D.J. / Fakhoury, J. / Perkins, L. / Moitessier, N. / Manoharan, R.M. / Taneja, N. / Bisbe, A. / Charisse, K. / Maier, M. / Rajeev, K.G. / Egli, M. / Manoharan, M. / Damha, M.J. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5vr4.cif.gz | 58.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5vr4.ent.gz | 43.2 KB | Display | PDB format |
PDBx/mmJSON format | 5vr4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vr/5vr4 ftp://data.pdbj.org/pub/pdb/validation_reports/vr/5vr4 | HTTPS FTP |
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-Related structure data
Related structure data | 3p4aS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: RNA chain | Mass: 2558.578 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #2: Chemical | ChemComp-CON / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.08 Å3/Da / Density % sol: 40.51 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.6 Details: 0.5 mM RNA, 10 mM Cobalt(III) hexamine, 6 mM sodium chloride, 40 mM potassium chloride, 5% MPD, 20 mM sodium cacodylate equilibrated against reservoir containing 40% MPD Temp details: Room temperature |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 0.97856 Å |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Jul 1, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97856 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→30 Å / Num. obs: 26152 / % possible obs: 99.6 % / Redundancy: 7.3 % / Biso Wilson estimate: 25.4 Å2 / Rmerge(I) obs: 0.067 / Rpim(I) all: 0.027 / Net I/σ(I): 21.05 |
Reflection shell | Resolution: 1.5→1.53 Å / Redundancy: 7.3 % / Rmerge(I) obs: 0.795 / Mean I/σ(I) obs: 2.73 / CC1/2: 0.943 / Rpim(I) all: 0.317 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3P4A Resolution: 1.5→29.481 Å / SU ML: 0.19 / Cross valid method: THROUGHOUT / σ(F): 0.17 / Phase error: 24.16
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.5→29.481 Å
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Refine LS restraints |
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LS refinement shell |
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