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- PDB-5v8a: Crystal structure of Influenza A virus matrix protein M1 (NLS-88R... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5v8a | |||||||||
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Title | Crystal structure of Influenza A virus matrix protein M1 (NLS-88R, pH 7.3) | |||||||||
![]() | Matrix protein 1 | |||||||||
![]() | VIRAL PROTEIN / Influenza A / Matrix protein / NLS-88R mutant / pH 7.3 | |||||||||
Function / homology | ![]() viral budding from plasma membrane / structural constituent of virion / host cell nucleus / virion membrane / RNA binding / membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Musayev, F.N. / Safo, M.K. / Desai, U.R. / Xie, H. / Mosier, P.D. / Zhou, Q. / Chiang, M.-J. / Kosikova, M. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Maintaining pH-dependent conformational flexibility of M1 is critical for efficient influenza A virus replication. Authors: Chiang, M.J. / Musayev, F.N. / Kosikova, M. / Lin, Z. / Gao, Y. / Mosier, P.D. / Althufairi, B. / Ye, Z. / Zhou, Q. / Desai, U.R. / Xie, H. / Safo, M.K. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 42.1 KB | Display | ![]() |
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PDB format | ![]() | 27.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 425.5 KB | Display | ![]() |
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Full document | ![]() | 426.8 KB | Display | |
Data in XML | ![]() | 7.4 KB | Display | |
Data in CIF | ![]() | 8.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5v6gC ![]() 5v7bC ![]() 5v7sC ![]() 1ea3S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 19098.055 Da / Num. of mol.: 1 / Mutation: G88R, R101S, R105S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: A/Wilson-Smith/1933 H1N1 / Plasmid: pET30a / Production host: ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.57 Å3/Da / Density % sol: 21.75 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.3 Details: Protein solution: 10 mg/ml in K2HPO4/KH2PO4, 0.2M NaCl, 10bME, pH 7.2. Reservoir solution: 0.2M NaF, 20% PEG-3350, pH 7.3 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Apr 26, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5419 Å / Relative weight: 1 |
Reflection | Resolution: 3→28.7 Å / Num. obs: 2187 / % possible obs: 94.5 % / Redundancy: 2.83 % / Biso Wilson estimate: 73.06 Å2 / Rmerge(I) obs: 0.081 / Net I/σ(I): 10.9 |
Reflection shell | Resolution: 3→3.11 Å / Redundancy: 2.97 % / Rmerge(I) obs: 0.512 / Num. unique all: 204 / Num. unique obs: 269 / % possible all: 93.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1EA3 Resolution: 3→21.926 Å / SU ML: 0.52 / Cross valid method: THROUGHOUT / σ(F): 1.99 / Phase error: 42.26 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→21.926 Å
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Refine LS restraints |
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LS refinement shell |
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