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- PDB-5va1: Cryo-EM structure of the human ether-a-go-go related K+ channel -

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Basic information

Entry
Database: PDB / ID: 5va1
TitleCryo-EM structure of the human ether-a-go-go related K+ channel
DescriptorPotassium voltage-gated channel
subfamily H (Eag-related)
member 2
KeywordsTRANSPORT PROTEIN / K+ channel / PAS / CNBHD / voltage sensor / selectivity filter
Specimen sourceHomo sapiens / human
MethodElectron microscopy (3.7 A resolution / Single particle)
AuthorsWang, W.W. / MacKinnon, R.
CitationCell, 2017, 169, 422-430.e10

Cell, 2017, 169, 422-430.e10 StrPapers
Cryo-EM Structure of the Open Human Ether-à-go-go-Related K(+) Channel hERG.
Weiwei Wang / Roderick MacKinnon

DateDeposition: Mar 24, 2017 / Release: May 3, 2017

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Assembly

Deposited unit
A: Potassium voltage-gated channel subfamily H member 2


Theoretical massNumber of molelcules
Total (without water)88,9021
Polyers88,9021
Non-polymers00
Water0
#1
A: Potassium voltage-gated channel subfamily H member 2

A: Potassium voltage-gated channel subfamily H member 2

A: Potassium voltage-gated channel subfamily H member 2

A: Potassium voltage-gated channel subfamily H member 2


Theoretical massNumber of molelcules
Total (without water)355,6074
Polyers355,6074
Non-polymers00
Water0
TypeNameSymmetry operationNumber
point symmetry operation4
#2


  • idetical with deposited unit in distinct coordinate
  • point asymmetric unit
TypeNameSymmetry operationNumber
point symmetry operation1
#3


  • idetical with deposited unit in distinct coordinate
  • point asymmetric unit, std point frame
TypeNameSymmetry operationNumber
transform to point frame1

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Components

#1: Polypeptide(L)Potassium voltage-gated channel subfamily H member 2 / Eag homolog / Ether-a-go-go-related gene potassium channel 1 / hERG1 / Voltage-gated potassium channel subunit Kv11.1


Mass: 88901.664 Da / Num. of mol.: 1 / Fragment: UNP residues 1-140,381-870,1006-1159 / Source: (gene. exp.) Homo sapiens / human / References: UniProt: Q12809

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentReconstruction method: SINGLE PARTICLE

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Sample preparation

ComponentName: human ether-a-go-go related K+ channel hERG
Details: Truncated hERG construct hERGTs (amino acid residues 141-380 and 871-1005 deleted)
Type: ORGANELLE OR CELLULAR COMPONENT
Buffer solutionDetails: pH 7.4, adjusted with NaOH
Specimen supportGrid material: GOLD / Grid mesh size: 400 /inch. / Grid type: Quantifoil R1.2/1.3
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 98 / Details: one blot: 3 second blot time, 0 blot force

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Electron microscopy imaging

MicroscopyMicroscope model: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Calibrated magnification: 38461 X / Nominal defocus max: 3500 nm / Nominal defocus min: 800 nm / Cs: 2.7 mm
Specimen holderSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER

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Processing

3D reconstructionResolution: 3.7 A / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 213255 / Number of class averages: 3
Atomic model buildingOverall b value: 140 / Ref protocol: AB INITIO MODEL / Ref space: RECIPROCAL / Target criteria: Fourier Shell Correlation

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