+Open data
-Basic information
Entry | Database: PDB / ID: 5vge | ||||||
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Title | Crystal structure of HLA-C*07:02 in complex with RYR peptide | ||||||
Components |
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Keywords | IMMUNE SYSTEM / Human leukocyte antigen Major histocompatibility complex Antigen presentation | ||||||
Function / homology | Function and homology information antigen processing and presentation / Chromatin modifying enzymes / epigenetic regulation of gene expression / TAP binding / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent / antigen processing and presentation of endogenous peptide antigen via MHC class Ib / telomere organization / RNA Polymerase I Promoter Opening / Interleukin-7 signaling / Assembly of the ORC complex at the origin of replication ...antigen processing and presentation / Chromatin modifying enzymes / epigenetic regulation of gene expression / TAP binding / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent / antigen processing and presentation of endogenous peptide antigen via MHC class Ib / telomere organization / RNA Polymerase I Promoter Opening / Interleukin-7 signaling / Assembly of the ORC complex at the origin of replication / DNA methylation / Condensation of Prophase Chromosomes / secretory granule membrane / HCMV Late Events / Chromatin modifications during the maternal to zygotic transition (MZT) / ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression / SIRT1 negatively regulates rRNA expression / positive regulation of ferrous iron binding / positive regulation of transferrin receptor binding / PRC2 methylates histones and DNA / early endosome lumen / positive regulation of receptor binding / Nef mediated downregulation of MHC class I complex cell surface expression / DAP12 interactions / negative regulation of receptor binding / Defective pyroptosis / HDACs deacetylate histones / lumenal side of endoplasmic reticulum membrane / RNA Polymerase I Promoter Escape / Endosomal/Vacuolar pathway / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / Transcriptional regulation by small RNAs / Formation of the beta-catenin:TCF transactivating complex / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / cellular response to iron(III) ion / Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 / negative regulation of forebrain neuron differentiation / NoRC negatively regulates rRNA expression / ER to Golgi transport vesicle membrane / B-WICH complex positively regulates rRNA expression / HDMs demethylate histones / peptide antigen assembly with MHC class I protein complex / response to molecule of bacterial origin / regulation of erythrocyte differentiation / regulation of iron ion transport / MHC class I peptide loading complex / HFE-transferrin receptor complex / T cell mediated cytotoxicity / cellular response to iron ion / PKMTs methylate histone lysines / antigen processing and presentation of endogenous peptide antigen via MHC class I / RMTs methylate histone arginines / positive regulation of T cell cytokine production / Meiotic recombination / Pre-NOTCH Transcription and Translation / MHC class I protein complex / nucleosome assembly / multicellular organismal-level iron ion homeostasis / positive regulation of T cell mediated cytotoxicity / peptide antigen assembly with MHC class II protein complex / Activation of anterior HOX genes in hindbrain development during early embryogenesis / HCMV Early Events / negative regulation of neurogenesis / MHC class II protein complex / positive regulation of receptor-mediated endocytosis / cellular response to nicotine / Transcriptional regulation of granulopoiesis / structural constituent of chromatin / recycling endosome membrane / phagocytic vesicle membrane / specific granule lumen / peptide antigen binding / positive regulation of cellular senescence / antigen processing and presentation of exogenous peptide antigen via MHC class II / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / Interferon gamma signaling / positive regulation of immune response / negative regulation of epithelial cell proliferation / Modulation by Mtb of host immune system / positive regulation of T cell activation / nucleosome / Interferon alpha/beta signaling / sensory perception of smell / negative regulation of neuron projection development / tertiary granule lumen / DAP12 signaling / MHC class II protein complex binding / late endosome membrane / T cell differentiation in thymus / gene expression / RUNX1 regulates transcription of genes involved in differentiation of HSCs / chromatin organization / positive regulation of protein binding / Factors involved in megakaryocyte development and platelet production / HATs acetylate histones / ER-Phagosome pathway / iron ion transport / Senescence-Associated Secretory Phenotype (SASP) / protein refolding Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å | ||||||
Authors | Mobbs, J.I. / Vivian, J.P. / Gras, S. / Rossjohn, J. | ||||||
Citation | Journal: Nat Commun / Year: 2017 Title: Structural and regulatory diversity shape HLA-C protein expression levels. Authors: Kaur, G. / Gras, S. / Mobbs, J.I. / Vivian, J.P. / Cortes, A. / Barber, T. / Kuttikkatte, S.B. / Jensen, L.T. / Attfield, K.E. / Dendrou, C.A. / Carrington, M. / McVean, G. / Purcell, A.W. / ...Authors: Kaur, G. / Gras, S. / Mobbs, J.I. / Vivian, J.P. / Cortes, A. / Barber, T. / Kuttikkatte, S.B. / Jensen, L.T. / Attfield, K.E. / Dendrou, C.A. / Carrington, M. / McVean, G. / Purcell, A.W. / Rossjohn, J. / Fugger, L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5vge.cif.gz | 94.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5vge.ent.gz | 70.1 KB | Display | PDB format |
PDBx/mmJSON format | 5vge.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vg/5vge ftp://data.pdbj.org/pub/pdb/validation_reports/vg/5vge | HTTPS FTP |
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-Related structure data
Related structure data | 5vgdC 4nt6S C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 31654.715 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) / References: UniProt: B4DVY0, UniProt: P10321*PLUS | ||
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#2: Protein | Mass: 11879.356 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: B2M, CDABP0092, HDCMA22P / Production host: Escherichia coli (E. coli) / References: UniProt: P61769 | ||
#3: Protein/peptide | Mass: 1034.213 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: P68431*PLUS | ||
#4: Chemical | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50.88 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 0.1 M HEPES pH 8.5 2 mM ZnSO4 28 % jeffamine ED-2001 (Hampton) |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX1 / Wavelength: 0.954 Å | |||||||||||||||
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Dec 7, 2016 | |||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength | Wavelength: 0.954 Å / Relative weight: 1 | |||||||||||||||
Reflection twin |
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Reflection | Resolution: 2.6→43.16 Å / Num. obs: 13655 / % possible obs: 100 % / Redundancy: 11.3 % / Rpim(I) all: 0.05 / Net I/σ(I): 14.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4NT6 Resolution: 2.6→43.16 Å / Cor.coef. Fo:Fc: 0.954 / Cor.coef. Fo:Fc free: 0.934 / SU B: 8.613 / SU ML: 0.196 / Cross valid method: THROUGHOUT / ESU R: 0.288 / ESU R Free: 0.061 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 55.984 Å2
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Refinement step | Cycle: 1 / Resolution: 2.6→43.16 Å
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